目的:通过网状Meta分析,评价四种两性霉素B静脉剂型在治疗真菌感染方面的疗效和安全性。方法:检索中英文电子数据库(PubMed、Scopus、Web of Science、the Cochrane Library、Embase、中国知网、万方数据库、维普数据库和中国生物医学...目的:通过网状Meta分析,评价四种两性霉素B静脉剂型在治疗真菌感染方面的疗效和安全性。方法:检索中英文电子数据库(PubMed、Scopus、Web of Science、the Cochrane Library、Embase、中国知网、万方数据库、维普数据库和中国生物医学文献数据库),纳入比较两性霉素B与三唑类或棘白菌素类药物治疗真菌感染的随机对照试验(RCT)进行网状Meta分析。检索时间为建库至2023年8月5日,文献质量采用GRADE分级。结果:共纳入16篇文献,涉及4365例患者。在疗效方面,两性霉素B脂质体>两性霉素B脱氧胆酸盐>两性霉素B胶体分散体,差异有统计学意义(P<0.05),两性霉素B脂质体复合物与其他剂型相比差异暂无统计学意义(P>0.05);在总不良反应发生率方面,四种两性霉素B静脉剂型的差异无统计学意义(P>0.05)。亚组分析结果显示,两性霉素B脱氧胆酸盐相比两性霉素B胶体分散体具有更高的神经系统毒性,差异有统计学意义(P<0.05)。结论:四种两性霉素B静脉剂型中,脂质体剂型的疗效优于脱氧胆酸盐传统剂型;胶体分散体剂型的疗效显著低于传统剂型,同时神经系统毒性也较低。由于研究纳入文献数量有限,可能存在发表偏倚,故仍需更多大样本量、高质量的临床研究进一步证实本研究的结果和结论。展开更多
It was recently suggested that the periodic and fractal precipitaion of calcium salts and proteins in cholesterol gallstonesis closely related to the process of stone formation.The present study is to mimic the proces...It was recently suggested that the periodic and fractal precipitaion of calcium salts and proteins in cholesterol gallstonesis closely related to the process of stone formation.The present study is to mimic the process of gallstone growth in vitro.We demonstrate for the first time that a three-dimensional structure of periodic rings (Liesegang ring)and/or fractal patterns can be produced by metal ion and deoxycholate precipitaiton which are similar to gallstones.Precipitation in a periodic pattern occurs more often than in a fractal structure,Using X-ray diffraction.polarized light microscope,Fourier transform-infrared(FT-IR) ,and extended X-ray absorption fine structure(EXAFS),we studied the pattern formation mechanisms of both types of structure .The results indicate that the rate of nucleation in the crystal growth is a crucial element of affect the pattern formation.The pattern selection between periodic and fractal type depends on the crystal growth speed and the rate of nucleation.展开更多
Background: Lipomas are benign neoplasms of mature fat cells. Current treatments are invasive and carry the risk of scarring. Injections of phosphatidylcholine solubilized with deoxycholate, a bile salt, have been use...Background: Lipomas are benign neoplasms of mature fat cells. Current treatments are invasive and carry the risk of scarring. Injections of phosphatidylcholine solubilized with deoxycholate, a bile salt, have been used to reduce unwanted accumulations of fat. Recent in vitro and ex vivo investigations indicate that deoxycholate alone causes adipocyte lysis. Objective: We sought to report our experience treating lipomas using subcutaneous deoxycholate injections. Methods: A total of 6 patients presenting with 12 lipomas were treated with intralesional injections of sodium deoxycholate (1.0% , 2.5% , and 5.0% ) at intervals of 2 to 20weeks. Tumor size, cutaneous reactions, and patients subjective responses were recorded before and after treatment. Results: All lipomas decreased in size (mean area reduction, 75% ; range, 37% - 100% ) as determined by clinical measurement (with ultrasound confirmation in one lipoma) after an average of 2.2 treatments. Several lipomas fragmented or became softer in addition to decreasing in volume. Adverse effects, including transient burning, erythema, and local swelling, were associated with higher deoxycholate concentrations but resolved without intervention. There was no clear association between deoxycholate concentration and efficacy. Conclusions: Our clinical experience supports our laboratory investigations demonstrating that deoxycholate, rather than phosphatidylcholine, is the active ingredient in subcutaneously injected formulas used to treat adipose tissue. This small series suggests that low concentration deoxycholate may be a relatively safe and effective treatment for small collections of fat. However, controlled clinical trials will be necessary to substantiate these observations.展开更多
