The purified recombinant nuclocapsid protein of PRRSV was used to conjoined with latex, and the conjoined concentration,temperature and time were optimized, a detection method for serum antibodies against Porcine repr...The purified recombinant nuclocapsid protein of PRRSV was used to conjoined with latex, and the conjoined concentration,temperature and time were optimized, a detection method for serum antibodies against Porcine reproductive and respiratory syndrome virus was established. Some tests for the method’s sensitivity, specificity and stability were confirmed. About 200 serum samples were detected by using the method, and these samples were also detected by recombinant N protein based ELISA and IDEXX ELISA kit. The result showed that the agreement ratio of the recombinant N protein based LAT with other two methods arrived at 93%, 78%, respectively.展开更多
猪繁殖与呼吸综合征(PRRS)于1987年首先发现于美国(Stevenson et al.,1993),以引起母猪流产、早产、产死胎、弱仔、木乃伊胎及诱发仔猪呼吸系统症状为主要特征的高度传染性病毒性疾病(Wensvoort,et al.,1991;Terpstra etal.,1991),是制...猪繁殖与呼吸综合征(PRRS)于1987年首先发现于美国(Stevenson et al.,1993),以引起母猪流产、早产、产死胎、弱仔、木乃伊胎及诱发仔猪呼吸系统症状为主要特征的高度传染性病毒性疾病(Wensvoort,et al.,1991;Terpstra etal.,1991),是制约养猪业发展的因素之一.该病在我国自1995年暴发此病使我国的养猪业蒙受重大经济损失(郭宝清等).PRRS同其它病毒性疾病一样,目前尚无有效的治疗性药物,控制该病主要方法仍是传统的免疫接种预防,国内外普遍使用常规疫苗即弱毒活疫苗和灭活疫苗,但是对上述两种PRRS疫苗的应用意见不一,为此笔者就猪繁殖与呼吸道综合征免疫预防做一介绍.展开更多
猪繁殖与呼吸综合征(porcine reporoductive and respiratory syndrome,PRRS)是严重危害养猪业的重要疾病之一。作者综述了近年来PRRS免疫学研究的最新进展,特别对细胞免疫、体液免疫、天然免疫、抗体依赖性增强作用等方面进行了深入的...猪繁殖与呼吸综合征(porcine reporoductive and respiratory syndrome,PRRS)是严重危害养猪业的重要疾病之一。作者综述了近年来PRRS免疫学研究的最新进展,特别对细胞免疫、体液免疫、天然免疫、抗体依赖性增强作用等方面进行了深入的论述,对参与该病免疫反应中相关因子引起的免疫病理学研究做了详细的介绍。展开更多
文摘The purified recombinant nuclocapsid protein of PRRSV was used to conjoined with latex, and the conjoined concentration,temperature and time were optimized, a detection method for serum antibodies against Porcine reproductive and respiratory syndrome virus was established. Some tests for the method’s sensitivity, specificity and stability were confirmed. About 200 serum samples were detected by using the method, and these samples were also detected by recombinant N protein based ELISA and IDEXX ELISA kit. The result showed that the agreement ratio of the recombinant N protein based LAT with other two methods arrived at 93%, 78%, respectively.
文摘猪繁殖与呼吸综合征(PRRS)于1987年首先发现于美国(Stevenson et al.,1993),以引起母猪流产、早产、产死胎、弱仔、木乃伊胎及诱发仔猪呼吸系统症状为主要特征的高度传染性病毒性疾病(Wensvoort,et al.,1991;Terpstra etal.,1991),是制约养猪业发展的因素之一.该病在我国自1995年暴发此病使我国的养猪业蒙受重大经济损失(郭宝清等).PRRS同其它病毒性疾病一样,目前尚无有效的治疗性药物,控制该病主要方法仍是传统的免疫接种预防,国内外普遍使用常规疫苗即弱毒活疫苗和灭活疫苗,但是对上述两种PRRS疫苗的应用意见不一,为此笔者就猪繁殖与呼吸道综合征免疫预防做一介绍.
文摘猪繁殖与呼吸综合征(porcine reporoductive and respiratory syndrome,PRRS)是严重危害养猪业的重要疾病之一。作者综述了近年来PRRS免疫学研究的最新进展,特别对细胞免疫、体液免疫、天然免疫、抗体依赖性增强作用等方面进行了深入的论述,对参与该病免疫反应中相关因子引起的免疫病理学研究做了详细的介绍。
