Green fluorescent protein (GFP) and its variants /homolog proteins are generally called as GFP-like fluorescent proteins (FPs), which are widely used as visible molecular tools for monitoring a wide range of biologica...Green fluorescent protein (GFP) and its variants /homolog proteins are generally called as GFP-like fluorescent proteins (FPs), which are widely used as visible molecular tools for monitoring a wide range of biological processes due to their capability of simple, accurate and real time quantification. The FPs-based molecular and visible quantification tools are giving more impact on bioprocess engineering, enabling the biomolecule-level dynamic information to be linked with the process-level events. In this review, different applications of FPs in biological engineering with emphasis on rapid molecular bioprocess quantification, such as quantification of the transcription efficiency, the protein production, the protein folding efficiency, the cell concentration, the intracellular microenvironments and so on, would be first introduced. The challenges of using FPs with respect to actual bioprocess applications for the precise quantification including the interaction of FPs and the fused partner proteins, the maturation of FPs, the inner filter effect and sensing technology were then discussed. Finally, the future development for the FPs used in molecular bioprocess quantification would be proposed.展开更多
Fault monitoring of bioprocess is important to ensure safety of a reactor and maintain high quality of products. It is difficult to build an accurate mechanistic model for a bioprocess, so fault monitoring based on ri...Fault monitoring of bioprocess is important to ensure safety of a reactor and maintain high quality of products. It is difficult to build an accurate mechanistic model for a bioprocess, so fault monitoring based on rich historical or online database is an effective way. A group of data based on bootstrap method could be resampling stochastically, improving generalization capability of model. In this paper, online fault monitoring of generalized additive models （GAMs） combining with bootstrap is proposed for glutamate fermentation process. GAMs and bootstrap are first used to decide confidence interval based on the online and off-line normal sampled data from glutamate fermentation experiments. Then GAMs are used to online fault monitoring for time, dissolved oxygen, oxygen uptake rate, and carbon dioxide evolution rate. The method can provide accurate fault alarm online and is helpful to provide useful information for removing fault and abnormal phenomena in the fermentation.展开更多
《生物加工过程》(2003年创刊,双月刊)是由南京工业大学主办、中国生物工程学会和中国化工学会生物化工专业委员会协办、国内外公开发行的综合性中文学术期刊,国际刊号ISSN1672-3678,国内统一刊号CN32-1706/Q。本刊目前被《中国科技核...《生物加工过程》(2003年创刊,双月刊)是由南京工业大学主办、中国生物工程学会和中国化工学会生物化工专业委员会协办、国内外公开发行的综合性中文学术期刊,国际刊号ISSN1672-3678,国内统一刊号CN32-1706/Q。本刊目前被《中国科技核心期刊》、《科学引文数据库》(SCD)、《中国学术期刊综合评价数据库》《中国期刊全文数据库》《中国生物学文摘》、RCCSE中国核心学术期刊(A)、《万方数据资源系统数字化期刊》、台湾华艺思博网等中文数据库和美国《生物学文摘(预评)》(BP,BIOSIS Previews)、美国《化学文摘(网络版)》(CA)、英国《国际农业与生物科学研究中心》(CABI)、美国《剑桥科学文摘》(CSA)、波兰《哥白尼索引》(IC,Index of Copernicus)、美国《乌利希期刊指南(网络版)》(Ulrichsweb)等国外数据库收录。展开更多
基金Supported by the National Natural Science Foundation of China （20836004 20806046） the Special Fund for Major State Basic Research Program of China （2009CB724702） the National High Technology Research and Development Program ofChina （2009AA062903）
文摘Green fluorescent protein (GFP) and its variants /homolog proteins are generally called as GFP-like fluorescent proteins (FPs), which are widely used as visible molecular tools for monitoring a wide range of biological processes due to their capability of simple, accurate and real time quantification. The FPs-based molecular and visible quantification tools are giving more impact on bioprocess engineering, enabling the biomolecule-level dynamic information to be linked with the process-level events. In this review, different applications of FPs in biological engineering with emphasis on rapid molecular bioprocess quantification, such as quantification of the transcription efficiency, the protein production, the protein folding efficiency, the cell concentration, the intracellular microenvironments and so on, would be first introduced. The challenges of using FPs with respect to actual bioprocess applications for the precise quantification including the interaction of FPs and the fused partner proteins, the maturation of FPs, the inner filter effect and sensing technology were then discussed. Finally, the future development for the FPs used in molecular bioprocess quantification would be proposed.
基金Supported by the National Natural Science Foundation of China （61273131） 111 Project （B12018）+1 种基金 the Innovation Project of Graduate in Jiangsu Province （CXZZ12_0741） the Fundamental Research Funds for the Central Universities （JUDCF12034）
文摘Fault monitoring of bioprocess is important to ensure safety of a reactor and maintain high quality of products. It is difficult to build an accurate mechanistic model for a bioprocess, so fault monitoring based on rich historical or online database is an effective way. A group of data based on bootstrap method could be resampling stochastically, improving generalization capability of model. In this paper, online fault monitoring of generalized additive models （GAMs） combining with bootstrap is proposed for glutamate fermentation process. GAMs and bootstrap are first used to decide confidence interval based on the online and off-line normal sampled data from glutamate fermentation experiments. Then GAMs are used to online fault monitoring for time, dissolved oxygen, oxygen uptake rate, and carbon dioxide evolution rate. The method can provide accurate fault alarm online and is helpful to provide useful information for removing fault and abnormal phenomena in the fermentation.
文摘《生物加工过程》(2003年创刊,双月刊)是由南京工业大学主办、中国生物工程学会和中国化工学会生物化工专业委员会协办、国内外公开发行的综合性中文学术期刊,国际刊号ISSN1672-3678,国内统一刊号CN32-1706/Q。本刊目前被《中国科技核心期刊》、《科学引文数据库》(SCD)、《中国学术期刊综合评价数据库》《中国期刊全文数据库》《中国生物学文摘》、RCCSE中国核心学术期刊(A)、《万方数据资源系统数字化期刊》、台湾华艺思博网等中文数据库和美国《生物学文摘(预评)》(BP,BIOSIS Previews)、美国《化学文摘(网络版)》(CA)、英国《国际农业与生物科学研究中心》(CABI)、美国《剑桥科学文摘》(CSA)、波兰《哥白尼索引》(IC,Index of Copernicus)、美国《乌利希期刊指南(网络版)》(Ulrichsweb)等国外数据库收录。
