Steady-state absorption spectroscopy,circular dichroism,and resonance Raman spectroscopy have been used to investigate the thermal stability of LH2 complex isolated from purple photosynthetic bacterium Rps.palustris.T...Steady-state absorption spectroscopy,circular dichroism,and resonance Raman spectroscopy have been used to investigate the thermal stability of LH2 complex isolated from purple photosynthetic bacterium Rps.palustris.The results show that:1)upon increasing the temperature,a transition from B800 and B850 to free bacteriochlorophyll(B780)happens;2)a gradual decrease and disappearance of CD signal in visible region occur;3)a shift of the frequency,belonging to C==C and C—C stretching vibration,to higher wavenumber takes place.It is suggested that LH2 complex can be dissociated in the presence of B800,B850 and carotenoids simultaneously.Single-exponential fitting on the dynamic decay curves gives the apparent time constants of hundreds of minutes for various pigments.展开更多
基金Supported by the National Natural Science Foundation of China(Grant No.20373079)
文摘Steady-state absorption spectroscopy,circular dichroism,and resonance Raman spectroscopy have been used to investigate the thermal stability of LH2 complex isolated from purple photosynthetic bacterium Rps.palustris.The results show that:1)upon increasing the temperature,a transition from B800 and B850 to free bacteriochlorophyll(B780)happens;2)a gradual decrease and disappearance of CD signal in visible region occur;3)a shift of the frequency,belonging to C==C and C—C stretching vibration,to higher wavenumber takes place.It is suggested that LH2 complex can be dissociated in the presence of B800,B850 and carotenoids simultaneously.Single-exponential fitting on the dynamic decay curves gives the apparent time constants of hundreds of minutes for various pigments.
