Following a brief account of the early foundations of seed germination ecology sensu lato, some historical and recent developments pertaining to the ecology, biogeography and phylogeny of seed dormancy are discussed.
目的 探讨人乳头状瘤病毒(hpv)16的存在状态及晚期蛋白l1表达与宫颈病变程度的关系.方法 收集61例宫颈病变患者为研究对象,按病理学诊断结果分为慢性宫颈炎组(27例),cin Ⅰ、cinⅡ组(10例),原位癌组(8例),早期浸润癌组(7例)和中晚期癌...目的 探讨人乳头状瘤病毒(hpv)16的存在状态及晚期蛋白l1表达与宫颈病变程度的关系.方法 收集61例宫颈病变患者为研究对象,按病理学诊断结果分为慢性宫颈炎组(27例),cin Ⅰ、cinⅡ组(10例),原位癌组(8例),早期浸润癌组(7例)和中晚期癌组(9例).采用pcr方法检测hpv分型及生物状态,采用免疫印迹法(western blot)测定晚期蛋白l1表达.组间比较采用kruskal-wallis检验,hpv16生物状态、l1蛋白表达水平与病变程度的关系采用pearson相关分析.结果 61例宫颈病变患者检出hpv16型53例,占86.9%,其中25例慢性宫颈炎患者均为游离型;cin Ⅰ、cinⅡ组6例游离型,2例整合型;原位癌组1例游离型,3例混合型和3例整合型;早期浸润癌组2例混合型,4例整合型;中晚期癌组1例混合型,6例整合型.hpv16整合型与宫颈病变程度呈正相关(r=0.705,p＜0.01).随着宫颈病变损伤程度加重,l1蛋白表达逐渐减弱,与宫颈病变程度呈负相关(r=-0.755,p＜0.01).结论 hpv16整合型及l1蛋白表达下降预示宫颈病变程度加重.
abstract：
objective to evaluate the relationship of biological status of hpv 16 and expression of l1 protein with the degree of cervical tumorigenesis.methods sixty-one patients with cervical lessions were enrolled and divided into five groups according to pathology of cervical lesions,including chronic cervicitis(n = 27),cervical intraepithelial neoplasia(cin) Ⅰ-Ⅱ(n = 10),carcinoma in situ(n = 8),early-stage(n =7)and mid/late-stage(n = 9)of cervical carcinoma.hpv type and its biological status were detected by pcr amplification,and l1 protein in hpv 16 positive tissues was detected by western blot.kruskal-wallis was used to compare between the groups,and pearson correlation analysis was used to evaluate the relationship of hpv biological status and l1 protain expression with cervical lesions.results fifty-three out of 61 patients with cervical lesions were detected with hpv positive(86.9%).all 25 patients with chronic展开更多
A novel method was developed to use glutinous broomcorn millets (Panicum miliaceum L.) as solid substrate to make cultures of the entomophthoralean fungus Pandora delphacis specifically pathogenic to planthoppers, lea...A novel method was developed to use glutinous broomcorn millets (Panicum miliaceum L.) as solid substrate to make cultures of the entomophthoralean fungus Pandora delphacis specifically pathogenic to planthoppers, leafhop-pers and aphids. Steamed millets with water content of 45% were inoculated with a liquid culture of P. delphacis at a ratio of 20% (v/w) and then incubated at 25℃ and L:D 12:12. The millets cultured for 3—17 d exhibited high potential for co-nidial production. The 5-d-old millet culture sporulated most abundantly, discharging up to 17.12 (1.31) 104 conidia/ millet. The cultures incubated for 7—11 d also had a satis-factory sporulation capability, yielding 13.00—13.90 104 conidia/millet. Compared to 2.32 (0.34) 104 conidia dis-charged from each of Myzus persicae adults killed by P. del-phacis and a ≤60-h duration of sporulation, each of the millets cultured for 5—11 d produced 5.6—7.4 times more conidia with an over doubled duration for conidial discharge (144 h). Among 106 M. persicae adults exposed to the shower of conidia discharged from the cultured millets, a total mor-tality of 69.8% caused by P. delphacis infection was observed within 7 d after exposure, but no death was attributed to the fungal infection in the aphids unexposed. The results indi-cate that the millet cultures of P. delphacis are biologically similar to aphid cadavers killed by the same fungus. Due to the superiority of the cultured millets to the cadavers in sporulation potential and duration, the method for making cultures of P. delphacis on the broomcorn millets is highly recommended for use in study of entomophthoralean fungi for microbial control. This is the first report on the success of the solid culture of Pandora species on cereals.展开更多
