AIM： To examine whether polymorphisms in SULTIA1 and GSTP1 genes contribute to colorectal cancer development and whether they are associated with clinicopathological variables are not well identified. METHODS： We ex...AIM： To examine whether polymorphisms in SULTIA1 and GSTP1 genes contribute to colorectal cancer development and whether they are associated with clinicopathological variables are not well identified. METHODS： We examined the genotypes of 125 colorectal cancer patients and 666 healthy controls in a Swedish population by using PCR-restriction fragment length polymorphism （RFLP）. RESULTS： SULTIA1 ＊2/＊2 genotype （OR = 2.49, 95%CI = 1.48-4.19, P = 0.0002） and ＊2 allele （OR = 1.56, 95%CI =1.16-2.10, P = 0.002） had an effect on colorectal cancer susceptibility, while GSTP1 genotype was without effect. However, GSTP1 G-type predicted a worse prognosis in the patients independently of gender, age, Dukes＇ stage, growth pattern, and differentiation （P = 0.03）.CONCLUSION： Polymorphism in SULTIA1 may predispose to colorectal cancer and GSTP1 may be a biological indicator of prognosis in the patients.展开更多
Sulfotransferase （ST） is the first enzyme discovered in association with paralytic shellfish poisoning （PSP） toxin biosynthesis in toxic dinoflagellates. This study investigates the ST activity m crude enzyme extr...Sulfotransferase （ST） is the first enzyme discovered in association with paralytic shellfish poisoning （PSP） toxin biosynthesis in toxic dinoflagellates. This study investigates the ST activity m crude enzyme extraction of a toxic dinoflagellate species, Alexandrium tamarense CI01. The results show that crude enzyme can transfer a sulfate group from 3＇-phosphoadenosine 5＇-phosphosulfate （PAPS） to N-21 in the carbamoyl group of gonyautoxin 2/3 （GTX2/3） to produce C 1/C2, but is inactive toward STX to produce GTX5. The crude enzyme is optimally active at pH 6.0 and 15℃. The activity is enhanced by Co^2＋, Mg^2＋, Mn^2＋ and Ca^2＋ individually, but is inhibited by Cu^2＋. Moreover, the activity shows no difference when various sulfur compounds are used as sulfate donors. These results demonstrate that the ST specific to GTX2/3 is present in the cells of A. tamarense CI01 and is involved in PSP toxin biosynthesis. In addition, the ST from different dinoflagellates is species-specific, which explains well the various biosynthesis pathways of the PSP toxins in toxic dinoflagellates.展开更多
基金Supported by grants from the Swedish Cancer Foundation and the Health Research Council in the South-East of Sweden
文摘AIM： To examine whether polymorphisms in SULTIA1 and GSTP1 genes contribute to colorectal cancer development and whether they are associated with clinicopathological variables are not well identified. METHODS： We examined the genotypes of 125 colorectal cancer patients and 666 healthy controls in a Swedish population by using PCR-restriction fragment length polymorphism （RFLP）. RESULTS： SULTIA1 ＊2/＊2 genotype （OR = 2.49, 95%CI = 1.48-4.19, P = 0.0002） and ＊2 allele （OR = 1.56, 95%CI =1.16-2.10, P = 0.002） had an effect on colorectal cancer susceptibility, while GSTP1 genotype was without effect. However, GSTP1 G-type predicted a worse prognosis in the patients independently of gender, age, Dukes＇ stage, growth pattern, and differentiation （P = 0.03）.CONCLUSION： Polymorphism in SULTIA1 may predispose to colorectal cancer and GSTP1 may be a biological indicator of prognosis in the patients.
基金the National Natural Science Foundation of China (No.40376032)the Ministry of Science and Technology of the People’s Republic of China (No.2001CB409700)
文摘Sulfotransferase （ST） is the first enzyme discovered in association with paralytic shellfish poisoning （PSP） toxin biosynthesis in toxic dinoflagellates. This study investigates the ST activity m crude enzyme extraction of a toxic dinoflagellate species, Alexandrium tamarense CI01. The results show that crude enzyme can transfer a sulfate group from 3＇-phosphoadenosine 5＇-phosphosulfate （PAPS） to N-21 in the carbamoyl group of gonyautoxin 2/3 （GTX2/3） to produce C 1/C2, but is inactive toward STX to produce GTX5. The crude enzyme is optimally active at pH 6.0 and 15℃. The activity is enhanced by Co^2＋, Mg^2＋, Mn^2＋ and Ca^2＋ individually, but is inhibited by Cu^2＋. Moreover, the activity shows no difference when various sulfur compounds are used as sulfate donors. These results demonstrate that the ST specific to GTX2/3 is present in the cells of A. tamarense CI01 and is involved in PSP toxin biosynthesis. In addition, the ST from different dinoflagellates is species-specific, which explains well the various biosynthesis pathways of the PSP toxins in toxic dinoflagellates.
