目的探讨表儿茶酸[(-)-epicatechin,EC]对脑损伤(traumatic brain injury,TBI)小鼠的神经修复作用及机制。方法建立小鼠自由落体脑外伤动物模型,将50只C57BL/6小鼠随机(随机数字法)分为脑损伤+生理盐水(Veh)组,脑损伤+表儿茶酸(EC)组(E...目的探讨表儿茶酸[(-)-epicatechin,EC]对脑损伤(traumatic brain injury,TBI)小鼠的神经修复作用及机制。方法建立小鼠自由落体脑外伤动物模型,将50只C57BL/6小鼠随机(随机数字法)分为脑损伤+生理盐水(Veh)组,脑损伤+表儿茶酸(EC)组(EC组和Veh组各死亡1只,每组24只)。术后3 d,采用ELISA方法检测炎症因子IL-1β和TNF-ɑ的表达,PI染色检测分析损伤区坏死细胞数;水迷宫实验观察术后28 d小鼠空间学习记忆能力,qRT-PCR检测神经营养因子BDNF和NGF的表达,免疫荧光染色检测NeuN的表达,EdU染色观察SGZ区神经再生。结果与Veh组相比较,EC治疗组明显改善小鼠的空间学习记忆能力,平台象限停留时间增加[24 d:(26.333±5.037)% vs (26.583±5.802)%,P=0.938;25 d:(33.300±4.724)% vs (29.767±3.347)%,P=0.166;26 d:(41.017±7.246)% vs (32.800±8.145)%,P=0.095l;27 d:(48.017±7.424)% vs (35.267±6.748)%,P=0.011,28 d:(51.617±9.017)% vs (41.116±6.467)%,P=0.043],逃逸潜伏期时间[24 d:(62.967±5.494) s vs (63.917±7.027) s,P=0.800,25 d:(50.533±10.305)s vs (57.217±13.085) s,P=0.349,26 d:(40.333±10.526) s vs (50.133±11.039) s,P=0.147,27 d:(28.717±4.137) s vs (44.533±7.181) s,P=0.001,28 d:(21.950±6.889) s vs (37.567±5.974) s,P=0.002],炎症因子IL-1β和TNF-α表达量降低[IL-1β(42.690±3.057) ng/mL vs (750.167±51.941) ng/mL, TNF-α(71.670±4.996) ng/mL vs (1 085.167±68.535) ng/mL, P=0.000 6, 0.003)],神经营养因子BDNF和神经生长因子NGF的表达增加,损伤区域坏死细胞数显著降低[(54.833±5.486)个vs (74.000±5.323)个, P=0.031],成熟的神经元数量增多[(76.667±6.386)个vs (42.167±5.237)个, P=0.002],SGZ区新生细胞数增多[(12.667±0.760)个vs (7.500±1.258)个,P=0.031]。结论表儿茶酸促进脑损伤小鼠神经功能重塑,与抑制炎性反应、促进神经营养因子分泌和促神经再生密切相关。展开更多
Hypertension is a major problem worldwide. There is much evidence to suggest that reactive oxygen species (ROS) radical may play a role in the development of organ damage associated with cardiovascular disease and hyp...Hypertension is a major problem worldwide. There is much evidence to suggest that reactive oxygen species (ROS) radical may play a role in the development of organ damage associated with cardiovascular disease and hypertension. (-)Epicatechin, a member of tea catechins belonging to flavonoid group, is known to be a potent anti-oxidant. The study has been undertaken to evaluate the effect of (-)epicatechin on markers of oxidative stress: reduced glutathione (GSH) and membrane sulfhydryl (-SH) groups in erythrocytes from hypertensive patients. The effect of (-)epicatechin was also compared with a known anti-oxidant L-ascorbic acid. The erythrocyte intracellular GSH content and membrane -SH group content were significantly (P<0.01) decreased in hypertensive subjects. In vitro incubation with (-)epicatechin caused an increase in GSH and -SH content, the effect was more pronounced in hypertensive erythrocytes. Similar results were obtained with L-ascorbic acid. The observed decrease in the level of GSH and -SH groups in hypertension is an indicator of oxidative stress condition. Observation of an increase in red cell GSH content and the protection of membrane -SH group oxidation by (-)epicatechin in hypertensive subjects is a convincing reason to suggest that high dietary intake of foods rich in catechins may help to reduce oxidative stress and concomitant free radical damage in hypertensive patients.展开更多
Evicence qrcvided by epicemiological studies snovved that envionmenta tactorsucal agents play a mejox rbe gn tbe cawetgyn of the metyity of tri3 t(xnys the 'Tpesthat caxer rs twwt oo ewM d8ea3e me sedro odweto wan...Evicence qrcvided by epicemiological studies snovved that envionmenta tactorsucal agents play a mejox rbe gn tbe cawetgyn of the metyity of tri3 t(xnys the 'Tpesthat caxer rs twwt oo ewM d8ea3e me sedro odweto wanc5 tc amtarqever1tico sndd be m twrmt goa of cra raseach. betarv erecticec hgue bew1 ttro mp5'qMg aea tc expfore gu rrony varete5 of fregh vepetbes, fru15 an bev9op5 were展开更多