Ghrelin was isolated as an endogenous ligand for the GH secretagogue receptor from the rat stomach. Although physiological effects of ghrelin have been revealed by numerous studies, the regulation of stomach ghrelin r...Ghrelin was isolated as an endogenous ligand for the GH secretagogue receptor from the rat stomach. Although physiological effects of ghrelin have been revealed by numerous studies, the regulation of stomach ghrelin remains obscure, and the factor that directly regulates ghrelin expression and production has not been identified. Here, we show some data regarding the characteristic features of ghrelin cells and the regulation of stomach ghrelin. In the gastrointestinal tract, ghrelin cells were identified as opened- and closed-type cells, and it was found that the number of ghrelin cells decreased from the stomach to the colon. The postnatal change in number of ghrelin cells in the stomach showed a sexually dimorphic pattern, indicating a role of estrogen in the regulation of stomach ghrelin. In vitro studies revealed that estrogen stimulated both ghrelin expression and production and that treatment with formestane, an aromatase (estrogen synthetase) inhibitor, decreased ghrelin expression level. On the other hand, leptin was found to inhibit both basal and estrogen-stimulated ghrelin expression. Moreover, both aromatase mRNA- expressing cells and leptin cells were found to be located close to ghrelin cells in the gastric mucosa. Furthermore, we found an inverse relationship between gastric ghrelin and leptin levels in a fasting state, and we revealed relative changes in expression of gastric ghrelin, estrogen and leptin in the postnatal rats. We propose that gastric estrogen and leptin directly regulate stomach ghrelin and that the balance control through gastric estrogen and leptin contributes to the altered ghrelin expression level in some physiological states.展开更多
The application of BEM for solving normal or inverse bio-heat transfer problems of biological bodies with complex shapes is discussed and a new method for non-invasive reconstruction of the spatial temperature field a...The application of BEM for solving normal or inverse bio-heat transfer problems of biological bodies with complex shapes is discussed and a new method for non-invasive reconstruction of the spatial temperature field and the non-homogeneous heat source is proposed in this paper. Tikhonov's (1979) regularization technique is used to improve the performance of ill-posed equations in the process of solving for inverse problems. The feasibility of the above mentioned method was verified by the numerical test results of a two-dimensional steady state problem. This method has laid the theoretical foundation for developing a biomedical instrument and respective software used in the non-invasive monitoring of the spatial temperature field of biological bodies.展开更多
文摘Following a brief account of the early foundations of seed germination ecology sensu lato, some historical and recent developments pertaining to the ecology, biogeography and phylogeny of seed dormancy are discussed.
文摘目的 探讨人乳头状瘤病毒(hpv)16的存在状态及晚期蛋白l1表达与宫颈病变程度的关系.方法 收集61例宫颈病变患者为研究对象,按病理学诊断结果分为慢性宫颈炎组(27例),cin Ⅰ、cinⅡ组(10例),原位癌组(8例),早期浸润癌组(7例)和中晚期癌组(9例).采用pcr方法检测hpv分型及生物状态,采用免疫印迹法(western blot)测定晚期蛋白l1表达.组间比较采用kruskal-wallis检验,hpv16生物状态、l1蛋白表达水平与病变程度的关系采用pearson相关分析.结果 61例宫颈病变患者检出hpv16型53例,占86.9%,其中25例慢性宫颈炎患者均为游离型;cin Ⅰ、cinⅡ组6例游离型,2例整合型;原位癌组1例游离型,3例混合型和3例整合型;早期浸润癌组2例混合型,4例整合型;中晚期癌组1例混合型,6例整合型.hpv16整合型与宫颈病变程度呈正相关(r=0.705,p＜0.01).随着宫颈病变损伤程度加重,l1蛋白表达逐渐减弱,与宫颈病变程度呈负相关(r=-0.755,p＜0.01).结论 hpv16整合型及l1蛋白表达下降预示宫颈病变程度加重.
