A new model of pancreatic β-cell membrane chromatography has been established by using a β-cell membrane stationary phase (β-CMSP) prepared by immobi-lizing the β-cell membrane onto the surface of silica carrier. ...A new model of pancreatic β-cell membrane chromatography has been established by using a β-cell membrane stationary phase (β-CMSP) prepared by immobi-lizing the β-cell membrane onto the surface of silica carrier. The protein level and K+, Na+-ATP enzymatic activity of the β-CMSP were detected respectively. The surface characteris-tics of the β-CMSP were tested by using the scanning elec-tron microscope and surface energy spectrometer. In this model, the column (10 mm × 2 mm, I.D.) packed with β-CMSP, 25 mmo1/L ammonium sulfate buffer solution (pH 7.4) as mobile phase with the flow rate of 0.2 mL/min at 37 ± 0.5℃ were used in the following studies. The retention char-acteristics of the sulfonylureas (gliquidone, glibenclamide, gliclazide and glipizide) were investigated under the chro-matographic conditions above. The affinities of the sulfony-lureas on β-cell membrane and receptor will be expressed by using the logk′ values (the logarithm of capacity factor of a solute) in the model. The correlation of the affinity with the pharmacological effect of the sulfonylureas was analyzed also.展开更多
A new molecular representation, molecular hologram, is employed to investigate the quantitative rela- tionships between gas chromatographic retention indices (GC-RI) and molecular structures of polychlorinated diben- ...A new molecular representation, molecular hologram, is employed to investigate the quantitative rela- tionships between gas chromatographic retention indices (GC-RI) and molecular structures of polychlorinated diben- zofurans (PCDFs). Together with application of partial least squares (PLS) regression, the quantitative structure-reten- tion relationship (QSRR) model is constructed for GC-RI of 135 PCDFs. This new QSRR model presents high statistical quality and predictive value with crossvalidation correlation coefficient q2LOO values of 0.998, and non-crossvalidation correlation coefficient r2 of 0.998. 100 PCDFs are selected randomly as training set and the rest as testing set. The re- sult of PLS regressive analysis of training set yields r2 of 0.998 and q2LOO of 0.997. The GC-RIs of testing set are pre- dicted, and the correlation equation indicates that the model based on training set has excellent ability to predict the GC-RIs of PCDFs in testing set.展开更多
基金supported by the Shaanxi Natural Science Foundation(Grant No.2003C205,2002G231).
文摘A new model of pancreatic β-cell membrane chromatography has been established by using a β-cell membrane stationary phase (β-CMSP) prepared by immobi-lizing the β-cell membrane onto the surface of silica carrier. The protein level and K+, Na+-ATP enzymatic activity of the β-CMSP were detected respectively. The surface characteris-tics of the β-CMSP were tested by using the scanning elec-tron microscope and surface energy spectrometer. In this model, the column (10 mm × 2 mm, I.D.) packed with β-CMSP, 25 mmo1/L ammonium sulfate buffer solution (pH 7.4) as mobile phase with the flow rate of 0.2 mL/min at 37 ± 0.5℃ were used in the following studies. The retention char-acteristics of the sulfonylureas (gliquidone, glibenclamide, gliclazide and glipizide) were investigated under the chro-matographic conditions above. The affinities of the sulfony-lureas on β-cell membrane and receptor will be expressed by using the logk′ values (the logarithm of capacity factor of a solute) in the model. The correlation of the affinity with the pharmacological effect of the sulfonylureas was analyzed also.
基金This work was supported by the National Basic Research Program of China(Grant No.2003CB4 l5001)the National Natural Science Foundation of China(Grant No.20477018)+1 种基金European Commission International Scientific Cooperation Project(Grant No.ICA4-CT-2001-10039)the Doctoral Program of Higher Education of China and the Core University Program.
文摘A new molecular representation, molecular hologram, is employed to investigate the quantitative rela- tionships between gas chromatographic retention indices (GC-RI) and molecular structures of polychlorinated diben- zofurans (PCDFs). Together with application of partial least squares (PLS) regression, the quantitative structure-reten- tion relationship (QSRR) model is constructed for GC-RI of 135 PCDFs. This new QSRR model presents high statistical quality and predictive value with crossvalidation correlation coefficient q2LOO values of 0.998, and non-crossvalidation correlation coefficient r2 of 0.998. 100 PCDFs are selected randomly as training set and the rest as testing set. The re- sult of PLS regressive analysis of training set yields r2 of 0.998 and q2LOO of 0.997. The GC-RIs of testing set are pre- dicted, and the correlation equation indicates that the model based on training set has excellent ability to predict the GC-RIs of PCDFs in testing set.