文摘目的探讨表儿茶酸[(-)-epicatechin,EC]对脑损伤(traumatic brain injury,TBI)小鼠的神经修复作用及机制。方法建立小鼠自由落体脑外伤动物模型,将50只C57BL/6小鼠随机(随机数字法)分为脑损伤+生理盐水(Veh)组,脑损伤+表儿茶酸(EC)组(EC组和Veh组各死亡1只,每组24只)。术后3 d,采用ELISA方法检测炎症因子IL-1β和TNF-ɑ的表达,PI染色检测分析损伤区坏死细胞数;水迷宫实验观察术后28 d小鼠空间学习记忆能力,qRT-PCR检测神经营养因子BDNF和NGF的表达,免疫荧光染色检测NeuN的表达,EdU染色观察SGZ区神经再生。结果与Veh组相比较,EC治疗组明显改善小鼠的空间学习记忆能力,平台象限停留时间增加[24 d:(26.333±5.037)% vs (26.583±5.802)%,P=0.938;25 d:(33.300±4.724)% vs (29.767±3.347)%,P=0.166;26 d:(41.017±7.246)% vs (32.800±8.145)%,P=0.095l;27 d:(48.017±7.424)% vs (35.267±6.748)%,P=0.011,28 d:(51.617±9.017)% vs (41.116±6.467)%,P=0.043],逃逸潜伏期时间[24 d:(62.967±5.494) s vs (63.917±7.027) s,P=0.800,25 d:(50.533±10.305)s vs (57.217±13.085) s,P=0.349,26 d:(40.333±10.526) s vs (50.133±11.039) s,P=0.147,27 d:(28.717±4.137) s vs (44.533±7.181) s,P=0.001,28 d:(21.950±6.889) s vs (37.567±5.974) s,P=0.002],炎症因子IL-1β和TNF-α表达量降低[IL-1β(42.690±3.057) ng/mL vs (750.167±51.941) ng/mL, TNF-α(71.670±4.996) ng/mL vs (1 085.167±68.535) ng/mL, P=0.000 6, 0.003)],神经营养因子BDNF和神经生长因子NGF的表达增加,损伤区域坏死细胞数显著降低[(54.833±5.486)个vs (74.000±5.323)个, P=0.031],成熟的神经元数量增多[(76.667±6.386)个vs (42.167±5.237)个, P=0.002],SGZ区新生细胞数增多[(12.667±0.760)个vs (7.500±1.258)个,P=0.031]。结论表儿茶酸促进脑损伤小鼠神经功能重塑,与抑制炎性反应、促进神经营养因子分泌和促神经再生密切相关。
文摘Hypertension is a major problem worldwide. There is much evidence to suggest that reactive oxygen species (ROS) radical may play a role in the development of organ damage associated with cardiovascular disease and hypertension. (-)Epicatechin, a member of tea catechins belonging to flavonoid group, is known to be a potent anti-oxidant. The study has been undertaken to evaluate the effect of (-)epicatechin on markers of oxidative stress: reduced glutathione (GSH) and membrane sulfhydryl (-SH) groups in erythrocytes from hypertensive patients. The effect of (-)epicatechin was also compared with a known anti-oxidant L-ascorbic acid. The erythrocyte intracellular GSH content and membrane -SH group content were significantly (P<0.01) decreased in hypertensive subjects. In vitro incubation with (-)epicatechin caused an increase in GSH and -SH content, the effect was more pronounced in hypertensive erythrocytes. Similar results were obtained with L-ascorbic acid. The observed decrease in the level of GSH and -SH groups in hypertension is an indicator of oxidative stress condition. Observation of an increase in red cell GSH content and the protection of membrane -SH group oxidation by (-)epicatechin in hypertensive subjects is a convincing reason to suggest that high dietary intake of foods rich in catechins may help to reduce oxidative stress and concomitant free radical damage in hypertensive patients.
文摘Evicence qrcvided by epicemiological studies snovved that envionmenta tactorsucal agents play a mejox rbe gn tbe cawetgyn of the metyity of tri3 t(xnys the 'Tpesthat caxer rs twwt oo ewM d8ea3e me sedro odweto wanc5 tc amtarqever1tico sndd be m twrmt goa of cra raseach. betarv erecticec hgue bew1 ttro mp5'qMg aea tc expfore gu rrony varete5 of fregh vepetbes, fru15 an bev9op5 were
