The bacteria in the genus Vibrio are heterothrophic, which exist in the larval rearing water of Crustacea and often show diverse pathogenicities to marine animals. In order to assess the bacterial diversity associated...The bacteria in the genus Vibrio are heterothrophic, which exist in the larval rearing water of Crustacea and often show diverse pathogenicities to marine animals. In order to assess the bacterial diversity associated with Crustacean seed production, 32 strains were isolated from black tiger shrimp (Penaeus monodon) and mangrove crab (Scylla paramamosain) larvae and their rearing-water and characterized using biochemical and molecular approaches. Two or more genotypically different species were identified. The vibriosis of black tiger shrimp was caused by V. harveyi, V. alginolyticus and Vibrio spp. predominantly, while that of crab by V. harveyi and V. alginolyticus only.展开更多
The present paper reports 9 species of pontoniine shrimps collected from Hainan Island, South China Sea, by the Joint Chinese-German Marine Biology Expeditions, in which,Periclimenaeus arabicus(Calman, 1939) andPericl...The present paper reports 9 species of pontoniine shrimps collected from Hainan Island, South China Sea, by the Joint Chinese-German Marine Biology Expeditions, in which,Periclimenaeus arabicus(Calman, 1939) andPericlimenaeus hecate(Nobili, 1904) are recorded for the first time from Hainan Island.展开更多
Using a standard cytochrome c oxidase I sequence,DNA barcoding has been shown to be effective to distinguish known species and to discover cryptic species. Here we assessed the efficiency of DNA barcoding for the amph...Using a standard cytochrome c oxidase I sequence,DNA barcoding has been shown to be effective to distinguish known species and to discover cryptic species. Here we assessed the efficiency of DNA barcoding for the amphipod genus Gammarus from China. The maximum intraspecific divergence for widespread species,Gammarus lacustris,was 3.5%,and mean interspecific divergence reached 21.9%. We presented a conservative benchmark for determining provisional species using maximum intraspecific divergence of Gammarus lacustris. Thirty-one species possessed distinct barcode clusters. Two species were comprised of highly divergent clades with strong neighbor-joining bootstrap values,and likely indicated the presence of cryptic species. Although DNA barcoding is effective,future identification of species of Gammarus should incorporate DNA barcoding and morphological detection.展开更多
文摘The bacteria in the genus Vibrio are heterothrophic, which exist in the larval rearing water of Crustacea and often show diverse pathogenicities to marine animals. In order to assess the bacterial diversity associated with Crustacean seed production, 32 strains were isolated from black tiger shrimp (Penaeus monodon) and mangrove crab (Scylla paramamosain) larvae and their rearing-water and characterized using biochemical and molecular approaches. Two or more genotypically different species were identified. The vibriosis of black tiger shrimp was caused by V. harveyi, V. alginolyticus and Vibrio spp. predominantly, while that of crab by V. harveyi and V. alginolyticus only.
文摘The present paper reports 9 species of pontoniine shrimps collected from Hainan Island, South China Sea, by the Joint Chinese-German Marine Biology Expeditions, in which,Periclimenaeus arabicus(Calman, 1939) andPericlimenaeus hecate(Nobili, 1904) are recorded for the first time from Hainan Island.
基金supported by the National Natural Sciences Foundation of China (NSFC-30499341/30770268/30870473)Beijing Financial Budget Index (2008-NO.0178)the Beijing Natural Science Foundation (5082013)
文摘Using a standard cytochrome c oxidase I sequence,DNA barcoding has been shown to be effective to distinguish known species and to discover cryptic species. Here we assessed the efficiency of DNA barcoding for the amphipod genus Gammarus from China. The maximum intraspecific divergence for widespread species,Gammarus lacustris,was 3.5%,and mean interspecific divergence reached 21.9%. We presented a conservative benchmark for determining provisional species using maximum intraspecific divergence of Gammarus lacustris. Thirty-one species possessed distinct barcode clusters. Two species were comprised of highly divergent clades with strong neighbor-joining bootstrap values,and likely indicated the presence of cryptic species. Although DNA barcoding is effective,future identification of species of Gammarus should incorporate DNA barcoding and morphological detection.
