AIM：To in vestigate the relationship between the expression of inducible nitric oxide synthase(iNOS),vascular endothelial growth factor(VEGF),the microvascular density(MVD)and the pathological features and clinical s...AIM：To in vestigate the relationship between the expression of inducible nitric oxide synthase(iNOS),vascular endothelial growth factor(VEGF),the microvascular density(MVD)and the pathological features and clinical staging of gastric cancer.METHODS:Immunohistochemical staining was used for detecting the expression of iNOS and VEGFin46resected specimens of gastric carcinoma;the monoclonal antibody against CD34 was used for displaying vascular endothelial cells,and MVD was detected by counting of CD34-positive vascular endothelial cells.RESULTS:Of 46resected specimens of gastric carcinoma,the rates of expressions of iNOS and VEGF were 58.70%and76.09%,respectively,and MVDaveraged55.59±19.39,Judged by the standard TNM criteria,the rate of expression of iNOS in stageⅣ（84.46%)was higher than those in stageⅠ，Ⅱ，Ⅲ（Fish exact probabilities test,P=0.019,0.023and 0.033,respectively);the rates of expression of VEGFin stage Ⅲ，Ⅳ（76.0%,92.31%,respectively)were higher than those in stageⅠ，Ⅱ（Fis exact probabilities test,P=0.031,0.017,0.022and0.019).MVDs in stageⅢ，Ⅳ（64.72±14.96,67.09±18.29,respectively)were higher than those in stageⅠ，Ⅱ(t\2.378,4.015,2.503and2.450,P<0.05,P<0.001,P<0.001,P<0.05,respectively),In37gastric carcinoma specimens with lymph node metastasis,MVD(68.69±18.07)and the rates of expression of iNOS and VEGF(70.27%,83.78%,respectively)were higher than those in the specimens with absence of metastasis(t=2.205,X^2=6.3587,X^2=6.2584,P<0.01,P<0.05,P<0.05,respectively),MVD and the expressions of iNOS and EGF were not correlated to the location,size or grade of tumor,nor with the depth of invasion of tumor;MVDs in the positive iNOS and VEGF specimens(59.88±18.02,58.39±17.73,repectively)were higher than those in the negative iNOS and VEGF specimens(X^2=6.3587and 6.1574,P<0.05,P<0.05,respectively);thus the expressions of iNOS and VEGF was correlated to MVD,but the expression of iNOS was not correlated to that of VEGF,In addition.of the 46 surviving patients,the 5-year s展开更多
AIM: To observe the relationship between the expression of vascular endothelial growth factor (VEGF), microvascular density (MVD) and the pathological characteristics of esophageal and gastric carcinomas.METHODS: S-P ...AIM: To observe the relationship between the expression of vascular endothelial growth factor (VEGF), microvascular density (MVD) and the pathological characteristics of esophageal and gastric carcinomas.METHODS: S-P immunohistochemical staining was used to investigate the expression of VEGF in all the specimens.The antibody against factor VIII-related antigen was used to display vascular endothelial cells, and MVD was examined by counting the factor VIII-positive vascular endothelial cells.RESULTS: The positive rates of VEGF expression in esophageal carcinoma and gastric carcinoma were 81.36 %and 67.5 % respectively, and the MVD averaged 41.81±8.44and 34.36±9.67 respectively, which were higher than those in benign diseases. The expression of VEGF and MVD were closely correlated with the degree of differentiation, lymphatic metastasis, but not related to depth of cancer invasion. In early stage gastric carcinoma, the rate of expression of VEGF and MVD was lower than that in progressive gastric carcinomas. CONCLUSION: The expression of VEGF is correlated with tumor angiogenesis, and VEGF plays an important role in new blood vessels formation, the expression of VEGF and MVD play an important role in tumor growth and metastasis.MVD and the expression of VEGF may be two important indexes for patients′ prognosis.展开更多
