Apple scab caused by Venturia inaequalis has a tendency to spread and threatens the development of apple production in recent years in China.The genetic diversity and population structure were investigated by using si...Apple scab caused by Venturia inaequalis has a tendency to spread and threatens the development of apple production in recent years in China.The genetic diversity and population structure were investigated by using simple sequence repeat(SSR) markers.51 strains were classified into 3 groups by UPGMA method as Xunyi,Xingping and U.K.population,each of them mainly including strains from its original place.A relatively high level of genetic diversity was revealed: H=0.425 3,I=0.675 8,PPL=66.67%(at species level);H=0.149 1,I=0.228 0,PPL=44.44%(at population level).A high level of genetic differentiation was detected among/within populations with Nei’s Gst analysis and AMOVA.Molecular genetic variance within populations was greater than that among populations.Genetic variance among populations might result from barriers to gene flow(Nm=0.675 8).Genetic variance within populations might result from sexual pro-pagation of V.inaequalis.展开更多
采用电子显微镜技术,研究了苹果黑星病菌在苹果叶片上发育过程。扫描电子显微镜观察结果表明,接种后12 h 分生孢子即可萌发并形成附着胞,统计结果显示其孢子萌发率在6 h和12 h分别为83% 和95%,附着胞形成率在12 h和24 h 分别为93% 和95...采用电子显微镜技术,研究了苹果黑星病菌在苹果叶片上发育过程。扫描电子显微镜观察结果表明,接种后12 h 分生孢子即可萌发并形成附着胞,统计结果显示其孢子萌发率在6 h和12 h分别为83% 和95%,附着胞形成率在12 h和24 h 分别为93% 和95%。透射电子显微镜观察结果表明,黑星病菌侵入以后在寄主角质层下和表皮细胞之间扩展、定殖并可形成子座。接种后12d,病菌开始从子座上产生分生孢子梗和分生孢子,分生孢子梗顶端每产生一个单生的分生孢子就形成一个环痕并延伸其长度。分生孢子梗和分生孢子主要沿叶脉形成,在叶片上呈网状扩展,此时叶片表现明显的病害症状。展开更多
文摘Apple scab caused by Venturia inaequalis has a tendency to spread and threatens the development of apple production in recent years in China.The genetic diversity and population structure were investigated by using simple sequence repeat(SSR) markers.51 strains were classified into 3 groups by UPGMA method as Xunyi,Xingping and U.K.population,each of them mainly including strains from its original place.A relatively high level of genetic diversity was revealed: H=0.425 3,I=0.675 8,PPL=66.67%(at species level);H=0.149 1,I=0.228 0,PPL=44.44%(at population level).A high level of genetic differentiation was detected among/within populations with Nei’s Gst analysis and AMOVA.Molecular genetic variance within populations was greater than that among populations.Genetic variance among populations might result from barriers to gene flow(Nm=0.675 8).Genetic variance within populations might result from sexual pro-pagation of V.inaequalis.
基金Supported by Chun-Hui China-France co-operation Project of Education Ministry of China and partly by the Science Committee of Shaanxi province(99K06-G2-01)
文摘采用电子显微镜技术,研究了苹果黑星病菌在苹果叶片上发育过程。扫描电子显微镜观察结果表明,接种后12 h 分生孢子即可萌发并形成附着胞,统计结果显示其孢子萌发率在6 h和12 h分别为83% 和95%,附着胞形成率在12 h和24 h 分别为93% 和95%。透射电子显微镜观察结果表明,黑星病菌侵入以后在寄主角质层下和表皮细胞之间扩展、定殖并可形成子座。接种后12d,病菌开始从子座上产生分生孢子梗和分生孢子,分生孢子梗顶端每产生一个单生的分生孢子就形成一个环痕并延伸其长度。分生孢子梗和分生孢子主要沿叶脉形成,在叶片上呈网状扩展,此时叶片表现明显的病害症状。
