A three-compartments rhizobox was designed and used to study the low-molecular-weight organic acids in root exudates and the root apoplastic iron of "lime-induced chlorosis" peanut grown on a calcareous soil...A three-compartments rhizobox was designed and used to study the low-molecular-weight organic acids in root exudates and the root apoplastic iron of "lime-induced chlorosis" peanut grown on a calcareous soil in relation to different soil moisture conditions. Results showed that chlorosis of peanuts developed under condition of high soil moisture level (250 g kg-1), while peanuts grew well and chlorosis did not develop when soil moisture was managed to a normal level (150 g kg-1). The malic acid, maleic acid and succinic acid contents of chlorotic peanut increased by 108.723, 0.029 and 22.446 ig cm-2, respectively, compared with healthy peanuts. The content of citric acid and fumaric acid also increased in root exudates of chlorotic peanuts. On Days 28 and 42 of peanut growth, the accumulation of root apoplastic iron in chlorotic peanuts was higher than that of healthy peanuts. From Day 28 to Day 42, the mobilization percentages of chlorotic peanuts and healthy peanuts to root apoplastic iron were almost the same, being 52.4% and 52.8%, respectively, indicating that the chlorosis might be caused by the inactivation of iron within peanut plant grown on a calcareous soil under high soil moisture conditions.展开更多
目的： 建立鸡血藤药材中缩合鞣质的含量测定方法，并对不同批次鸡血藤药材及其不同部位中缩合鞣质的含量进行测定。方法： 优化并建立了采用香草醛-硫酸显色体系测定鸡血藤药材中缩合鞣质含量的方法，反应条件为3 mL 3%香草醛，3 mL 40...目的： 建立鸡血藤药材中缩合鞣质的含量测定方法，并对不同批次鸡血藤药材及其不同部位中缩合鞣质的含量进行测定。方法： 优化并建立了采用香草醛-硫酸显色体系测定鸡血藤药材中缩合鞣质含量的方法，反应条件为3 mL 3%香草醛，3 mL 40%浓硫酸，常温反应20 min，测定波长为500 nm。结果： 5个批次鸡血藤药材中缩合鞣质含量为3.99%-8.63%；其中3个批次药材不同部位的缩合鞣质含量测定结果显示，树脂状分泌物中含量最高，为8.14%-11.51%，木质部（5.39%-7.92%）和韧皮部（3.65%-6.90%）含量相对较少。结论： 香草醛-硫酸显色法快速、简便、准确、重复性好， 可用于测定鸡血藤药材中缩合鞣质的含量；缩合鞣质在树脂状分泌物中的含量比其他部位高，为传统的鸡血藤药材质量的判断方法提供了科学依据。展开更多
基金Project (No. 39790100) supported by the National Natural Science Foundation of China.
文摘A three-compartments rhizobox was designed and used to study the low-molecular-weight organic acids in root exudates and the root apoplastic iron of "lime-induced chlorosis" peanut grown on a calcareous soil in relation to different soil moisture conditions. Results showed that chlorosis of peanuts developed under condition of high soil moisture level (250 g kg-1), while peanuts grew well and chlorosis did not develop when soil moisture was managed to a normal level (150 g kg-1). The malic acid, maleic acid and succinic acid contents of chlorotic peanut increased by 108.723, 0.029 and 22.446 ig cm-2, respectively, compared with healthy peanuts. The content of citric acid and fumaric acid also increased in root exudates of chlorotic peanuts. On Days 28 and 42 of peanut growth, the accumulation of root apoplastic iron in chlorotic peanuts was higher than that of healthy peanuts. From Day 28 to Day 42, the mobilization percentages of chlorotic peanuts and healthy peanuts to root apoplastic iron were almost the same, being 52.4% and 52.8%, respectively, indicating that the chlorosis might be caused by the inactivation of iron within peanut plant grown on a calcareous soil under high soil moisture conditions.