AIM: The expression of vascular endothelial growth factor (VEGF) and its receptors KDR and Flt-1 by gastric carcinoma tissues and different gastric carcinoma cell lines was detected to elucidate the molecular mechanis...AIM: The expression of vascular endothelial growth factor (VEGF) and its receptors KDR and Flt-1 by gastric carcinoma tissues and different gastric carcinoma cell lines was detected to elucidate the molecular mechanism of this growth factor in promoting tumor growth.METHODS: The expression of VEGF, Flt-1 and KDR was determined by reverse transcription-polymerase chain reaction (RT-PCR) in gastric cancer cell lines RF-1, RF-48,AGS-1, NCI-N87, NCI-SNU-1, NCI-SNU-5, NCI-SNU-16 and KATO-Ⅲ. The expression of Flt-1 and KDR in paraffinembedded specimens of gastric cancer was determined by immunohistochemistry. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to assess the role of VEGF in tumor cell proliferation.RESULTS: All 8 gastric cancer cell lines analyzed expressed VEGF121 and VEGF16s and six of them expressed both Flt-1 and KDR, while cell line NCI-SNU-5 expressed Flt-1 only and cell line KATOⅢ expressed neither Flt-1 nor KDR. The gastric carcinoma tissues expressed Flt-1 and KDR widely,with the positive rate of expression of Flt-1 and KDR being 84.6 % and 70 % respectively. The exogenous VEGF stimulated the growth of KDR-positive cell lines NCI-N87 and AGS-1 in a dose-dependent manner but exhibited no effect on the growth of KDR-negative cell line NCI-N87.CONCLUSION: VEGF and its receptors KDR and Flt-1 were expressed widely in gastric carcinoma cells and the VEGF stimulated KDR-positive tumor cell growth directly. These results suggest that VEGF may play a role in promoting tumor growth and metastasis by participating in both paracrine and autocrine pathways.展开更多
AIM: To investigate the role of cyclooxygenase-2(COX-2)and vascular endothelial growth factor (VEGF) in the development of gastric carcinoma and correlation between expression of COX-2 and VEGF and clinicopathologic f...AIM: To investigate the role of cyclooxygenase-2(COX-2)and vascular endothelial growth factor (VEGF) in the development of gastric carcinoma and correlation between expression of COX-2 and VEGF and clinicopathologic features in tissues from patients with gastric carcinoma.METHODS: 281 patients with gastric carcinoma who underwent surgical resection between 1990 and 1999 at the First Affiliated Hospital, Anhui Medical University, PRC, were followed up. Expression of COX-2 and VEGF was investigated retrospectively in 232 gastric carcinoma tissues and 60noncancerous specimens by using immunohistochemistry.RESULTS: The 5-year survival rates of early gastric carcinoma (EGC) and advanced gastric carcinoma (AGC) were 93.4 %and 59.0 %, respectively. Survival time was highly correlated with lymph node metastasis, vascular invasion, depth of invasion and treatment with chemotherapy. Compared with paired noncancerous tissues, expression of COX-2 and VEGF and microvessel density (MVD) value in carcinoma tissue were significantly higher. The MVD value was much higher in COX-2-positive group and VEGF-positive group than that in COX-2-negative group and VEGF-negative group.Expression of COX-2 and VEGF, as well as MVD value were highly correlated with lymph node metastasis and vascular invasion. The 5-year survival rate of patients with expression of COX-2 or VEGF was significantly lower than that of patients without COX-2 or VEGF expression. Multivariate analysis revealed that VEGF overexpression, lymph node metastasis,COX-2 overexpression, depth of invasion and vascular invasion were all independent prognostic factors of gastric carcinoma.CONCLUSION: Overexpression of COX-2 and VEGF in patients with gastric carcinoma can enhance the possibility of invasion and metastasis, implicating a poor prognosis.They may serve as the fairly good prognostic factors to indicate biologic behaviors of gastric carcinoma.展开更多
