A micellar capillary electrophoresis（MCE） method based on on-line sweeping technique was（developed） for a determination of trace quinolones,ciprofloxacin ofloxacin and enrofloxacin in pork tissue.The pork tissue s...A micellar capillary electrophoresis（MCE） method based on on-line sweeping technique was（developed） for a determination of trace quinolones,ciprofloxacin ofloxacin and enrofloxacin in pork tissue.The pork tissue samples were deproteinized with trichloroacetic acid and in the running buffer of 20 mmol/L（Na2B4O7）+80 mmol/L sodium dodecyl sulfate（SDS）,pH=9.6,the ciprofloxacin,ofloxacin and enrofloxacin in the sample were successfully separated and detected within 200 s,in a non-coating fused-silica capillary column（48.5 cm×75 μm i.d.,effective length 40 cm） at separation voltage of 18 kV,and the injection pressure of 15×102 Pa,with UV detection at 278 nm.The detection limits of ciprofloxacin,ofloxacin and enrofloxacin were 0.007,0.016 and 0.020 mg/L,and RSD were 2.0%,2.4% and 2.6%,respectively.This method allows of up to 600-fold accumulation concentration of ciprofloxacin and can be used to separate and detect the residuals of trace medicines in animal food.展开更多
为实现生物组织结构和成分变化的同时检测,提出体表内部虚拟超谱图(virtual internal hyperspec-trum of body surface,VIHBS)概念。在此基础上,设计了由调制光源、平移采集装置和光谱仪构成的VIH-BS采集系统。以猪肉为实验对象,等分为...为实现生物组织结构和成分变化的同时检测,提出体表内部虚拟超谱图(virtual internal hyperspec-trum of body surface,VIHBS)概念。在此基础上,设计了由调制光源、平移采集装置和光谱仪构成的VIH-BS采集系统。以猪肉为实验对象,等分为三份,一份为对照,剩余两份分别掺入红色滤光片和注入高散射物质脂肪乳溶液,模拟猪肉组织结构和成分的变化,并距入射光纤/mm处开始每隔0.5mm进行17个点的数据采集。结果表明,调制光源有效地抑制了环境光和光电器件暗电流影响,VIHBS技术在可见光波段实现了红色滤光片定位检测,以及在近红外波段的脂肪乳引起组织成分变化的筛查。这一研究表明VIHBS技术对组织内部结构和成分变化的检测有很大的可行性,随着研究深入有望为人体皮肤异常或疾病的早期诊断和筛查提供新手段。展开更多
文摘A micellar capillary electrophoresis（MCE） method based on on-line sweeping technique was（developed） for a determination of trace quinolones,ciprofloxacin ofloxacin and enrofloxacin in pork tissue.The pork tissue samples were deproteinized with trichloroacetic acid and in the running buffer of 20 mmol/L（Na2B4O7）+80 mmol/L sodium dodecyl sulfate（SDS）,pH=9.6,the ciprofloxacin,ofloxacin and enrofloxacin in the sample were successfully separated and detected within 200 s,in a non-coating fused-silica capillary column（48.5 cm×75 μm i.d.,effective length 40 cm） at separation voltage of 18 kV,and the injection pressure of 15×102 Pa,with UV detection at 278 nm.The detection limits of ciprofloxacin,ofloxacin and enrofloxacin were 0.007,0.016 and 0.020 mg/L,and RSD were 2.0%,2.4% and 2.6%,respectively.This method allows of up to 600-fold accumulation concentration of ciprofloxacin and can be used to separate and detect the residuals of trace medicines in animal food.
文摘为实现生物组织结构和成分变化的同时检测,提出体表内部虚拟超谱图(virtual internal hyperspec-trum of body surface,VIHBS)概念。在此基础上,设计了由调制光源、平移采集装置和光谱仪构成的VIH-BS采集系统。以猪肉为实验对象,等分为三份,一份为对照,剩余两份分别掺入红色滤光片和注入高散射物质脂肪乳溶液,模拟猪肉组织结构和成分的变化,并距入射光纤/mm处开始每隔0.5mm进行17个点的数据采集。结果表明,调制光源有效地抑制了环境光和光电器件暗电流影响,VIHBS技术在可见光波段实现了红色滤光片定位检测,以及在近红外波段的脂肪乳引起组织成分变化的筛查。这一研究表明VIHBS技术对组织内部结构和成分变化的检测有很大的可行性,随着研究深入有望为人体皮肤异常或疾病的早期诊断和筛查提供新手段。
