AIM: To investigate the prognostic role of isoform 165 vascular endothelial growth factor messenger RNA (VEGF165 mRNA)in noncancerous liver tissues from patients with primary hepatocellular carcinoma (HCC).METHODS: Us...AIM: To investigate the prognostic role of isoform 165 vascular endothelial growth factor messenger RNA (VEGF165 mRNA)in noncancerous liver tissues from patients with primary hepatocellular carcinoma (HCC).METHODS: Using a reverse-transcription polymerase chain reaction (RT-PCR)-based assay, VEGF mRNA was determined prospectively in noncancerous liver tissues from 60 consecutive patients with HCC undergoing curative resection. We categorized the patients with VEGF165 mRNA over 0.500 in noncancerous liver tissues as group A, and those below 0.500 as group B.RESULTS: Among the isoforms of VEGF mRNA by multivariate analysis, a higher level of VEGF165 mRNA in noncancerous liver tissue correlated significantly with a higher risk of HCC recurrence (P = 0.039) and recurrence-related mortality (P= 0.048), but VEGF121 did not. The other significant predictors of recurrence consisted of vascular permeation (P = 0.022),daughter nodules (P = 0.033), cellular dedifferentiation (P = 0.033), an absent or incomplete capsule (P = 0.037).A significant variable of recurrence-related mortality was Vascular permeation (P= 0.012). As to the clinical manifestations of 16 patients who developed recurrence,the recurrent tumor number over 2, recurrent extent over two-liver segments, and the median survival after recurrence,all significantly correlated with group A patients (P = 0.043,0.043, and 0.048, respectively). However, the presence of extrahepatic metastasis was not (P＞0.05). The difference in recurrence after treatment between the two groups had no statistical significance (P＞0.05).CONCLUSION: The higher expression of isoform VEGF165mRNA in noncancerous liver remnant of patients with HCC may be a significant biological indicator of the invasiveness of postoperative recurrence.展开更多
To explore the relationship between senescent pulmonary microvascular endothelial cells (PMVECs) in-duced by D-galactose (D-gal) and apoptosis, PMVECs were cultured with DMEM containing D-gal (10 g/L) and identi-fied ...To explore the relationship between senescent pulmonary microvascular endothelial cells (PMVECs) in-duced by D-galactose (D-gal) and apoptosis, PMVECs were cultured with DMEM containing D-gal (10 g/L) and identi-fied by the following methods. We found that about 90% senescent cells were b-galactosidase positive cells, and 90% cells entered an irreversible G1 growth arrest. The cells in S and G2/M phases nearly disappeared. These results indi-cated that PMVECs ageing model induced by D-gal was es-tablished successfully. Compared with the control, PMVECs induced by D-gal showed that chromatin condensation and apoptotic bodies in nucleus were detected by fluorescence microscopy and transmission electron microscopy. Apoptotic cells in the early and middle phases increased significantly (39.8 2.8)% vs. (14.0 3.7)%. Typical Sub-G1 peak in the late apoptosis phase appeared in the terminal stage of PMVECs ageing. It is concluded that D-gal induces cultured PMVECs replicative senescence. Senescent PMVECs in-duced by D-gal were sensitive to apoptosis. Apoptosis is the regular feature of senescent PMVECs.展开更多
基金Supported by the Grants from the Department of Medical Research,Mackay Memorial Hospital, Taiwan, China (MMH9237)
文摘AIM: To investigate the prognostic role of isoform 165 vascular endothelial growth factor messenger RNA (VEGF165 mRNA)in noncancerous liver tissues from patients with primary hepatocellular carcinoma (HCC).METHODS: Using a reverse-transcription polymerase chain reaction (RT-PCR)-based assay, VEGF mRNA was determined prospectively in noncancerous liver tissues from 60 consecutive patients with HCC undergoing curative resection. We categorized the patients with VEGF165 mRNA over 0.500 in noncancerous liver tissues as group A, and those below 0.500 as group B.RESULTS: Among the isoforms of VEGF mRNA by multivariate analysis, a higher level of VEGF165 mRNA in noncancerous liver tissue correlated significantly with a higher risk of HCC recurrence (P = 0.039) and recurrence-related mortality (P= 0.048), but VEGF121 did not. The other significant predictors of recurrence consisted of vascular permeation (P = 0.022),daughter nodules (P = 0.033), cellular dedifferentiation (P = 0.033), an absent or incomplete capsule (P = 0.037).A significant variable of recurrence-related mortality was Vascular permeation (P= 0.012). As to the clinical manifestations of 16 patients who developed recurrence,the recurrent tumor number over 2, recurrent extent over two-liver segments, and the median survival after recurrence,all significantly correlated with group A patients (P = 0.043,0.043, and 0.048, respectively). However, the presence of extrahepatic metastasis was not (P＞0.05). The difference in recurrence after treatment between the two groups had no statistical significance (P＞0.05).CONCLUSION: The higher expression of isoform VEGF165mRNA in noncancerous liver remnant of patients with HCC may be a significant biological indicator of the invasiveness of postoperative recurrence.
基金supported by the National Major Basic Research Program in China(Grant No.G2000057004)the National Natural Science Foundation of China(Grant No.30170400).
文摘To explore the relationship between senescent pulmonary microvascular endothelial cells (PMVECs) in-duced by D-galactose (D-gal) and apoptosis, PMVECs were cultured with DMEM containing D-gal (10 g/L) and identi-fied by the following methods. We found that about 90% senescent cells were b-galactosidase positive cells, and 90% cells entered an irreversible G1 growth arrest. The cells in S and G2/M phases nearly disappeared. These results indi-cated that PMVECs ageing model induced by D-gal was es-tablished successfully. Compared with the control, PMVECs induced by D-gal showed that chromatin condensation and apoptotic bodies in nucleus were detected by fluorescence microscopy and transmission electron microscopy. Apoptotic cells in the early and middle phases increased significantly (39.8 2.8)% vs. (14.0 3.7)%. Typical Sub-G1 peak in the late apoptosis phase appeared in the terminal stage of PMVECs ageing. It is concluded that D-gal induces cultured PMVECs replicative senescence. Senescent PMVECs in-duced by D-gal were sensitive to apoptosis. Apoptosis is the regular feature of senescent PMVECs.
