本文对生物发光技术测定血中 ATP 含量的方法进行了探讨,并测定了24例正常人血浆中 ATP 含量。本方法的研究为临床医学中疾病的诊断、治疗提供了一个新指标。原理荧火虫尾灯内含有的荧光酶,在Mg<sup>2+</sup>和氧的参与下,...本文对生物发光技术测定血中 ATP 含量的方法进行了探讨,并测定了24例正常人血浆中 ATP 含量。本方法的研究为临床医学中疾病的诊断、治疗提供了一个新指标。原理荧火虫尾灯内含有的荧光酶,在Mg<sup>2+</sup>和氧的参与下,能活化底物荧光素和ATP,并与氧分子产生氧化反应,导致光的发射。其反应式为:E+LH<sub>2</sub>+ATP（?）E-LH<sub>2</sub>-AMP+PPi（1）E-LH<sub>2</sub>-AMP+O<sub>2</sub>→L=O+AMP+CO<sub>2</sub>+hu （2）式中 E 代表荧光酶,LH<sub>2</sub>为底物荧光素,PPi 为焦磷酸。在体外实验中,将生物体中的 ATP 抽出,加入荧光素与荧光酶的复合系统,它们便起专一性反应,迅速发出光量子。展开更多
Ubiquitination plays a crucial role in retinoic acid-inducible gene I(RIG-I)-induced antiviral responses.However, the precise regulatory mechanisms of RIG-I activity mediated by conjugated and unanchored ubiquitin cha...Ubiquitination plays a crucial role in retinoic acid-inducible gene I(RIG-I)-induced antiviral responses.However, the precise regulatory mechanisms of RIG-I activity mediated by conjugated and unanchored ubiquitin chains remain to be determined. In this study, we discovered that T55 of RIG-I was required for its binding ability for the unanchored ubiquitin chains. Experimental and mathematical analysis showed that unanchored ubiquitin chains associated with RIG-I were essential for sustained activation of type I interferon(IFN) signaling. Transcriptomics study revealed that the binding of RIG-I with unanchored ubiquitin chains additionally regulated the expression of a subset of metabolic and cell fate decision genes. Moreover, we found that ubiquitin-specific peptidase 21(USP21) and USP3 deubiquitinate conjugated and unanchored ubiquitin chains on RIG-I respectively. Taken together, characterization of the regulation mode and functions of conjugated ubiquitination and the unconjugated ubiquitin chainbinding of RIG-I may provide means to fine-tune RIG-I-mediated type I IFN signaling.展开更多
文摘本文对生物发光技术测定血中 ATP 含量的方法进行了探讨,并测定了24例正常人血浆中 ATP 含量。本方法的研究为临床医学中疾病的诊断、治疗提供了一个新指标。原理荧火虫尾灯内含有的荧光酶,在Mg<sup>2+</sup>和氧的参与下,能活化底物荧光素和ATP,并与氧分子产生氧化反应,导致光的发射。其反应式为:E+LH<sub>2</sub>+ATP（?）E-LH<sub>2</sub>-AMP+PPi（1）E-LH<sub>2</sub>-AMP+O<sub>2</sub>→L=O+AMP+CO<sub>2</sub>+hu （2）式中 E 代表荧光酶,LH<sub>2</sub>为底物荧光素,PPi 为焦磷酸。在体外实验中,将生物体中的 ATP 抽出,加入荧光素与荧光酶的复合系统,它们便起专一性反应,迅速发出光量子。
基金supported by the National Key Research and Development Program of China(2020YFA0908700)the National Natural Science Foundation of China(31870862,31700760,31770978,and 91742109)+4 种基金China Postdoctoral Science Foundation(2019M663225)Science and Technology Planning Project of GuangzhouChina(201804010385)Fundamental Research Funds for the Central Universities(18lgpy49)Guangdong Basic and Applied Basic Research Foundation(2019A1515110508)。
文摘Ubiquitination plays a crucial role in retinoic acid-inducible gene I(RIG-I)-induced antiviral responses.However, the precise regulatory mechanisms of RIG-I activity mediated by conjugated and unanchored ubiquitin chains remain to be determined. In this study, we discovered that T55 of RIG-I was required for its binding ability for the unanchored ubiquitin chains. Experimental and mathematical analysis showed that unanchored ubiquitin chains associated with RIG-I were essential for sustained activation of type I interferon(IFN) signaling. Transcriptomics study revealed that the binding of RIG-I with unanchored ubiquitin chains additionally regulated the expression of a subset of metabolic and cell fate decision genes. Moreover, we found that ubiquitin-specific peptidase 21(USP21) and USP3 deubiquitinate conjugated and unanchored ubiquitin chains on RIG-I respectively. Taken together, characterization of the regulation mode and functions of conjugated ubiquitination and the unconjugated ubiquitin chainbinding of RIG-I may provide means to fine-tune RIG-I-mediated type I IFN signaling.