abstract：
objective to evaluate the relationship of biological status of hpv 16 and expression of l1 protein with the degree of cervical tumorigenesis.methods sixty-one patients with cervical lessions were enrolled and divided into five groups according to pathology of cervical lesions,including chronic cervicitis(n = 27),cervical intraepithelial neoplasia(cin) Ⅰ-Ⅱ(n = 10),carcinoma in situ(n = 8),early-stage(n =7)and mid/late-stage(n = 9)of cervical carcinoma.hpv type and its biological status were detected by pcr amplification,and l1 protein in hpv 16 positive tissues was detected by western blot.kruskal-wallis was used to compare between the groups,and pearson correlation analysis was used to evaluate the relationship of hpv biological status and l1 protain expression with cervical lesions.results fifty-three out of 61 patients with cervical lesions were detected with hpv positive(86.9%).all 25 patients with chronic
基金supported by the National Natural Science Foundation of China(Grant No.30070514)the Special Fund for Graduate Study Programs in Chinese Universities(Grant No.200203335041)the‘Cheung Kong Scholars Programme’,Ministry of Education,China.
文摘A novel method was developed to use glutinous broomcorn millets (Panicum miliaceum L.) as solid substrate to make cultures of the entomophthoralean fungus Pandora delphacis specifically pathogenic to planthoppers, leafhop-pers and aphids. Steamed millets with water content of 45% were inoculated with a liquid culture of P. delphacis at a ratio of 20% (v/w) and then incubated at 25℃ and L:D 12:12. The millets cultured for 3—17 d exhibited high potential for co-nidial production. The 5-d-old millet culture sporulated most abundantly, discharging up to 17.12 (1.31) 104 conidia/ millet. The cultures incubated for 7—11 d also had a satis-factory sporulation capability, yielding 13.00—13.90 104 conidia/millet. Compared to 2.32 (0.34) 104 conidia dis-charged from each of Myzus persicae adults killed by P. del-phacis and a ≤60-h duration of sporulation, each of the millets cultured for 5—11 d produced 5.6—7.4 times more conidia with an over doubled duration for conidial discharge (144 h). Among 106 M. persicae adults exposed to the shower of conidia discharged from the cultured millets, a total mor-tality of 69.8% caused by P. delphacis infection was observed within 7 d after exposure, but no death was attributed to the fungal infection in the aphids unexposed. The results indi-cate that the millet cultures of P. delphacis are biologically similar to aphid cadavers killed by the same fungus. Due to the superiority of the cultured millets to the cadavers in sporulation potential and duration, the method for making cultures of P. delphacis on the broomcorn millets is highly recommended for use in study of entomophthoralean fungi for microbial control. This is the first report on the success of the solid culture of Pandora species on cereals.
基金Supported by (in part) Grants for research fellowships from the Japan Society for the Promotion of Science for Young Scientists by the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation (NIBIO)
文摘Ghrelin was isolated as an endogenous ligand for the GH secretagogue receptor from the rat stomach. Although physiological effects of ghrelin have been revealed by numerous studies, the regulation of stomach ghrelin remains obscure, and the factor that directly regulates ghrelin expression and production has not been identified. Here, we show some data regarding the characteristic features of ghrelin cells and the regulation of stomach ghrelin. In the gastrointestinal tract, ghrelin cells were identified as opened- and closed-type cells, and it was found that the number of ghrelin cells decreased from the stomach to the colon. The postnatal change in number of ghrelin cells in the stomach showed a sexually dimorphic pattern, indicating a role of estrogen in the regulation of stomach ghrelin. In vitro studies revealed that estrogen stimulated both ghrelin expression and production and that treatment with formestane, an aromatase (estrogen synthetase) inhibitor, decreased ghrelin expression level. On the other hand, leptin was found to inhibit both basal and estrogen-stimulated ghrelin expression. Moreover, both aromatase mRNA- expressing cells and leptin cells were found to be located close to ghrelin cells in the gastric mucosa. Furthermore, we found an inverse relationship between gastric ghrelin and leptin levels in a fasting state, and we revealed relative changes in expression of gastric ghrelin, estrogen and leptin in the postnatal rats. We propose that gastric estrogen and leptin directly regulate stomach ghrelin and that the balance control through gastric estrogen and leptin contributes to the altered ghrelin expression level in some physiological states.
文摘The application of BEM for solving normal or inverse bio-heat transfer problems of biological bodies with complex shapes is discussed and a new method for non-invasive reconstruction of the spatial temperature field and the non-homogeneous heat source is proposed in this paper. Tikhonov's (1979) regularization technique is used to improve the performance of ill-posed equations in the process of solving for inverse problems. The feasibility of the above mentioned method was verified by the numerical test results of a two-dimensional steady state problem. This method has laid the theoretical foundation for developing a biomedical instrument and respective software used in the non-invasive monitoring of the spatial temperature field of biological bodies.