本文从转录水平上研究了四种胆盐水解酶基因在植物乳杆菌KLDS1.0386中不同生长阶段的表达情况,并且分别以不同浓度的牛磺脱氧胆酸盐和甘氨脱氧胆酸盐为底物,以16s r RNA作为内参基因,利用实时荧光定量PCR技术研究不同浓度底物下的胆盐...本文从转录水平上研究了四种胆盐水解酶基因在植物乳杆菌KLDS1.0386中不同生长阶段的表达情况,并且分别以不同浓度的牛磺脱氧胆酸盐和甘氨脱氧胆酸盐为底物,以16s r RNA作为内参基因,利用实时荧光定量PCR技术研究不同浓度底物下的胆盐水解酶基因（bsh1、bsh2、bsh3、bsh4）表达的变化规律。结果显示,内参基因和目的基因的扩增效率均在90%~105%之间,说明目的基因的表达量可以被很好的反应出来。通过实时荧光定量PCR结果分析可知,植物乳杆菌KLDS1.0386中的4种胆盐水解酶基因随着生长时间的延长,表达量显著增加,而且在两种胆盐中都表达,基因表达量都被不同剂量（1%、2%、3%）的胆盐上调,上升幅度随着胆盐浓度的增加表现出极显著的增加趋势,而且胆盐不同,同浓度时每个基因的表达量也各不相同,在甘氨脱氧胆盐中的表达量普遍高于牛磺脱氧胆盐。展开更多
文摘目的:通过网状Meta分析,评价四种两性霉素B静脉剂型在治疗真菌感染方面的疗效和安全性。方法:检索中英文电子数据库(PubMed、Scopus、Web of Science、the Cochrane Library、Embase、中国知网、万方数据库、维普数据库和中国生物医学文献数据库),纳入比较两性霉素B与三唑类或棘白菌素类药物治疗真菌感染的随机对照试验(RCT)进行网状Meta分析。检索时间为建库至2023年8月5日,文献质量采用GRADE分级。结果:共纳入16篇文献,涉及4365例患者。在疗效方面,两性霉素B脂质体>两性霉素B脱氧胆酸盐>两性霉素B胶体分散体,差异有统计学意义(P<0.05),两性霉素B脂质体复合物与其他剂型相比差异暂无统计学意义(P>0.05);在总不良反应发生率方面,四种两性霉素B静脉剂型的差异无统计学意义(P>0.05)。亚组分析结果显示,两性霉素B脱氧胆酸盐相比两性霉素B胶体分散体具有更高的神经系统毒性,差异有统计学意义(P<0.05)。结论:四种两性霉素B静脉剂型中,脂质体剂型的疗效优于脱氧胆酸盐传统剂型;胶体分散体剂型的疗效显著低于传统剂型,同时神经系统毒性也较低。由于研究纳入文献数量有限,可能存在发表偏倚,故仍需更多大样本量、高质量的临床研究进一步证实本研究的结果和结论。
文摘It was recently suggested that the periodic and fractal precipitaion of calcium salts and proteins in cholesterol gallstonesis closely related to the process of stone formation.The present study is to mimic the process of gallstone growth in vitro.We demonstrate for the first time that a three-dimensional structure of periodic rings (Liesegang ring)and/or fractal patterns can be produced by metal ion and deoxycholate precipitaiton which are similar to gallstones.Precipitation in a periodic pattern occurs more often than in a fractal structure,Using X-ray diffraction.polarized light microscope,Fourier transform-infrared(FT-IR) ,and extended X-ray absorption fine structure(EXAFS),we studied the pattern formation mechanisms of both types of structure .The results indicate that the rate of nucleation in the crystal growth is a crucial element of affect the pattern formation.The pattern selection between periodic and fractal type depends on the crystal growth speed and the rate of nucleation.
文摘Background: Lipomas are benign neoplasms of mature fat cells. Current treatments are invasive and carry the risk of scarring. Injections of phosphatidylcholine solubilized with deoxycholate, a bile salt, have been used to reduce unwanted accumulations of fat. Recent in vitro and ex vivo investigations indicate that deoxycholate alone causes adipocyte lysis. Objective: We sought to report our experience treating lipomas using subcutaneous deoxycholate injections. Methods: A total of 6 patients presenting with 12 lipomas were treated with intralesional injections of sodium deoxycholate (1.0% , 2.5% , and 5.0% ) at intervals of 2 to 20weeks. Tumor size, cutaneous reactions, and patients subjective responses were recorded before and after treatment. Results: All lipomas decreased in size (mean area reduction, 75% ; range, 37% - 100% ) as determined by clinical measurement (with ultrasound confirmation in one lipoma) after an average of 2.2 treatments. Several lipomas fragmented or became softer in addition to decreasing in volume. Adverse effects, including transient burning, erythema, and local swelling, were associated with higher deoxycholate concentrations but resolved without intervention. There was no clear association between deoxycholate concentration and efficacy. Conclusions: Our clinical experience supports our laboratory investigations demonstrating that deoxycholate, rather than phosphatidylcholine, is the active ingredient in subcutaneously injected formulas used to treat adipose tissue. This small series suggests that low concentration deoxycholate may be a relatively safe and effective treatment for small collections of fat. However, controlled clinical trials will be necessary to substantiate these observations.
文摘本文从转录水平上研究了四种胆盐水解酶基因在植物乳杆菌KLDS1.0386中不同生长阶段的表达情况,并且分别以不同浓度的牛磺脱氧胆酸盐和甘氨脱氧胆酸盐为底物,以16s r RNA作为内参基因,利用实时荧光定量PCR技术研究不同浓度底物下的胆盐水解酶基因（bsh1、bsh2、bsh3、bsh4）表达的变化规律。结果显示,内参基因和目的基因的扩增效率均在90%~105%之间,说明目的基因的表达量可以被很好的反应出来。通过实时荧光定量PCR结果分析可知,植物乳杆菌KLDS1.0386中的4种胆盐水解酶基因随着生长时间的延长,表达量显著增加,而且在两种胆盐中都表达,基因表达量都被不同剂量（1%、2%、3%）的胆盐上调,上升幅度随着胆盐浓度的增加表现出极显著的增加趋势,而且胆盐不同,同浓度时每个基因的表达量也各不相同,在甘氨脱氧胆盐中的表达量普遍高于牛磺脱氧胆盐。
