AIM: To assess vitamin D (Vit D) abnormalities in hepatitis C infected patients and their relationship with interleukin (IL)-17, IL-23 and N-terminal propeptide of type Ⅲ pro-collagen (PⅢNP) as immune response media...AIM: To assess vitamin D (Vit D) abnormalities in hepatitis C infected patients and their relationship with interleukin (IL)-17, IL-23 and N-terminal propeptide of type Ⅲ pro-collagen (PⅢNP) as immune response mediators. METHODS: The study was conducted on 50 Egyptian patients (36 male, 14 female) with hepatitis C virus (HCV) infection, who visited the Hepatology Outpatient Clinic in the Endemic Disease Hospital at Cairo University. Patients were compared with 25 ageand sexmatched healthy individuals. Inclusion criteria were based on a history of liver disease with HCV genotype 4 (HCV-4) infection (as new patients or under followup). Based on ultrasonography, patients were classified into four subgroups; 14 with bright hepatomegaly; 11 with perihepatic fibrosis; 11 with hepatic cirrhosis; and 14 with cirrhosis and hepatocellular carcinoma (HCC).Total Vit D (i.e., 25-OH-Vit D) and active Vit D [i.e., 1,25-(OH) 2 -Vit D] assays were carried out using commercial kits. IL-17, IL-23 and PⅢNP levels were assayed using enzyme linked immunosorbent assay kits, while HCV virus was measured by quantitative and qualitative polymerase chain reaction. RESULTS: Levels of Vit D and its active form were significantly lower in advanced liver disease (hepatic cirrhosis and/or carcinoma) patients, compared to those with bright hepatomegaly and perihepatic fibrosis. IL-17, IL-23 and PⅢNP levels were markedly increased in HCV patients and correlated with the progression of hepatic damage. The decrease in Vit D and active Vit D was concomitant with an increase in viral load, as well as levels of IL-17, IL-23 and PⅢNP among all subgroups of HCV-infected patients, compared to normal healthy controls. A significant negative correlation was evident between active Vit D and each of IL-17, IL-23 and PⅢNP (r = -0.679, -0.801 and -0.920 at P < 0.001, respectively). HCV-infected men and women showed no differences with respect to Vit D levels. The viral load was negatively correlated with Vit D and active Vit D (r = -0.084 and -0.84展开更多
目的探讨缺铁性贫血(iron deficiency anemia,IDA)青少年女性维生素D缺乏症(Vitamin D deficiency,VDD)发生率及其与铁代谢指标的相关性。方法选择2019年1月至2019年12月来连云港市第一人民医院门诊就诊的80例青少年女性作为研究对象。...目的探讨缺铁性贫血(iron deficiency anemia,IDA)青少年女性维生素D缺乏症(Vitamin D deficiency,VDD)发生率及其与铁代谢指标的相关性。方法选择2019年1月至2019年12月来连云港市第一人民医院门诊就诊的80例青少年女性作为研究对象。其中,IDA确诊50例(IDA组,n=50),另外30例为健康女性(对照组,n=30)。分析IDA青少年女性中VDD的发生率。比较IDA组和对照组患者血清铁、血清铁蛋白、总铁结合力(total iron binding capacity,TIBC)等铁代谢指标以及维生素D水平差异;比较IDA患者中不同维生素D水平下铁代谢指标水平差异;采用Pearson’s相关性分析IDA患者中维生素D水平与铁代谢指标的相关性。结果IDA组和对照组患者在体质量指数(Body mass index,BMI)、血红蛋白、平均红细胞体积(mean corpuscular volume,MCV)、红细胞比容、血清铁、血清铁蛋白、TIBC、血清维生素D方面差异有统计学意义(t=2.096,P=0.039;t=11.856,P<0.001;t=7.591,P<0.001;t=4.194,P<0.001;t=12.492,P<0.001;t=12.572,P<0.001;t=3.152,P=0.002;t=3.626,P=0.001)。IDA组VDD患者21例(42%),对照组VDD患者5例(16.67%),两组比较差异有统计学意义(χ^(2)=5.485,P=0.019)。Pearson’s相关性分析结果显示,IDA患者中维生素D水平与血清铁、血清铁蛋白、TIBC无相关性(r=-0.081,P=0.658;r=0.029,P=0.883;r=0.141,P=0.394)。IDA患者中维生素D<30nmol/L组和维生素D>30nmol/L组在TIBC、血清维生素D方面差异有统计学意义(t=2.244,P=0.029;t=12.993,P<0.001)。结论VDD在患有IDA的青少年女性中的发生率高于健康对照组。然而,维生素D水平与铁代谢指标无显著相关性。展开更多