目的观察肛痈促愈汤在促进肛周脓肿患者术后创面愈合的作用及对血管内皮因子的影响。方法选择2018年1月—2019年8月医院收治的肛周脓肿患者83例,依照治疗方案的不同分为观察组(42例)和对照组(41例)。两组患者均采用一次性肛周脓肿根治...目的观察肛痈促愈汤在促进肛周脓肿患者术后创面愈合的作用及对血管内皮因子的影响。方法选择2018年1月—2019年8月医院收治的肛周脓肿患者83例,依照治疗方案的不同分为观察组(42例)和对照组(41例)。两组患者均采用一次性肛周脓肿根治术治疗。对照组采用高猛酸钾坐浴,患者大便后清洗创面,观察组采用肛痈促愈汤辅助治疗,直至创面愈合。比较两组治疗疗效,两组患者术后不同时间点症状及体征评分;比较两组腐肉完全脱离时间、创面愈合时间和创面缩小率;比较血清VEGF、IL-1β、TNF-α和IL-6含量;比较两组不同时段肛门功能。结果观察组总有效率为90.48%(38/42),对照组治疗总有效率为73.17%(30/41),观察组治疗总有效率高于对照组(P<0.05);观察组术后7 d和术后14 d肛门疼痛、肛门周围组织水肿、分泌物和肉芽形态的评分低于对照组(P<0.05);观察组患者腐肉完全脱离时间、创面愈合时间和创面缩小率均低于对照组(P<0.05);观察组术后14 d VEGF水平高于对照组,炎性细胞因子IL-6、IL-8和TNF-α水平低于对照组(P<0.05);观察组术后1个月和术后3个月肛管静息压和肛管最大收缩压均高于对照组(P<0.05)。结论肛痈促愈汤可促进肛周脓肿患者一次性根治术后创面愈合和肉芽生长,提高血清VEGF水平,降低炎症反应,有效恢复肛门收缩功能,提高临床总有效率。展开更多
文摘AIM：To in vestigate the relationship between the expression of inducible nitric oxide synthase(iNOS),vascular endothelial growth factor(VEGF),the microvascular density(MVD)and the pathological features and clinical staging of gastric cancer.METHODS:Immunohistochemical staining was used for detecting the expression of iNOS and VEGFin46resected specimens of gastric carcinoma;the monoclonal antibody against CD34 was used for displaying vascular endothelial cells,and MVD was detected by counting of CD34-positive vascular endothelial cells.RESULTS:Of 46resected specimens of gastric carcinoma,the rates of expressions of iNOS and VEGF were 58.70%and76.09%,respectively,and MVDaveraged55.59±19.39,Judged by the standard TNM criteria,the rate of expression of iNOS in stageⅣ（84.46%)was higher than those in stageⅠ，Ⅱ，Ⅲ（Fish exact probabilities test,P=0.019,0.023and 0.033,respectively);the rates of expression of VEGFin stage Ⅲ，Ⅳ（76.0%,92.31%,respectively)were higher than those in stageⅠ，Ⅱ（Fis exact probabilities test,P=0.031,0.017,0.022and0.019).MVDs in stageⅢ，Ⅳ（64.72±14.96,67.09±18.29,respectively)were higher than those in stageⅠ，Ⅱ(t\2.378,4.015,2.503and2.450,P<0.05,P<0.001,P<0.001,P<0.05,respectively),In37gastric carcinoma specimens with lymph node metastasis,MVD(68.69±18.07)and the rates of expression of iNOS and VEGF(70.27%,83.78%,respectively)were higher than those in the specimens with absence of metastasis(t=2.205,X^2=6.3587,X^2=6.2584,P<0.01,P<0.05,P<0.05,respectively),MVD and the expressions of iNOS and EGF were not correlated to the location,size or grade of tumor,nor with the depth of invasion of tumor;MVDs in the positive iNOS and VEGF specimens(59.88±18.02,58.39±17.73,repectively)were higher than those in the negative iNOS and VEGF specimens(X^2=6.3587and 6.1574,P<0.05,P<0.05,respectively);thus the expressions of iNOS and VEGF was correlated to MVD,but the expression of iNOS was not correlated to that of VEGF,In addition.of the 46 surviving patients,the 5-year s
文摘AIM: To observe the relationship between the expression of vascular endothelial growth factor (VEGF), microvascular density (MVD) and the pathological characteristics of esophageal and gastric carcinomas.METHODS: S-P immunohistochemical staining was used to investigate the expression of VEGF in all the specimens.The antibody against factor VIII-related antigen was used to display vascular endothelial cells, and MVD was examined by counting the factor VIII-positive vascular endothelial cells.RESULTS: The positive rates of VEGF expression in esophageal carcinoma and gastric carcinoma were 81.36 %and 67.5 % respectively, and the MVD averaged 41.81±8.44and 34.36±9.67 respectively, which were higher than those in benign diseases. The expression of VEGF and MVD were closely correlated with the degree of differentiation, lymphatic metastasis, but not related to depth of cancer invasion. In early stage gastric carcinoma, the rate of expression of VEGF and MVD was lower than that in progressive gastric carcinomas. CONCLUSION: The expression of VEGF is correlated with tumor angiogenesis, and VEGF plays an important role in new blood vessels formation, the expression of VEGF and MVD play an important role in tumor growth and metastasis.MVD and the expression of VEGF may be two important indexes for patients′ prognosis.
基金National Nature Science Foundation for Outstanding Young Scientist of China (to S.CC.,No.39525021)National 863 program of China (2002 AA 216111)Beijing Laboratory of Cancer Molecular Biology.
