AIM: To investigate the effectiveness of 4 d' anti-Helicobacter pylori therapy on the H pylori-infected Mongolian gerbils based on physiological and pathological changes. METHODS: We used 6-wk-old male gerbils ora...AIM: To investigate the effectiveness of 4 d' anti-Helicobacter pylori therapy on the H pylori-infected Mongolian gerbils based on physiological and pathological changes. METHODS: We used 6-wk-old male gerbils orally inoculated with H pylori (ATCC43504, 2×108 CFU/mL). Seven weeks after H pylori inoculation, the animals of study group received 4 d' anti-H pylori triple therapy (H pylori-eradicated group). Seven days later, all animals of the H pylori-eradicated and control groups (H pylori-infected & H pylori-uninfected groups) were sacrificed. We examined gastric mucosal lesions macroscopically, studied gastritis microscopically and determined the stomach weight ratio, myeloperoxidase (MPO) activity and prostaglandin (PG) E2 level. RESULTS: The results showed that both macroscopic and histological gastric damages were significantly less in H pylori-eradicated group than H pylori-infected group. Stomach weight ratio, MPO activity and PGE2 levels were significantly higher in H pylori-infected group than those in the other two groups. CONCLUSION: Four days' anti-H pylori therapy was effective in the improvement of H pylori-induced gastric lesions in Mongolian gerbils.展开更多
目的:使用动物模型,探讨消化性溃疡两个危险因素-非甾体类药物和幽门螺杆菌感染 Hp 之间的相互作用.方法:使用 SPF 级砂土鼠,非甾体类药物选用消炎痛针剂,幽门螺杆菌选用国际标准菌株,培养后接种于砂土鼠胃内.实验共分为四组:1组:单纯...目的:使用动物模型,探讨消化性溃疡两个危险因素-非甾体类药物和幽门螺杆菌感染 Hp 之间的相互作用.方法:使用 SPF 级砂土鼠,非甾体类药物选用消炎痛针剂,幽门螺杆菌选用国际标准菌株,培养后接种于砂土鼠胃内.实验共分为四组:1组:单纯胃内接种 Hp 菌液;2组:单纯皮下注射消炎痛;3组:皮下注射消炎痛后接种 Hp 菌液;4组:对照组,单纯灌胃培养液.2wk 后每组各处死2只鼠,做组织学检查,20只鼠做除菌治疗,余下鼠继续观察,3mo后处死,观察胃黏膜改变.结果:胃内接种 Hp 菌液组,2wk 后少量炎性细胞浸润,3mo 后炎症反应轻微;消炎痛组2wk 后胃黏膜表面充血、水肿,3mo 后黏膜炎症消失;消炎痛后接种 Hp 菌液组:2wk后炎症反应明显,3mo 后看到溃疡形成及大量 Hp 定植,对照组无异常改变.除菌治疗后,炎症明显好转.结论:消炎痛的应用使 Hp 接种后胃黏膜的损害明显加重,增加了胃黏膜对 Hp 的敏感性,使胃溃疡发生的危险性增加,应用消炎痛和 Hp 感染之间呈协同作用,根除 Hp 可有效防止胃溃疡的发生.展开更多
文摘AIM: To investigate the effectiveness of 4 d' anti-Helicobacter pylori therapy on the H pylori-infected Mongolian gerbils based on physiological and pathological changes. METHODS: We used 6-wk-old male gerbils orally inoculated with H pylori (ATCC43504, 2×108 CFU/mL). Seven weeks after H pylori inoculation, the animals of study group received 4 d' anti-H pylori triple therapy (H pylori-eradicated group). Seven days later, all animals of the H pylori-eradicated and control groups (H pylori-infected & H pylori-uninfected groups) were sacrificed. We examined gastric mucosal lesions macroscopically, studied gastritis microscopically and determined the stomach weight ratio, myeloperoxidase (MPO) activity and prostaglandin (PG) E2 level. RESULTS: The results showed that both macroscopic and histological gastric damages were significantly less in H pylori-eradicated group than H pylori-infected group. Stomach weight ratio, MPO activity and PGE2 levels were significantly higher in H pylori-infected group than those in the other two groups. CONCLUSION: Four days' anti-H pylori therapy was effective in the improvement of H pylori-induced gastric lesions in Mongolian gerbils.
文摘目的:使用动物模型,探讨消化性溃疡两个危险因素-非甾体类药物和幽门螺杆菌感染 Hp 之间的相互作用.方法:使用 SPF 级砂土鼠,非甾体类药物选用消炎痛针剂,幽门螺杆菌选用国际标准菌株,培养后接种于砂土鼠胃内.实验共分为四组:1组:单纯胃内接种 Hp 菌液;2组:单纯皮下注射消炎痛;3组:皮下注射消炎痛后接种 Hp 菌液;4组:对照组,单纯灌胃培养液.2wk 后每组各处死2只鼠,做组织学检查,20只鼠做除菌治疗,余下鼠继续观察,3mo后处死,观察胃黏膜改变.结果:胃内接种 Hp 菌液组,2wk 后少量炎性细胞浸润,3mo 后炎症反应轻微;消炎痛组2wk 后胃黏膜表面充血、水肿,3mo 后黏膜炎症消失;消炎痛后接种 Hp 菌液组:2wk后炎症反应明显,3mo 后看到溃疡形成及大量 Hp 定植,对照组无异常改变.除菌治疗后,炎症明显好转.结论:消炎痛的应用使 Hp 接种后胃黏膜的损害明显加重,增加了胃黏膜对 Hp 的敏感性,使胃溃疡发生的危险性增加,应用消炎痛和 Hp 感染之间呈协同作用,根除 Hp 可有效防止胃溃疡的发生.
