Pitavastatin belongs to the class of coenzyme A reductase inhibitors.Very few methods of assaying pitavastatin from human plasma are available in literature.An analytical method is presented for the determination of t...Pitavastatin belongs to the class of coenzyme A reductase inhibitors.Very few methods of assaying pitavastatin from human plasma are available in literature.An analytical method is presented for the determination of the drug from human plasma making use of the fluorescent property of the drug.The drug is extracted from plasma using ethyl acetate under neutral condition and then analyzed by reversed-phase high performance liquid chromatography(HPLC) with fluorescence detection(λEx 245 nm;λEm 420 nm).Analysis of pitavastatin was carried out on a C18 HPLC column using a gradient flow of mobile phase(0.01 mol/L monobasic potassium phosphate(pH 3.20)-acetonitrile,63:37,v/v).Fluorescein isothiocyanate was used as internal standard.The dynamic range of assay was 3 to 50 ng/mL.The intraday precision was less than 10% and accuracy ranged from 95.2% to 112.6%.The same for interday check was less than 12% and 92.8% to 105.1%,respectively.The drug was found to be stable under the assay conditions.The developed method is simple,precise,accurate,and stable.This indicates that it can be applied to routine analysis of this drug in human subjects where there are large numbers of samples without the need of specialized instruments like column switching.展开更多
连续侵入性信号扩大反应(the serial invasive signal amplification reac-tion,SISAR)不同于以前的 Third Wave Technologies 公司推出的侵入信号扩大反应,它应用两个连续的侵入反应在恒温系统中将靶位点的信号放大,并通过激发荧光来...连续侵入性信号扩大反应(the serial invasive signal amplification reac-tion,SISAR)不同于以前的 Third Wave Technologies 公司推出的侵入信号扩大反应,它应用两个连续的侵入反应在恒温系统中将靶位点的信号放大,并通过激发荧光来显示信号。具有较高的灵敏性和严紧度,是不需要热循环 PCR 反应和限制性酶切反应的一种另类序列检测手段。展开更多
文摘Pitavastatin belongs to the class of coenzyme A reductase inhibitors.Very few methods of assaying pitavastatin from human plasma are available in literature.An analytical method is presented for the determination of the drug from human plasma making use of the fluorescent property of the drug.The drug is extracted from plasma using ethyl acetate under neutral condition and then analyzed by reversed-phase high performance liquid chromatography(HPLC) with fluorescence detection(λEx 245 nm;λEm 420 nm).Analysis of pitavastatin was carried out on a C18 HPLC column using a gradient flow of mobile phase(0.01 mol/L monobasic potassium phosphate(pH 3.20)-acetonitrile,63:37,v/v).Fluorescein isothiocyanate was used as internal standard.The dynamic range of assay was 3 to 50 ng/mL.The intraday precision was less than 10% and accuracy ranged from 95.2% to 112.6%.The same for interday check was less than 12% and 92.8% to 105.1%,respectively.The drug was found to be stable under the assay conditions.The developed method is simple,precise,accurate,and stable.This indicates that it can be applied to routine analysis of this drug in human subjects where there are large numbers of samples without the need of specialized instruments like column switching.
文摘连续侵入性信号扩大反应(the serial invasive signal amplification reac-tion,SISAR)不同于以前的 Third Wave Technologies 公司推出的侵入信号扩大反应,它应用两个连续的侵入反应在恒温系统中将靶位点的信号放大,并通过激发荧光来显示信号。具有较高的灵敏性和严紧度,是不需要热循环 PCR 反应和限制性酶切反应的一种另类序列检测手段。
