Objective：Aroma is the core factor in aromatherapy. Sensory evaluation of aromas differed among three sweet osmanthus （Osmanthus fragrans） cultivar groups. The purpose of this study was to investigate the aroma-act...Objective：Aroma is the core factor in aromatherapy. Sensory evaluation of aromas differed among three sweet osmanthus （Osmanthus fragrans） cultivar groups. The purpose of this study was to investigate the aroma-active compounds responsible for these differences. Methods： Gas chromatography-olfactometry （GC-O） and GC-mass spectrometry （GC-MS） were used to analyze the aroma-active compounds and volatiles of creamy-white （‘Houban Yingui’, HBYG）, yellow （‘Liuye Jingui’, LYJG）, and orange （‘Gecheng Dangui’, GCDG） cultivars. Results：Seventeen aroma-active compounds were detected among 54 volatiles. trans-β-Ocimene, trans-β-ionone, and linalool, which were major volatiles, were identified as aroma-active, while cis-3-hexenyl butanoate,γ-terpinene, and hexyl butanoate were also aroma-active compounds, although their contents were low. Analysis of the odors was based on the sum of the modified frequency （MF） values of aroma-active compounds in different odor groups. HBYG contained more herb odors, contributed by cis-β-ocimene and trans-β-ocimene, while LYJG had more woody/violet/fruity odors released by trans-β-ionone, α-ionone, and hexyl butanoate. In GCDG, the more floral odors were the result of cis-linalool oxide, trans-linalool oxide, and linalool. Conclusions：Aroma-active compounds were not necessarily only the major volatiles：some volatiles with low content also contributed to aroma. The aroma differences among the three cultivars resulted from variation in the content of different odor groups and in the intensities of aroma-active compounds.展开更多
运用顶空固相微萃取、气相色谱-质谱联用、气相色谱-嗅闻(gas chromatography-olfactometry,GC-O)技术,结合气味活性值(odor active value,OAV)对菇娘果挥发性成分和香气活性成分进行鉴定,综合评价单个挥发性组分对整体香气贡献程度,确...运用顶空固相微萃取、气相色谱-质谱联用、气相色谱-嗅闻(gas chromatography-olfactometry,GC-O)技术,结合气味活性值(odor active value,OAV)对菇娘果挥发性成分和香气活性成分进行鉴定,综合评价单个挥发性组分对整体香气贡献程度,确定关键香气成分。结果表明,菇娘果中有43种挥发性组分,其中有10种主要香气活性物质(OAV>1),主要包括4种醛酮类化合物(正己醛、甲硫基丙醛、反-2-顺-6-壬二烯醛、1-辛烯-3-酮),其香气分别描述为青草味、薯片味、黄瓜味、蘑菇味;5种酯类化合物(甲基-2-甲基丙酸酯、丁酸甲酯、2-甲基丁酸甲酯、丁酸甲基乙基酯、癸酸甲酯),其香气分别描述为花香、果香、苹果香、刺激果香、葡萄酒香;1种呋喃类化合物(2-戊基呋喃),其香气描述为焦糖味。GC-O法中,4名嗅闻人员主要鉴定出“苹果味”2-甲基丁酸甲酯、“薯片味”甲硫基丙醛和“青草味”己醛。通过香气定性和感官定量描述性分析发现,2-甲基丁酸甲酯、甲硫基丙醛和己醛是菇娘果的主要香气成分。展开更多
基金Project supported by the PhD Program Foundation of the Ministry of Education of China(No.20130146110022)the National Natural Science Foundation of China(No.31070623)
文摘Objective：Aroma is the core factor in aromatherapy. Sensory evaluation of aromas differed among three sweet osmanthus （Osmanthus fragrans） cultivar groups. The purpose of this study was to investigate the aroma-active compounds responsible for these differences. Methods： Gas chromatography-olfactometry （GC-O） and GC-mass spectrometry （GC-MS） were used to analyze the aroma-active compounds and volatiles of creamy-white （‘Houban Yingui’, HBYG）, yellow （‘Liuye Jingui’, LYJG）, and orange （‘Gecheng Dangui’, GCDG） cultivars. Results：Seventeen aroma-active compounds were detected among 54 volatiles. trans-β-Ocimene, trans-β-ionone, and linalool, which were major volatiles, were identified as aroma-active, while cis-3-hexenyl butanoate,γ-terpinene, and hexyl butanoate were also aroma-active compounds, although their contents were low. Analysis of the odors was based on the sum of the modified frequency （MF） values of aroma-active compounds in different odor groups. HBYG contained more herb odors, contributed by cis-β-ocimene and trans-β-ocimene, while LYJG had more woody/violet/fruity odors released by trans-β-ionone, α-ionone, and hexyl butanoate. In GCDG, the more floral odors were the result of cis-linalool oxide, trans-linalool oxide, and linalool. Conclusions：Aroma-active compounds were not necessarily only the major volatiles：some volatiles with low content also contributed to aroma. The aroma differences among the three cultivars resulted from variation in the content of different odor groups and in the intensities of aroma-active compounds.
文摘运用顶空固相微萃取、气相色谱-质谱联用、气相色谱-嗅闻(gas chromatography-olfactometry,GC-O)技术,结合气味活性值(odor active value,OAV)对菇娘果挥发性成分和香气活性成分进行鉴定,综合评价单个挥发性组分对整体香气贡献程度,确定关键香气成分。结果表明,菇娘果中有43种挥发性组分,其中有10种主要香气活性物质(OAV>1),主要包括4种醛酮类化合物(正己醛、甲硫基丙醛、反-2-顺-6-壬二烯醛、1-辛烯-3-酮),其香气分别描述为青草味、薯片味、黄瓜味、蘑菇味;5种酯类化合物(甲基-2-甲基丙酸酯、丁酸甲酯、2-甲基丁酸甲酯、丁酸甲基乙基酯、癸酸甲酯),其香气分别描述为花香、果香、苹果香、刺激果香、葡萄酒香;1种呋喃类化合物(2-戊基呋喃),其香气描述为焦糖味。GC-O法中,4名嗅闻人员主要鉴定出“苹果味”2-甲基丁酸甲酯、“薯片味”甲硫基丙醛和“青草味”己醛。通过香气定性和感官定量描述性分析发现,2-甲基丁酸甲酯、甲硫基丙醛和己醛是菇娘果的主要香气成分。
