目的翻译纪念斯隆-凯特林癌症中心（Memorial Sloan-Kettering Cancer Center，MSKCC）肠道功能问卷并验证其在我国直肠癌保肛术后患者肠道功能评价中的信效度。方法经过翻译、回译和文化调试确定中文版问卷。采用寄信或门诊调查的方式...目的翻译纪念斯隆-凯特林癌症中心（Memorial Sloan-Kettering Cancer Center，MSKCC）肠道功能问卷并验证其在我国直肠癌保肛术后患者肠道功能评价中的信效度。方法经过翻译、回译和文化调试确定中文版问卷。采用寄信或门诊调查的方式对102例患者进行问卷调查。效度通过探索性因子分析、校标关联效度和已知组别效度检验：信度通过Cronbach’s α系数和重测信度检验。结果中文版MSKCC肠道功能问卷经因子分析提取出3个因子．累计方差贡献率为50．00％。总分及3个因子与生活质量的多项维度均具有明显相关性（均P〈0．05）。除了“排便受饮食影响”因子外，其他2个因子得分和总分在不同临床特征的患者中的差异都有统计学意义。总分及各因子的Cronbach’s α系数为0．602—0．856，重测信度为0．662—0．893。结论中文版MSKCC肠道功能问卷具有良好的信效度，适用于评价直肠癌保肛术后患者的肠道功能。展开更多
试验选用日龄与健康状况相同的黄羽肉鸡120羽,分为3个试验组和1个对照组,试验组A、B、C日粮分别添加1‰、1.5‰、2‰的凝结芽孢杆菌制剂,65日龄时进行屠宰,测定生长性能、屠宰性能、肠道内菌群及小肠绒毛组织结构、肌肉主要营养成分含...试验选用日龄与健康状况相同的黄羽肉鸡120羽,分为3个试验组和1个对照组,试验组A、B、C日粮分别添加1‰、1.5‰、2‰的凝结芽孢杆菌制剂,65日龄时进行屠宰,测定生长性能、屠宰性能、肠道内菌群及小肠绒毛组织结构、肌肉主要营养成分含量。结果表明:凝结芽孢杆菌对促进黄羽肉鸡的生长作用显著,其中以1.5‰添加量效果最好;屠宰率和半净膛率无显著影响(P>0.05);对全净膛率有显著提高作用(P<0.05),其中以2‰添加量为最佳;试验组A、B、C肠道沙门氏菌分别为7.905±0.026 Lg cfu/g、6.433±0.092 Lg cfu/g和5.705±0.021 Lg cfu/g,显著低于对照组(P<0.05),试验A、B、C组大肠杆菌分别为7.517±0.022 Lg cfu/g、7.510±0.021 Lg cfu/g、7.4800±0.032 Lg cfu/g与对照组差异不显著(P>0.05),试验A、B、C组肠道内葡萄球菌分别为7.218±0.043 Lg cfu/g、6.885±0.031 Lg cfu/g、6.245±0.076 Lg cfu/g显著低于对照组(P<0.05);试验组绒毛高度显著高于对照组(P<0.05),隐窝深度显著低于对照组(P<0.05);绒毛高度与隐窝深度比值,3个试验组与对照组差异显著(P<0.05);对腿肌率、胸肌率都有显著提高作用(P<0.05),其中以2‰添加量最佳;凝结芽孢杆菌对肌肉粗灰分、嫩度、失水率均无显著影响(P>0.05);对蛋白含量影响不显著(P>0.05),凝结芽孢杆菌对胸肌和腿肌脂肪含量都有显著提高作用。总体来看,在日粮中添加1.5‰的凝结芽孢杆菌制剂,可以明显提高黄羽肉鸡的生产水平。展开更多
AIM: To investigate the protective effect of ketamine on the endotoxin-induced proinflammatory cytokines and NFkappa B activation in the intestine. ETHODS: Adult male Wistar rats were randomly divided into 6 groups: (...AIM: To investigate the protective effect of ketamine on the endotoxin-induced proinflammatory cytokines and NFkappa B activation in the intestine. ETHODS: Adult male Wistar rats were randomly divided into 6 groups: (a) normal saline control, (b) challenged with endotoxin (5 mg/kg) and treated by saline, (c) challenged with endotoxin (5 mg/kg) and treated by ketamine (0.5 mg/kg), (d) challenged with endotoxin (5 mg/kg) and treated by ketamine (5 mg/kg ), (e) challenged with endotoxin (5 mg/kg) and treated by ketamine (50 mg/kg), and (f) saline injected and treated by ketamine (50 mg/kg). After 1, 4 or 6 h, TNF-α and IL-6 mRNA were investigated in the tissues of the intestine (jejunum) by RT-PCR. TNF-α and IL-6 were measured by ELISA. We used electrophoretic mobility shift assay (EMSA) to investigate NF-kappa B activity in the intestine. RESULTS: NF-kappa B activity, the expression of TNF-α and IL-6 were enhanced in the intestine by