AIM: To investigate whether the formation of aggregated HBx has a potential linking with its cellular responses.METHODS: Recombinant HBx was expressed in Escherichia coli and purified by Ni-NTA metal-affinity chromato...AIM: To investigate whether the formation of aggregated HBx has a potential linking with its cellular responses.METHODS: Recombinant HBx was expressed in Escherichia coli and purified by Ni-NTA metal-affinity chromatography.Anti-HBx monoclonal antibody was developed for immunocytochemical detection. Bicistronic expression vector harboring full-length DNA of HBx was employed for transfection of human HepG2 ceils. Immunocytochemical staining was used to examine the intracellular HBx aggregates in cells. The effects of HBx aggregation on cell cycle and apoptosis were assessed by flow cytometry.RESULTS: Immunocytochemical staining revealed most of the HBx was formed intracellular aggregate in cytoplasm and frequently accumulated in large granules. Flow cytometry analysis showed that HepG2 cells transfected with vector harboring HBx significantly increased apoptosis and largely accumulated in the G0-G1 phase by maintenance in serum medium for 36 hours. Control cells without HBx aggregates in the presence of serum entered S phase and proliferated more rapidly at the same time.EGFP fluorescence in HBx expression cells was significantly decreased.CONCLUSION: Our observations show that cells with HBx aggregate undergo growth arrest and apoptosis, whereas control cells without HBx remain in growth and progression into S phase. Our data may provide helpful information to understand the biological effects of HBx aggregates on cells.展开更多
近年来,在《古地理学报》(中文版和英文版)的已刊文章和来稿中,一些作者把岩石或岩石类型称作"微相"或"岩相",把岩石薄片中的微观特征称作"微相",把岩石的宏观特征称作"宏观相"。笔者曾写过2...近年来,在《古地理学报》(中文版和英文版)的已刊文章和来稿中,一些作者把岩石或岩石类型称作"微相"或"岩相",把岩石薄片中的微观特征称作"微相",把岩石的宏观特征称作"宏观相"。笔者曾写过2篇中文的短文《主编的话--岩石不是微相》和《主编的话--岩石不是岩相》,刊于《古地理学报》(中文版)中。但是此二文尚未引起国内外广大读者的关注。还有,在20世纪80年代,一些中国沉积学家根据野外露头和钻井岩心的岩石宏观特征,提出了"亚相"和"微相"。这个"微相"的定义与外国沉积学家在20世纪40年代根据岩石的显微镜下微观特征提出的"微相"的定义完全不同。这些问题屡屡出现从而迫使笔者,作为《古地理学报》(中文版和英文版)的主编,应该遵循"百花齐放和百家争鸣"的方针,再写出1篇中文及英文兼有的新文章,即《沉积相的一些术语定义的评论》(A review on the definitions of terms of sedimentary facies),把沉积相的一些术语,如"相"、"沉积相"、"岩相"、两种"微相"、"宏观相"、"亚相"等术语的定义讲清楚,并把此文同时在《古地理学报》(中文版和英文版)中刊出。希此文能引起国内外广大读者的关注,并撰写文章和参与这些问题讨论及争鸣,争取逐步得到一些共识,从而促进沉积学和古地理学的进步与发展。展开更多
文摘AIM: To investigate whether the formation of aggregated HBx has a potential linking with its cellular responses.METHODS: Recombinant HBx was expressed in Escherichia coli and purified by Ni-NTA metal-affinity chromatography.Anti-HBx monoclonal antibody was developed for immunocytochemical detection. Bicistronic expression vector harboring full-length DNA of HBx was employed for transfection of human HepG2 ceils. Immunocytochemical staining was used to examine the intracellular HBx aggregates in cells. The effects of HBx aggregation on cell cycle and apoptosis were assessed by flow cytometry.RESULTS: Immunocytochemical staining revealed most of the HBx was formed intracellular aggregate in cytoplasm and frequently accumulated in large granules. Flow cytometry analysis showed that HepG2 cells transfected with vector harboring HBx significantly increased apoptosis and largely accumulated in the G0-G1 phase by maintenance in serum medium for 36 hours. Control cells without HBx aggregates in the presence of serum entered S phase and proliferated more rapidly at the same time.EGFP fluorescence in HBx expression cells was significantly decreased.CONCLUSION: Our observations show that cells with HBx aggregate undergo growth arrest and apoptosis, whereas control cells without HBx remain in growth and progression into S phase. Our data may provide helpful information to understand the biological effects of HBx aggregates on cells.
文摘近年来,在《古地理学报》(中文版和英文版)的已刊文章和来稿中,一些作者把岩石或岩石类型称作"微相"或"岩相",把岩石薄片中的微观特征称作"微相",把岩石的宏观特征称作"宏观相"。笔者曾写过2篇中文的短文《主编的话--岩石不是微相》和《主编的话--岩石不是岩相》,刊于《古地理学报》(中文版)中。但是此二文尚未引起国内外广大读者的关注。还有,在20世纪80年代,一些中国沉积学家根据野外露头和钻井岩心的岩石宏观特征,提出了"亚相"和"微相"。这个"微相"的定义与外国沉积学家在20世纪40年代根据岩石的显微镜下微观特征提出的"微相"的定义完全不同。这些问题屡屡出现从而迫使笔者,作为《古地理学报》(中文版和英文版)的主编,应该遵循"百花齐放和百家争鸣"的方针,再写出1篇中文及英文兼有的新文章,即《沉积相的一些术语定义的评论》(A review on the definitions of terms of sedimentary facies),把沉积相的一些术语,如"相"、"沉积相"、"岩相"、两种"微相"、"宏观相"、"亚相"等术语的定义讲清楚,并把此文同时在《古地理学报》(中文版和英文版)中刊出。希此文能引起国内外广大读者的关注,并撰写文章和参与这些问题讨论及争鸣,争取逐步得到一些共识,从而促进沉积学和古地理学的进步与发展。
