The gene library of Parocneria orienta nuclear polyhedrovirus(PaorNPV)was partly constructed by cloing some fragments of BamH I and EcoR I of PaorNPV genomic DNA into pGEM3Z(f-)as a vector.Using the chitinase gene of ...The gene library of Parocneria orienta nuclear polyhedrovirus(PaorNPV)was partly constructed by cloing some fragments of BamH I and EcoR I of PaorNPV genomic DNA into pGEM3Z(f-)as a vector.Using the chitinase gene of HearSNPV probed with[α- 32 P]dCTP,the chitinase gene of PaorNPV was localized on the fragments of BamH I-D,EcoR I-A,Hind Ⅲ-A,Pst I-D and Xho I-G/H respectively.At the same time,the fragment of BamH I-D containing the chiA has been cloned.展开更多
Bioassay of PoNPV+1%VBL,PoNPV+12.5 μL·L-1 Chlorbenzuron,PoNPV+15 μL·L-1 Trichlorphon was conducted on 2-instar larvae of Parocneria orientia in lab.The results showed that 1%VBL,12.5 μL·L-1 Chlorbenz...Bioassay of PoNPV+1%VBL,PoNPV+12.5 μL·L-1 Chlorbenzuron,PoNPV+15 μL·L-1 Trichlorphon was conducted on 2-instar larvae of Parocneria orientia in lab.The results showed that 1%VBL,12.5 μL·L-1 Chlorbenzuron,15 μL·L-1 Trichlorphon had better synergistic effect.Spraying of PoNPV+1%VBL,PoNPV+12.5 μL·L-1 Chlorbenzuron,PoNPV+15 μL·L-1 Trichlorphon and PoNPV to control 2～3 instar larvae of second generation in field,the concentrations of PoNPV were 8×106 PIB·mL-1,4×106 PIB·mL-1,2×106 PIB·mL-1,1×106 PIB·mL-1,5×105 PIB·mL-1,there were 20 treatments.It was indicated that when the concentration was the same,the effect of PoNPV added synergists was better than that without synergists.展开更多
LdMNPV-NEFU isolate collected from the forestry farm of Northeast Forestry University was purified and the genomic DNA of LdMNPV was extracted. The LdMNPV polyhedrin gene was cloned by PCR. The results showed that the...LdMNPV-NEFU isolate collected from the forestry farm of Northeast Forestry University was purified and the genomic DNA of LdMNPV was extracted. The LdMNPV polyhedrin gene was cloned by PCR. The results showed that the sequence was an open reading frame (ORF) of 735bp capable of encoding 245 amino acids. The polyhedrin gene sequences of the LdMNPV-NEFU isolate and a Canada strain, LdMNPV-G differed in 5 bases. The polyhedrin gene of the LdMNPV-NEFU isolate contained C, G, T, C and G at 54, 109,379, 508 and 701 sites from the start codon, but the LdMNPV-G isolate contained G, C, C, T and T at the corresponding sites respectively. The same amino acids were encoded by the two ORF sequences, with the exception that Asp and His are encoded by GAC on the polyhedrin gene sequence of the LdMNPV-NEFU isolate and by CAC in the LdMNPV-G isolate. The LdMNPV polyhedrin gene was expressed in E.coli BL21 (DE3) by the pT7-7 plasmid vector.展开更多
文摘The gene library of Parocneria orienta nuclear polyhedrovirus(PaorNPV)was partly constructed by cloing some fragments of BamH I and EcoR I of PaorNPV genomic DNA into pGEM3Z(f-)as a vector.Using the chitinase gene of HearSNPV probed with[α- 32 P]dCTP,the chitinase gene of PaorNPV was localized on the fragments of BamH I-D,EcoR I-A,Hind Ⅲ-A,Pst I-D and Xho I-G/H respectively.At the same time,the fragment of BamH I-D containing the chiA has been cloned.
文摘Bioassay of PoNPV+1%VBL,PoNPV+12.5 μL·L-1 Chlorbenzuron,PoNPV+15 μL·L-1 Trichlorphon was conducted on 2-instar larvae of Parocneria orientia in lab.The results showed that 1%VBL,12.5 μL·L-1 Chlorbenzuron,15 μL·L-1 Trichlorphon had better synergistic effect.Spraying of PoNPV+1%VBL,PoNPV+12.5 μL·L-1 Chlorbenzuron,PoNPV+15 μL·L-1 Trichlorphon and PoNPV to control 2～3 instar larvae of second generation in field,the concentrations of PoNPV were 8×106 PIB·mL-1,4×106 PIB·mL-1,2×106 PIB·mL-1,1×106 PIB·mL-1,5×105 PIB·mL-1,there were 20 treatments.It was indicated that when the concentration was the same,the effect of PoNPV added synergists was better than that without synergists.
文摘LdMNPV-NEFU isolate collected from the forestry farm of Northeast Forestry University was purified and the genomic DNA of LdMNPV was extracted. The LdMNPV polyhedrin gene was cloned by PCR. The results showed that the sequence was an open reading frame (ORF) of 735bp capable of encoding 245 amino acids. The polyhedrin gene sequences of the LdMNPV-NEFU isolate and a Canada strain, LdMNPV-G differed in 5 bases. The polyhedrin gene of the LdMNPV-NEFU isolate contained C, G, T, C and G at 54, 109,379, 508 and 701 sites from the start codon, but the LdMNPV-G isolate contained G, C, C, T and T at the corresponding sites respectively. The same amino acids were encoded by the two ORF sequences, with the exception that Asp and His are encoded by GAC on the polyhedrin gene sequence of the LdMNPV-NEFU isolate and by CAC in the LdMNPV-G isolate. The LdMNPV polyhedrin gene was expressed in E.coli BL21 (DE3) by the pT7-7 plasmid vector.
