AIM: To investigate the influence of hepatic arterial blockage on blood perfusion of transplanted cancer in rat liver and the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-1 (MMP...AIM: To investigate the influence of hepatic arterial blockage on blood perfusion of transplanted cancer in rat liver and the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-1 (MMP-1), and to explore the mechanisms involved in transarterial embolization (TAE)-induced metastasis of liver cancer preliminarily. METHODS: Walker 256 carcinosarcoma was transplanted into rat liver to establish the liver cancer model. Hepatic arterial ligation (HAL) was used to block the hepatic arterial blood supply and simulate TAE. Blood perfusion of tumor in control, laparotomy control, and HAL group was analyzed by Hoechst 33342 labeling assay, the serum VEGF level was assayed by ELISA, the expression of VEGF and MMP-1 mRNA was detected by in situ hybridization. RESULTS: Two days after HAL, the number of Hoechst 33342 labeled cells which represent the blood perfusion of tumor directly and hypoxia of tumor indirectly in HAL group decreased significantly compared with that in control group (329+/-29 vs 384+/-19, P【0.01). The serum VEGF level in the HAL group increased significantly as against that of the control group (93 ng.L(-1)+/-44 ng.L(-1) vs 55 ng.L(-1)+/-19 ng.L(-1), P【0.05). The expression of VEGF and MMP-1 mRNA in the tumor tissue of the HAL group increased significantly compared with that of the control and the laparotomy control groups (P【0.05). The blood perfusion data of the tumor, represented by the number of Hoechst 33342 labeled cells, showed a good linear inverse correlation with the serum VEGF level (r=-0.606, P【0.05) and the expression of VEGF mRNA in the tumor tissue ( r =-0.338, P【0.01). CONCLUSION: Blockage of hepatic arterial blood supply results in decreased blood perfusion and increased expression of metastasis-associated genes VEGF and MMP-1 of transplanted liver cancer in rats. Decreased blood perfusion and hypoxia may be the major cause of up-regulated expression of VEGF.展开更多
1986年在回顾肝动脉结扎术(HAL)治疗肝恶性肿瘤二十年发展史时,Bengmark指出,目前正进入一个从生物学水平上认识肝动脉阻断治疗肝恶性肿瘤机理的新时代,并预言反复暂时性肝动脉阻断(Repeated Temporary Blockade of the Hepatie Artey R...1986年在回顾肝动脉结扎术(HAL)治疗肝恶性肿瘤二十年发展史时,Bengmark指出,目前正进入一个从生物学水平上认识肝动脉阻断治疗肝恶性肿瘤机理的新时代,并预言反复暂时性肝动脉阻断(Repeated Temporary Blockade of the Hepatie Artey RTBHA)——HAL第五代衍生技术——将成为治疗不可切除性肝恶性肿瘤的一种合理和理想的方法.两年后,RTBHA已在临床运用中取得令人鼓舞的结果.本文将介绍RTBHA的方法、生物学基础、临床及实验结果和有待探讨的问题.材料及方法1.肝动脉阻断器RTBHA使用一种可在体外控制的肝动脉阻断器,反复短期地阻断肝动脉血流.展开更多
基金Science and Technology Development Fund of Shanghai Municipality,No.004119086
文摘AIM: To investigate the influence of hepatic arterial blockage on blood perfusion of transplanted cancer in rat liver and the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-1 (MMP-1), and to explore the mechanisms involved in transarterial embolization (TAE)-induced metastasis of liver cancer preliminarily. METHODS: Walker 256 carcinosarcoma was transplanted into rat liver to establish the liver cancer model. Hepatic arterial ligation (HAL) was used to block the hepatic arterial blood supply and simulate TAE. Blood perfusion of tumor in control, laparotomy control, and HAL group was analyzed by Hoechst 33342 labeling assay, the serum VEGF level was assayed by ELISA, the expression of VEGF and MMP-1 mRNA was detected by in situ hybridization. RESULTS: Two days after HAL, the number of Hoechst 33342 labeled cells which represent the blood perfusion of tumor directly and hypoxia of tumor indirectly in HAL group decreased significantly compared with that in control group (329+/-29 vs 384+/-19, P【0.01). The serum VEGF level in the HAL group increased significantly as against that of the control group (93 ng.L(-1)+/-44 ng.L(-1) vs 55 ng.L(-1)+/-19 ng.L(-1), P【0.05). The expression of VEGF and MMP-1 mRNA in the tumor tissue of the HAL group increased significantly compared with that of the control and the laparotomy control groups (P【0.05). The blood perfusion data of the tumor, represented by the number of Hoechst 33342 labeled cells, showed a good linear inverse correlation with the serum VEGF level (r=-0.606, P【0.05) and the expression of VEGF mRNA in the tumor tissue ( r =-0.338, P【0.01). CONCLUSION: Blockage of hepatic arterial blood supply results in decreased blood perfusion and increased expression of metastasis-associated genes VEGF and MMP-1 of transplanted liver cancer in rats. Decreased blood perfusion and hypoxia may be the major cause of up-regulated expression of VEGF.
文摘1986年在回顾肝动脉结扎术(HAL)治疗肝恶性肿瘤二十年发展史时,Bengmark指出,目前正进入一个从生物学水平上认识肝动脉阻断治疗肝恶性肿瘤机理的新时代,并预言反复暂时性肝动脉阻断(Repeated Temporary Blockade of the Hepatie Artey RTBHA)——HAL第五代衍生技术——将成为治疗不可切除性肝恶性肿瘤的一种合理和理想的方法.两年后,RTBHA已在临床运用中取得令人鼓舞的结果.本文将介绍RTBHA的方法、生物学基础、临床及实验结果和有待探讨的问题.材料及方法1.肝动脉阻断器RTBHA使用一种可在体外控制的肝动脉阻断器,反复短期地阻断肝动脉血流.
