Based on nine morphological characters of populations of Tegillarca granosa from Guangxi, Zhejiang, Shandong,of China and Lishui, Korea, multivariate morphometrics was used to investigate their morphological variation...Based on nine morphological characters of populations of Tegillarca granosa from Guangxi, Zhejiang, Shandong,of China and Lishui, Korea, multivariate morphometrics was used to investigate their morphological variations among the four different geographical populations. ANOVA indicated that four populations showed significant morphological variations respectively (P <0.01). In the result of principal component analysis, two principal components (PC) were constructed by factor loading, in which the first principal component (PC1) was affected by shell width, shell height and shell moisture weight, and the PC2 was affected by hinge tooth, and the contributory ratios of two PC were 41.02% and 20.41%, respectively. The results of principal component analysis and cluster analysis revealed that the morphological chTegillraca granosa from Zhejiang population and Shandong population were similar. The discriminant functions of four populations were established, and the discriminant accuracy was 90.80%, 80.89%, 71.76%, and 97.46% respectively, so the average discriminant accuracy was 86.23%.展开更多
Mitochondrial gene(16S rRNA and COI) fragments of Bloody clam Scapharca broughtonii were amplified via PCR, the PCR products were ligated into T vectors, cloned and sequenced. 823 bp and 703 bp nucleotide sequences of...Mitochondrial gene(16S rRNA and COI) fragments of Bloody clam Scapharca broughtonii were amplified via PCR, the PCR products were ligated into T vectors, cloned and sequenced. 823 bp and 703 bp nucleotide sequences of partial 16S rRNA gene and partial COI gene were obtained respectively. The contents of A, T, G and C were 24.79%,23.57%,29.16% and 22.48% in 16S rDNA; 22.05%,33.85%,23.33% and 20.77% in COI. The potential uses of these two sequences were discussed for genetic variation, differentiation and relevant research of different geographic populations in the species.展开更多
文摘Based on nine morphological characters of populations of Tegillarca granosa from Guangxi, Zhejiang, Shandong,of China and Lishui, Korea, multivariate morphometrics was used to investigate their morphological variations among the four different geographical populations. ANOVA indicated that four populations showed significant morphological variations respectively (P <0.01). In the result of principal component analysis, two principal components (PC) were constructed by factor loading, in which the first principal component (PC1) was affected by shell width, shell height and shell moisture weight, and the PC2 was affected by hinge tooth, and the contributory ratios of two PC were 41.02% and 20.41%, respectively. The results of principal component analysis and cluster analysis revealed that the morphological chTegillraca granosa from Zhejiang population and Shandong population were similar. The discriminant functions of four populations were established, and the discriminant accuracy was 90.80%, 80.89%, 71.76%, and 97.46% respectively, so the average discriminant accuracy was 86.23%.
文摘Mitochondrial gene(16S rRNA and COI) fragments of Bloody clam Scapharca broughtonii were amplified via PCR, the PCR products were ligated into T vectors, cloned and sequenced. 823 bp and 703 bp nucleotide sequences of partial 16S rRNA gene and partial COI gene were obtained respectively. The contents of A, T, G and C were 24.79%,23.57%,29.16% and 22.48% in 16S rDNA; 22.05%,33.85%,23.33% and 20.77% in COI. The potential uses of these two sequences were discussed for genetic variation, differentiation and relevant research of different geographic populations in the species.
