Objective:To explore the effect of “Runing Ⅱ” on the tumor growth and metastasis of Human Breast Cancer Cell Line MDA MB 435 in Vivo and Vitro. Methods: Both in vitro and in Vivo experiments were conducted in the s...Objective:To explore the effect of “Runing Ⅱ” on the tumor growth and metastasis of Human Breast Cancer Cell Line MDA MB 435 in Vivo and Vitro. Methods: Both in vitro and in Vivo experiments were conducted in the study. In Vitro, MTT method was used to detect the ability killing the tumor cells of serum with “Runing Ⅱ”. In vivo, We have observed the effect of “Runing Ⅱ” on the tumor growth and metastasis by nude mice model implanted MDA MB 435. Result: At the dosages of 32g, 16g and 8g per kilogram, “Runing Ⅱ” could kill MDA MB 435 cells 35 88%、29 84% and 8 30% respectively in vitro, the inhibitory effect of tumor growth was statistially significant, as compared with that of the normal group ( P <0 05),there were no statistital difference between the “Runing Ⅱ” group at the dosages of 48g/kg and the CTX group ( P>0 05 ). In the case of animal experiments, the Human Breast Cancer Cell Line MDA MB 435 was inoculated into the m.f.p. of nude mice, the results demonstrated that the growth suppression rate and the Lung metastasis inhibiting rate of “Runing Ⅱ” at the dosages of 48g/kg was 53 64% and 27% respectively, which were no statistial difference than that of CTX group (the growth suppression rate and the Lung metastasis inhibiting rate was 48 8% and 25 2% respectively)( P> 0 05). Conclusion: The results suggest that “Runing Ⅱ” can suppress the tumor growth and metastasis of Human Breast Cancer Cell Line MDA MB 435.展开更多
槐耳清膏的主要活性成分是多糖蛋白（polysaccharide of trametes robiniophila murr，PS—T），能诱导人直肠癌细胞株（HR8348细胞）发生凋亡，对血管内皮细胞体外构建新生血管有抑制作用。但槐耳清膏对肝癌在体内的生长和转移的干预...槐耳清膏的主要活性成分是多糖蛋白（polysaccharide of trametes robiniophila murr，PS—T），能诱导人直肠癌细胞株（HR8348细胞）发生凋亡，对血管内皮细胞体外构建新生血管有抑制作用。但槐耳清膏对肝癌在体内的生长和转移的干预作用尚无报道。本研究利用裸鼠人肝癌转移模型LCI-D20进行了抑制肿瘤生长和转移的动物实验，现报道如下。展开更多
文摘Objective:To explore the effect of “Runing Ⅱ” on the tumor growth and metastasis of Human Breast Cancer Cell Line MDA MB 435 in Vivo and Vitro. Methods: Both in vitro and in Vivo experiments were conducted in the study. In Vitro, MTT method was used to detect the ability killing the tumor cells of serum with “Runing Ⅱ”. In vivo, We have observed the effect of “Runing Ⅱ” on the tumor growth and metastasis by nude mice model implanted MDA MB 435. Result: At the dosages of 32g, 16g and 8g per kilogram, “Runing Ⅱ” could kill MDA MB 435 cells 35 88%、29 84% and 8 30% respectively in vitro, the inhibitory effect of tumor growth was statistially significant, as compared with that of the normal group ( P <0 05),there were no statistital difference between the “Runing Ⅱ” group at the dosages of 48g/kg and the CTX group ( P>0 05 ). In the case of animal experiments, the Human Breast Cancer Cell Line MDA MB 435 was inoculated into the m.f.p. of nude mice, the results demonstrated that the growth suppression rate and the Lung metastasis inhibiting rate of “Runing Ⅱ” at the dosages of 48g/kg was 53 64% and 27% respectively, which were no statistial difference than that of CTX group (the growth suppression rate and the Lung metastasis inhibiting rate was 48 8% and 25 2% respectively)( P> 0 05). Conclusion: The results suggest that “Runing Ⅱ” can suppress the tumor growth and metastasis of Human Breast Cancer Cell Line MDA MB 435.
文摘槐耳清膏的主要活性成分是多糖蛋白（polysaccharide of trametes robiniophila murr，PS—T），能诱导人直肠癌细胞株（HR8348细胞）发生凋亡，对血管内皮细胞体外构建新生血管有抑制作用。但槐耳清膏对肝癌在体内的生长和转移的干预作用尚无报道。本研究利用裸鼠人肝癌转移模型LCI-D20进行了抑制肿瘤生长和转移的动物实验，现报道如下。
