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基于丝素蛋白的可注射水凝胶在肌肉再生工程的应用 被引量:1
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作者 李艾元 施心雨 +3 位作者 刘梦斐 李诚 宣智宏 岳万福 《丝绸》 CAS CSCD 北大核心 2023年第2期42-48,共7页
针对丝素蛋白水凝胶可注射性较差、机械性能不稳定、无法植入细胞等问题,文章制作了一种新型的可注射丝素蛋白水凝胶。通过弱碱法脱去蚕丝的丝胶蛋白,经过溶解、透析、离心、过滤后获得丝素蛋白溶液,利用磷脂诱导丝素蛋白溶液形成水凝... 针对丝素蛋白水凝胶可注射性较差、机械性能不稳定、无法植入细胞等问题,文章制作了一种新型的可注射丝素蛋白水凝胶。通过弱碱法脱去蚕丝的丝胶蛋白,经过溶解、透析、离心、过滤后获得丝素蛋白溶液,利用磷脂诱导丝素蛋白溶液形成水凝胶。将水凝胶和细胞通过注射的方式植入湖羊耳部,分别在1、6、11 d取出水凝胶—肌肉细胞生长样品,通过Masson染色研究了水凝胶的降解规律和肌肉再生的过程。结果表明:这种基于丝素蛋白的新型水凝胶凝胶时间可控,制备简单,具有可注射性。水凝胶可以植入细胞,并且细胞在水凝胶中形成新的肌肉组织,说明这种基于丝素蛋白的新型水凝胶可以用作肌肉再生工程。进一步梳理丝素蛋白水凝胶的制备过程和切片结果发现:水凝胶制作简单,细胞在水凝胶中可以增殖分化,只需要注射就可以进入动物体内,在动物体内还可以降解。最终结果表明:基于丝素蛋白的水凝胶可以用肌肉组织再生工程,磷脂诱导的方式使水凝胶具备可注射性。 展开更多
关键词 水凝胶 凝结 磷脂 丝素蛋白 Β折叠
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Fuzzy Based Auto-coagulation Control Through Photometric Dispersion Analyzer
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作者 白桦 李圭白 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2004年第2期164-167,共4页
The main role of water treatment plants is to supply high-quality safe drinking water. Coagulation is one of the most important stages of surface water treatment. The photometric dispersion analyzer(PDA) is a new opti... The main role of water treatment plants is to supply high-quality safe drinking water. Coagulation is one of the most important stages of surface water treatment. The photometric dispersion analyzer(PDA) is a new optical method for flocculation monitoring, and is feasible to realize coagulation feedback control. The on line modification of the coagulation control system' s set point( or optimum dosing coagulant) has influenced the application of this technology in water treatment plant for a long time. A fuzzy control system incorporating the photometric dispersion analyzer was utilized in this coagulation control system. Proposed is a fuzzy logic inference control system by using Takagi and Sugeno' s fuzzy if-then rule for the self-correction of set point on line. Programmed is the dosing rate fuzzy control system in SIEMENS small-scale programmable logic controller. A 400 L/min middle-scale water treatment plant was utilized to simulate the reaction. With the changes of raw water quality, the set point was modified correctly in time, as well as coagulant dosing rate, and residual turbility before filtration was eligible and stable. Results show that this fuzzy inference and control system performs well on the coagulation control system through PDA. 展开更多
关键词 COAGULATION dosing rate control photometric dispersion analyzer fuzzy control fuzzy logic inference OPTIMUM water treatment
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