AIM: To investigate the role of glutathione S-transferase (GST) and matrix metalloproteinase-9 (MMP-9) expres-sions in the development and progression of reflux es-ophagitis-Barrett’s metaplasia-dysplasia-adenocarcin...AIM: To investigate the role of glutathione S-transferase (GST) and matrix metalloproteinase-9 (MMP-9) expres-sions in the development and progression of reflux es-ophagitis-Barrett’s metaplasia-dysplasia-adenocarcinoma sequence in the esophagus.METHODS: GST and MMP-9 expressions were analyzed in 51 paraffin-embedded tissue samples by immunohisto-chemistry including patients with reflux esophagitis (n = 7), Barrett’s metaplasia (n = 14), Barrett and esophagi-tis (n = 8), Barrett and dysplasia (n = 7), esophageal adenocarcinoma (n = 8) and a control group without any histological changes (n = 7). Immunostaining was determined semiquantitatively. Statistical analysis with one-way ANOVA, LSD test and correlation analysis were performed. P value of < 0.05 was considered significant.RESULTS: GST expression was significantly higher while MMP-9 expression was significantly lower in control group compared to Barrett’s metaplasia and the other groups. No major changes were observed between Bar-rett, esophagitis, and Barrett and concomitant esophagi-tis. Barrett and concomitant dysplasia, and adenocarci-noma revealed a significant lower expression of GST and higher levels of MMP-9 compared to all other groups. Adenocarcinoma showed almost no expression of GST and significantly higher levels of MMP-9 than Barrett and concomitant dysplasia. Alterations of GST and MMP-9 were inversely correlated (r = - 0.82).CONCLUSION: Decreased GST and increased ex-pression of MMP-9 in Barrett’s metaplasia-dysplasia-adenocarcinoma sequence as compared to normal tissue suggest their association with esophageal tumorigenesis. Loss of GST and gain of MMP-9 in Barrett with dyspla-sia compared to non-dysplastic metaplasia indicate that these alterations may be early events in carcinogenesis. Quantification of these parameters in Barrett’s esopha-gus might be useful to identify patients at higher risk for progression to cancer.展开更多
FABP4(Fatty acid binding protein 4)参与细胞内脂肪酸的转运和脂肪酸代谢,是当前研究动物脂肪沉积与代谢的热门候选基因。为了研究FABP4基因在绵羊尾脂沉积与代谢中的作用,文章采用生物信息学方法分析了FABP4氨基酸序列在各物种中的...FABP4(Fatty acid binding protein 4)参与细胞内脂肪酸的转运和脂肪酸代谢,是当前研究动物脂肪沉积与代谢的热门候选基因。为了研究FABP4基因在绵羊尾脂沉积与代谢中的作用,文章采用生物信息学方法分析了FABP4氨基酸序列在各物种中的保守性;利用半定量RT-PCR方法检测了该基因在阿勒泰羊主要组织中的表达;采用饥饿法成功建立了模拟阿勒泰羊尾脂沉积与代谢的动物模型,利用q PCR和i TRAQ(isobaric tags for relative and absolute quantitation)技术同时验证FABP4基因m RNA和蛋白在阿勒泰羊非饥饿组与饥饿组尾脂中的表达变化。序列分析结果表明,绵羊FABP4氨基酸序列在物种间高度保守,提示FABP4基因可能由于其重要的生物功能而在进化中表现出其物种的保守性。组织表达谱结果显示,FABP4 m RNA在阿勒泰羊肠脂与尾脂中均高丰度表达,暗示FABP4基因可能在脂肪中行驶着重要的生理生化功能。q PCR与i TRAQ结果显示,FABP4 m RNA与蛋白在两种极端条件下尾脂中的表达差异均不显著(P>0.05),表明FABP4基因可能不是绵羊尾脂沉积与代谢两种极端差异表型的决定基因。以上研究结果为进一步研究FABP4基因在绵羊尾脂中的生物功能奠定了基础。展开更多
文摘AIM: To investigate the role of glutathione S-transferase (GST) and matrix metalloproteinase-9 (MMP-9) expres-sions in the development and progression of reflux es-ophagitis-Barrett’s metaplasia-dysplasia-adenocarcinoma sequence in the esophagus.METHODS: GST and MMP-9 expressions were analyzed in 51 paraffin-embedded tissue samples by immunohisto-chemistry including patients with reflux esophagitis (n = 7), Barrett’s metaplasia (n = 14), Barrett and esophagi-tis (n = 8), Barrett and dysplasia (n = 7), esophageal adenocarcinoma (n = 8) and a control group without any histological changes (n = 7). Immunostaining was determined semiquantitatively. Statistical analysis with one-way ANOVA, LSD test and correlation analysis were performed. P value of < 0.05 was considered significant.RESULTS: GST expression was significantly higher while MMP-9 expression was significantly lower in control group compared to Barrett’s metaplasia and the other groups. No major changes were observed between Bar-rett, esophagitis, and Barrett and concomitant esophagi-tis. Barrett and concomitant dysplasia, and adenocarci-noma revealed a significant lower expression of GST and higher levels of MMP-9 compared to all other groups. Adenocarcinoma showed almost no expression of GST and significantly higher levels of MMP-9 than Barrett and concomitant dysplasia. Alterations of GST and MMP-9 were inversely correlated (r = - 0.82).CONCLUSION: Decreased GST and increased ex-pression of MMP-9 in Barrett’s metaplasia-dysplasia-adenocarcinoma sequence as compared to normal tissue suggest their association with esophageal tumorigenesis. Loss of GST and gain of MMP-9 in Barrett with dyspla-sia compared to non-dysplastic metaplasia indicate that these alterations may be early events in carcinogenesis. Quantification of these parameters in Barrett’s esopha-gus might be useful to identify patients at higher risk for progression to cancer.
文摘FABP4(Fatty acid binding protein 4)参与细胞内脂肪酸的转运和脂肪酸代谢,是当前研究动物脂肪沉积与代谢的热门候选基因。为了研究FABP4基因在绵羊尾脂沉积与代谢中的作用,文章采用生物信息学方法分析了FABP4氨基酸序列在各物种中的保守性;利用半定量RT-PCR方法检测了该基因在阿勒泰羊主要组织中的表达;采用饥饿法成功建立了模拟阿勒泰羊尾脂沉积与代谢的动物模型,利用q PCR和i TRAQ(isobaric tags for relative and absolute quantitation)技术同时验证FABP4基因m RNA和蛋白在阿勒泰羊非饥饿组与饥饿组尾脂中的表达变化。序列分析结果表明,绵羊FABP4氨基酸序列在物种间高度保守,提示FABP4基因可能由于其重要的生物功能而在进化中表现出其物种的保守性。组织表达谱结果显示,FABP4 m RNA在阿勒泰羊肠脂与尾脂中均高丰度表达,暗示FABP4基因可能在脂肪中行驶着重要的生理生化功能。q PCR与i TRAQ结果显示,FABP4 m RNA与蛋白在两种极端条件下尾脂中的表达差异均不显著(P>0.05),表明FABP4基因可能不是绵羊尾脂沉积与代谢两种极端差异表型的决定基因。以上研究结果为进一步研究FABP4基因在绵羊尾脂中的生物功能奠定了基础。