endotoxin. Ketamine at a dose of 0.5 mg/kg could suppress endotoxininduced TNF-α mRNA and protein elevation and inhibit NFkappa B activation in the intestine. However the least dosage of ketamine to inhibit IL-6 was 5 mg/kg in our experiment. CONCLUSION: Ketamine can suppress endotoxin-induced production of proinflammatory cytokines such as TNF-α and IL-6 production in the intestine. This suppressive effect may act through inhibiting NF-kappa B.展开更多
文摘试验选用日龄与健康状况相同的黄羽肉鸡120羽,分为3个试验组和1个对照组,试验组A、B、C日粮分别添加1‰、1.5‰、2‰的凝结芽孢杆菌制剂,65日龄时进行屠宰,测定生长性能、屠宰性能、肠道内菌群及小肠绒毛组织结构、肌肉主要营养成分含量。结果表明:凝结芽孢杆菌对促进黄羽肉鸡的生长作用显著,其中以1.5‰添加量效果最好;屠宰率和半净膛率无显著影响(P>0.05);对全净膛率有显著提高作用(P<0.05),其中以2‰添加量为最佳;试验组A、B、C肠道沙门氏菌分别为7.905±0.026 Lg cfu/g、6.433±0.092 Lg cfu/g和5.705±0.021 Lg cfu/g,显著低于对照组(P<0.05),试验A、B、C组大肠杆菌分别为7.517±0.022 Lg cfu/g、7.510±0.021 Lg cfu/g、7.4800±0.032 Lg cfu/g与对照组差异不显著(P>0.05),试验A、B、C组肠道内葡萄球菌分别为7.218±0.043 Lg cfu/g、6.885±0.031 Lg cfu/g、6.245±0.076 Lg cfu/g显著低于对照组(P<0.05);试验组绒毛高度显著高于对照组(P<0.05),隐窝深度显著低于对照组(P<0.05);绒毛高度与隐窝深度比值,3个试验组与对照组差异显著(P<0.05);对腿肌率、胸肌率都有显著提高作用(P<0.05),其中以2‰添加量最佳;凝结芽孢杆菌对肌肉粗灰分、嫩度、失水率均无显著影响(P>0.05);对蛋白含量影响不显著(P>0.05),凝结芽孢杆菌对胸肌和腿肌脂肪含量都有显著提高作用。总体来看,在日粮中添加1.5‰的凝结芽孢杆菌制剂,可以明显提高黄羽肉鸡的生产水平。
文摘AIM: To investigate the protective effect of ketamine on the endotoxin-induced proinflammatory cytokines and NFkappa B activation in the intestine. ETHODS: Adult male Wistar rats were randomly divided into 6 groups: (a) normal saline control, (b) challenged with endotoxin (5 mg/kg) and treated by saline, (c) challenged with endotoxin (5 mg/kg) and treated by ketamine (0.5 mg/kg), (d) challenged with endotoxin (5 mg/kg) and treated by ketamine (5 mg/kg ), (e) challenged with endotoxin (5 mg/kg) and treated by ketamine (50 mg/kg), and (f) saline injected and treated by ketamine (50 mg/kg). After 1, 4 or 6 h, TNF-α and IL-6 mRNA were investigated in the tissues of the intestine (jejunum) by RT-PCR. TNF-α and IL-6 were measured by ELISA. We used electrophoretic mobility shift assay (EMSA) to investigate NF-kappa B activity in the intestine. RESULTS: NF-kappa B activity, the expression of TNF-α and IL-6 were enhanced in the intestine by endotoxin. Ketamine at a dose of 0.5 mg/kg could suppress endotoxininduced TNF-α mRNA and protein elevation and inhibit NFkappa B activation in the intestine. However the least dosage of ketamine to inhibit IL-6 was 5 mg/kg in our experiment. CONCLUSION: Ketamine can suppress endotoxin-induced production of proinflammatory cytokines such as TNF-α and IL-6 production in the intestine. This suppressive effect may act through inhibiting NF-kappa B.
