目的从虎眼万年青中提取、分离水溶性多糖,初步研究其特征和抗肿瘤活性。方法采用热水提取,乙醇沉淀,Sevag法脱蛋白,DEAE-Sepharose Fast Flow离子交换柱色谱和Sephadex G-75凝胶过滤柱色谱分离纯化,得到虎眼万年青均一多糖OCAP-2-2。...目的从虎眼万年青中提取、分离水溶性多糖,初步研究其特征和抗肿瘤活性。方法采用热水提取,乙醇沉淀,Sevag法脱蛋白,DEAE-Sepharose Fast Flow离子交换柱色谱和Sephadex G-75凝胶过滤柱色谱分离纯化,得到虎眼万年青均一多糖OCAP-2-2。毛细管区带电泳法(CZE)分析单糖组成;采用高效凝胶渗透色谱法(HPGPC)测定多糖纯度和相对分子质量;动物移植性实体瘤的瘤重实验法研究对小鼠S180肉瘤的抑瘤作用,采用细胞体外培养技术,MTT法检测对人白血病细胞株K562细胞的增殖抑制作用。结果经过分离纯化得到的均一多糖组分OCAP-2-2主要由葡萄糖、木糖、甘露糖、半乳糖等4种单糖组成,其相对分子质量之比为2.16∶1.26:0.88∶1.00,平均相对分子质量9.84×104,总糖含量为92.3%,总糖醛酸含量为6.21%,蛋白质含量为3.68%;在0.1～100μg.mL-1内与荷瘤对照组相比,OCAP-2-2对小鼠S180肉瘤有显著的抑制活性,其中多糖浓度为100μg.mL-1时抑瘤率达(53.16±4.23)%(P<0.001);OCAP-2-2对K562细胞有明显的增殖抑制作用(P<0.01),在多糖浓度为0.10μg.mL-1时,增殖抑制率最高为(39.83±7.31)%(P<0.01)。结论 OCAP-2-2具有很高的抗肿瘤活性,可以探索作为一种潜在的天然抗肿瘤药物。展开更多
A new natural product (1) together with 26 know compounds were isolated from the Bulbs of Ornithogalum caudatum. Their structures were established on the basis of spectral analyses as n-butyl pyroglutamate (1), nonade...A new natural product (1) together with 26 know compounds were isolated from the Bulbs of Ornithogalum caudatum. Their structures were established on the basis of spectral analyses as n-butyl pyroglutamate (1), nonadecyl alcohol(2), eicosanol(3), behenic acid(4), b-sitosterol(5), stigmasterol(6), glycerol 1-monocerotate(7), pyrocatechol(8), p-ethoxybenzoic acid(9), p-coumarinic acid(10), protocatechuric acid(11), ursolic acid(12), betulinic acid(13), fumaric acid(14), succinic acid(15), uracil(16), xanthine(17), quercetin(18), kaempferol (19), isorham-netin(20), adenosine(21), daucosterol(22), stigmasterol 3-O-b-D-glucopyranoside(23), quercetin 3-O-b-D-glucopyra-noside(24), kaempferol 3-O-b-D-glucopyranoside(25), rutin(26), and kaempferol 3-O-b-rutinoside(27). All of them, except compound 5, were isolated from this plant for the first time.展开更多
目的探讨虎眼万年青总皂苷(Ornithogalum caudatum AIT saponins)诱导人乳腺癌MDA-MB-231细胞的凋亡的机制,为开发应用于治疗乳腺癌的药物提供实验依据。方法用MTT比色法检测虎眼万年青总皂苷对人乳腺癌MDA-MB-231细胞增殖的抑制作用;...目的探讨虎眼万年青总皂苷(Ornithogalum caudatum AIT saponins)诱导人乳腺癌MDA-MB-231细胞的凋亡的机制,为开发应用于治疗乳腺癌的药物提供实验依据。方法用MTT比色法检测虎眼万年青总皂苷对人乳腺癌MDA-MB-231细胞增殖的抑制作用;流式细胞术分析细胞凋亡;Western Blotting法检测细胞凋亡相关蛋白Bcl-2、Bax与Bad表达的变化。结果虎眼万年青总皂苷对人乳腺癌MDA-MB-231细胞生长有明显抑制作用,并呈剂量、时间依赖性;能诱导人乳腺癌MDA-MB-231细胞发生凋亡,且凋亡率呈浓度依赖性;能下调Bcl-2蛋白的表达,升高Bax/Bcl-2的比值,其高剂量组具有显著统计学差异(P<0.01),能上调Bad蛋白的表达,其低、中、高剂量组具有显著统计学差异(P<0.01)。结论虎眼万年青总皂苷可抑制乳腺癌MDA-MB-231细胞增殖,并可诱导细胞凋亡,其机制可能与升高Bax/Bcl-2的比值,上调Bad蛋白的表达相关。展开更多