文摘AIM: To assess vitamin D (Vit D) abnormalities in hepatitis C infected patients and their relationship with interleukin (IL)-17, IL-23 and N-terminal propeptide of type Ⅲ pro-collagen (PⅢNP) as immune response mediators. METHODS: The study was conducted on 50 Egyptian patients (36 male, 14 female) with hepatitis C virus (HCV) infection, who visited the Hepatology Outpatient Clinic in the Endemic Disease Hospital at Cairo University. Patients were compared with 25 ageand sexmatched healthy individuals. Inclusion criteria were based on a history of liver disease with HCV genotype 4 (HCV-4) infection (as new patients or under followup). Based on ultrasonography, patients were classified into four subgroups; 14 with bright hepatomegaly; 11 with perihepatic fibrosis; 11 with hepatic cirrhosis; and 14 with cirrhosis and hepatocellular carcinoma (HCC).Total Vit D (i.e., 25-OH-Vit D) and active Vit D [i.e., 1,25-(OH) 2 -Vit D] assays were carried out using commercial kits. IL-17, IL-23 and PⅢNP levels were assayed using enzyme linked immunosorbent assay kits, while HCV virus was measured by quantitative and qualitative polymerase chain reaction. RESULTS: Levels of Vit D and its active form were significantly lower in advanced liver disease (hepatic cirrhosis and/or carcinoma) patients, compared to those with bright hepatomegaly and perihepatic fibrosis. IL-17, IL-23 and PⅢNP levels were markedly increased in HCV patients and correlated with the progression of hepatic damage. The decrease in Vit D and active Vit D was concomitant with an increase in viral load, as well as levels of IL-17, IL-23 and PⅢNP among all subgroups of HCV-infected patients, compared to normal healthy controls. A significant negative correlation was evident between active Vit D and each of IL-17, IL-23 and PⅢNP (r = -0.679, -0.801 and -0.920 at P < 0.001, respectively). HCV-infected men and women showed no differences with respect to Vit D levels. The viral load was negatively correlated with Vit D and active Vit D (r = -0.084 and -0.84
文摘目的探讨缺铁性贫血(iron deficiency anemia,IDA)青少年女性维生素D缺乏症(Vitamin D deficiency,VDD)发生率及其与铁代谢指标的相关性。方法选择2019年1月至2019年12月来连云港市第一人民医院门诊就诊的80例青少年女性作为研究对象。其中,IDA确诊50例(IDA组,n=50),另外30例为健康女性(对照组,n=30)。分析IDA青少年女性中VDD的发生率。比较IDA组和对照组患者血清铁、血清铁蛋白、总铁结合力(total iron binding capacity,TIBC)等铁代谢指标以及维生素D水平差异;比较IDA患者中不同维生素D水平下铁代谢指标水平差异;采用Pearson’s相关性分析IDA患者中维生素D水平与铁代谢指标的相关性。结果IDA组和对照组患者在体质量指数(Body mass index,BMI)、血红蛋白、平均红细胞体积(mean corpuscular volume,MCV)、红细胞比容、血清铁、血清铁蛋白、TIBC、血清维生素D方面差异有统计学意义(t=2.096,P=0.039;t=11.856,P<0.001;t=7.591,P<0.001;t=4.194,P<0.001;t=12.492,P<0.001;t=12.572,P<0.001;t=3.152,P=0.002;t=3.626,P=0.001)。IDA组VDD患者21例(42%),对照组VDD患者5例(16.67%),两组比较差异有统计学意义(χ^(2)=5.485,P=0.019)。Pearson’s相关性分析结果显示,IDA患者中维生素D水平与血清铁、血清铁蛋白、TIBC无相关性(r=-0.081,P=0.658;r=0.029,P=0.883;r=0.141,P=0.394)。IDA患者中维生素D<30nmol/L组和维生素D>30nmol/L组在TIBC、血清维生素D方面差异有统计学意义(t=2.244,P=0.029;t=12.993,P<0.001)。结论VDD在患有IDA的青少年女性中的发生率高于健康对照组。然而,维生素D水平与铁代谢指标无显著相关性。