文摘AIM: The expression of vascular endothelial growth factor (VEGF) and its receptors KDR and Flt-1 by gastric carcinoma tissues and different gastric carcinoma cell lines was detected to elucidate the molecular mechanism of this growth factor in promoting tumor growth.METHODS: The expression of VEGF, Flt-1 and KDR was determined by reverse transcription-polymerase chain reaction (RT-PCR) in gastric cancer cell lines RF-1, RF-48,AGS-1, NCI-N87, NCI-SNU-1, NCI-SNU-5, NCI-SNU-16 and KATO-Ⅲ. The expression of Flt-1 and KDR in paraffinembedded specimens of gastric cancer was determined by immunohistochemistry. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to assess the role of VEGF in tumor cell proliferation.RESULTS: All 8 gastric cancer cell lines analyzed expressed VEGF121 and VEGF16s and six of them expressed both Flt-1 and KDR, while cell line NCI-SNU-5 expressed Flt-1 only and cell line KATOⅢ expressed neither Flt-1 nor KDR. The gastric carcinoma tissues expressed Flt-1 and KDR widely,with the positive rate of expression of Flt-1 and KDR being 84.6 % and 70 % respectively. The exogenous VEGF stimulated the growth of KDR-positive cell lines NCI-N87 and AGS-1 in a dose-dependent manner but exhibited no effect on the growth of KDR-negative cell line NCI-N87.CONCLUSION: VEGF and its receptors KDR and Flt-1 were expressed widely in gastric carcinoma cells and the VEGF stimulated KDR-positive tumor cell growth directly. These results suggest that VEGF may play a role in promoting tumor growth and metastasis by participating in both paracrine and autocrine pathways.
文摘AIM: To investigate the role of cyclooxygenase-2(COX-2)and vascular endothelial growth factor (VEGF) in the development of gastric carcinoma and correlation between expression of COX-2 and VEGF and clinicopathologic features in tissues from patients with gastric carcinoma.METHODS: 281 patients with gastric carcinoma who underwent surgical resection between 1990 and 1999 at the First Affiliated Hospital, Anhui Medical University, PRC, were followed up. Expression of COX-2 and VEGF was investigated retrospectively in 232 gastric carcinoma tissues and 60noncancerous specimens by using immunohistochemistry.RESULTS: The 5-year survival rates of early gastric carcinoma (EGC) and advanced gastric carcinoma (AGC) were 93.4 %and 59.0 %, respectively. Survival time was highly correlated with lymph node metastasis, vascular invasion, depth of invasion and treatment with chemotherapy. Compared with paired noncancerous tissues, expression of COX-2 and VEGF and microvessel density (MVD) value in carcinoma tissue were significantly higher. The MVD value was much higher in COX-2-positive group and VEGF-positive group than that in COX-2-negative group and VEGF-negative group.Expression of COX-2 and VEGF, as well as MVD value were highly correlated with lymph node metastasis and vascular invasion. The 5-year survival rate of patients with expression of COX-2 or VEGF was significantly lower than that of patients without COX-2 or VEGF expression. Multivariate analysis revealed that VEGF overexpression, lymph node metastasis,COX-2 overexpression, depth of invasion and vascular invasion were all independent prognostic factors of gastric carcinoma.CONCLUSION: Overexpression of COX-2 and VEGF in patients with gastric carcinoma can enhance the possibility of invasion and metastasis, implicating a poor prognosis.They may serve as the fairly good prognostic factors to indicate biologic behaviors of gastric carcinoma.
文摘目的观察肛痈促愈汤在促进肛周脓肿患者术后创面愈合的作用及对血管内皮因子的影响。方法选择2018年1月—2019年8月医院收治的肛周脓肿患者83例,依照治疗方案的不同分为观察组(42例)和对照组(41例)。两组患者均采用一次性肛周脓肿根治术治疗。对照组采用高猛酸钾坐浴,患者大便后清洗创面,观察组采用肛痈促愈汤辅助治疗,直至创面愈合。比较两组治疗疗效,两组患者术后不同时间点症状及体征评分;比较两组腐肉完全脱离时间、创面愈合时间和创面缩小率;比较血清VEGF、IL-1β、TNF-α和IL-6含量;比较两组不同时段肛门功能。结果观察组总有效率为90.48%(38/42),对照组治疗总有效率为73.17%(30/41),观察组治疗总有效率高于对照组(P<0.05);观察组术后7 d和术后14 d肛门疼痛、肛门周围组织水肿、分泌物和肉芽形态的评分低于对照组(P<0.05);观察组患者腐肉完全脱离时间、创面愈合时间和创面缩小率均低于对照组(P<0.05);观察组术后14 d VEGF水平高于对照组,炎性细胞因子IL-6、IL-8和TNF-α水平低于对照组(P<0.05);观察组术后1个月和术后3个月肛管静息压和肛管最大收缩压均高于对照组(P<0.05)。结论肛痈促愈汤可促进肛周脓肿患者一次性根治术后创面愈合和肉芽生长,提高血清VEGF水平,降低炎症反应,有效恢复肛门收缩功能,提高临床总有效率。