文摘目的从虎眼万年青中提取、分离水溶性多糖,初步研究其特征和抗肿瘤活性。方法采用热水提取,乙醇沉淀,Sevag法脱蛋白,DEAE-Sepharose Fast Flow离子交换柱色谱和Sephadex G-75凝胶过滤柱色谱分离纯化,得到虎眼万年青均一多糖OCAP-2-2。毛细管区带电泳法(CZE)分析单糖组成;采用高效凝胶渗透色谱法(HPGPC)测定多糖纯度和相对分子质量;动物移植性实体瘤的瘤重实验法研究对小鼠S180肉瘤的抑瘤作用,采用细胞体外培养技术,MTT法检测对人白血病细胞株K562细胞的增殖抑制作用。结果经过分离纯化得到的均一多糖组分OCAP-2-2主要由葡萄糖、木糖、甘露糖、半乳糖等4种单糖组成,其相对分子质量之比为2.16∶1.26:0.88∶1.00,平均相对分子质量9.84×104,总糖含量为92.3%,总糖醛酸含量为6.21%,蛋白质含量为3.68%;在0.1～100μg.mL-1内与荷瘤对照组相比,OCAP-2-2对小鼠S180肉瘤有显著的抑制活性,其中多糖浓度为100μg.mL-1时抑瘤率达(53.16±4.23)%(P<0.001);OCAP-2-2对K562细胞有明显的增殖抑制作用(P<0.01),在多糖浓度为0.10μg.mL-1时,增殖抑制率最高为(39.83±7.31)%(P<0.01)。结论 OCAP-2-2具有很高的抗肿瘤活性,可以探索作为一种潜在的天然抗肿瘤药物。
基金This work was supported by the Ministry of Science and Technology of China (96-901-05-266).
文摘A new natural product (1) together with 26 know compounds were isolated from the Bulbs of Ornithogalum caudatum. Their structures were established on the basis of spectral analyses as n-butyl pyroglutamate (1), nonadecyl alcohol(2), eicosanol(3), behenic acid(4), b-sitosterol(5), stigmasterol(6), glycerol 1-monocerotate(7), pyrocatechol(8), p-ethoxybenzoic acid(9), p-coumarinic acid(10), protocatechuric acid(11), ursolic acid(12), betulinic acid(13), fumaric acid(14), succinic acid(15), uracil(16), xanthine(17), quercetin(18), kaempferol (19), isorham-netin(20), adenosine(21), daucosterol(22), stigmasterol 3-O-b-D-glucopyranoside(23), quercetin 3-O-b-D-glucopyra-noside(24), kaempferol 3-O-b-D-glucopyranoside(25), rutin(26), and kaempferol 3-O-b-rutinoside(27). All of them, except compound 5, were isolated from this plant for the first time.
文摘目的探讨虎眼万年青总皂苷(Ornithogalum caudatum AIT saponins)诱导人乳腺癌MDA-MB-231细胞的凋亡的机制,为开发应用于治疗乳腺癌的药物提供实验依据。方法用MTT比色法检测虎眼万年青总皂苷对人乳腺癌MDA-MB-231细胞增殖的抑制作用;流式细胞术分析细胞凋亡;Western Blotting法检测细胞凋亡相关蛋白Bcl-2、Bax与Bad表达的变化。结果虎眼万年青总皂苷对人乳腺癌MDA-MB-231细胞生长有明显抑制作用,并呈剂量、时间依赖性;能诱导人乳腺癌MDA-MB-231细胞发生凋亡,且凋亡率呈浓度依赖性;能下调Bcl-2蛋白的表达,升高Bax/Bcl-2的比值,其高剂量组具有显著统计学差异(P<0.01),能上调Bad蛋白的表达,其低、中、高剂量组具有显著统计学差异(P<0.01)。结论虎眼万年青总皂苷可抑制乳腺癌MDA-MB-231细胞增殖,并可诱导细胞凋亡,其机制可能与升高Bax/Bcl-2的比值,上调Bad蛋白的表达相关。
