目的构建甘草黄酮类化合物系统性分离制备方法。方法采用特异性吸附材料富集甘草中的黄酮类化合物,以自主研发的制备色谱工厂系统,采用色谱分离专家系统软件优化分离制备条件,通过上样量和富集次数等参数的考察,建立了基于分离富集模式...目的构建甘草黄酮类化合物系统性分离制备方法。方法采用特异性吸附材料富集甘草中的黄酮类化合物,以自主研发的制备色谱工厂系统,采用色谱分离专家系统软件优化分离制备条件,通过上样量和富集次数等参数的考察,建立了基于分离富集模式的反相二维色谱制备甘草黄酮有效部位及单体化合物的方法。结果建立了以C18为分离、富集填料,甲醇-水、乙腈-水为一维、二维分离流动相,水为富集稀释液,梯度洗脱体积流量和稀释富集液的体积流量均为21 m L/min,上样量300 mg,富集次数3次的二维色谱分离制备甘草黄酮的方法,其分离过程具有良好的重复性。应用该方法分离制备,可重复获得16个甘草黄酮部位和甘草苷、甘草素、芒柄花黄素、刺甘草查耳酮、7,4′-二羟基黄酮、4′-O-[β-D-apio-D-furanosyl-(1→2)-β-D-glucopyranosyl]liquiritigenin、异甘草素、甘草酚、甘草香豆素共9个单体化合物。结论建立的甘草黄酮的制备方法为甘草资源的综合利用和甘草黄酮活性药物开发奠定了基础。展开更多
Aim To screen for α-glucosidase inhibitor from Glyeyrrhiza uralensis Fisch.. Methods Glycyrrhizic acid, glycyrrhetinic acid, flavonoids of glycyrrhiza, alkaloids of glycyrrhiza, and glycyrrhiza polysaccharides were i...Aim To screen for α-glucosidase inhibitor from Glyeyrrhiza uralensis Fisch.. Methods Glycyrrhizic acid, glycyrrhetinic acid, flavonoids of glycyrrhiza, alkaloids of glycyrrhiza, and glycyrrhiza polysaccharides were isolated from the root of Glycyrrhiza uralensis Fisch. respectively. Three compounds were isolated from the flavonoids of glycyrrhiza as guided by the α-glucosidase inhibitory test in vitro. Moreover, the characteristics of inhibitory kinetics of glycyrol and glycyrrhetinic acid were investi- gated. Results The flavonoids of glycyrrhiza and glycyrrhetinic acid had the strongest α-glucosidase inhibitory activity. Glycyrol,β-sitosterol and liquifitin were isolated and identified. Glycyrol was a fast- binding, reversible, noncompetitive α-glucosidase inhibitor, showing IC50 at 0.26 μg·mL^-1 Glycyrrhetinic acid was a fast-binding, irreversible α-glucosidase inhibitor, showing IC50 at 102.4 μg·mL^-1. Conclusion Glycyrol is an effective α-glucosidase inhibitor.展开更多
文摘目的构建甘草黄酮类化合物系统性分离制备方法。方法采用特异性吸附材料富集甘草中的黄酮类化合物,以自主研发的制备色谱工厂系统,采用色谱分离专家系统软件优化分离制备条件,通过上样量和富集次数等参数的考察,建立了基于分离富集模式的反相二维色谱制备甘草黄酮有效部位及单体化合物的方法。结果建立了以C18为分离、富集填料,甲醇-水、乙腈-水为一维、二维分离流动相,水为富集稀释液,梯度洗脱体积流量和稀释富集液的体积流量均为21 m L/min,上样量300 mg,富集次数3次的二维色谱分离制备甘草黄酮的方法,其分离过程具有良好的重复性。应用该方法分离制备,可重复获得16个甘草黄酮部位和甘草苷、甘草素、芒柄花黄素、刺甘草查耳酮、7,4′-二羟基黄酮、4′-O-[β-D-apio-D-furanosyl-(1→2)-β-D-glucopyranosyl]liquiritigenin、异甘草素、甘草酚、甘草香豆素共9个单体化合物。结论建立的甘草黄酮的制备方法为甘草资源的综合利用和甘草黄酮活性药物开发奠定了基础。
文摘Aim To screen for α-glucosidase inhibitor from Glyeyrrhiza uralensis Fisch.. Methods Glycyrrhizic acid, glycyrrhetinic acid, flavonoids of glycyrrhiza, alkaloids of glycyrrhiza, and glycyrrhiza polysaccharides were isolated from the root of Glycyrrhiza uralensis Fisch. respectively. Three compounds were isolated from the flavonoids of glycyrrhiza as guided by the α-glucosidase inhibitory test in vitro. Moreover, the characteristics of inhibitory kinetics of glycyrol and glycyrrhetinic acid were investi- gated. Results The flavonoids of glycyrrhiza and glycyrrhetinic acid had the strongest α-glucosidase inhibitory activity. Glycyrol,β-sitosterol and liquifitin were isolated and identified. Glycyrol was a fast- binding, reversible, noncompetitive α-glucosidase inhibitor, showing IC50 at 0.26 μg·mL^-1 Glycyrrhetinic acid was a fast-binding, irreversible α-glucosidase inhibitor, showing IC50 at 102.4 μg·mL^-1. Conclusion Glycyrol is an effective α-glucosidase inhibitor.
