Adding sodium chloride into a medium containing 140g·L - 1 glucose could promote the glycerol accumulation efficiently, but the glycero l productivity would decrease when sodium chloride concentration was above 4...Adding sodium chloride into a medium containing 140g·L - 1 glucose could promote the glycerol accumulation efficiently, but the glycero l productivity would decrease when sodium chloride concentration was above 40g· L -1.Experimental results in shake flask showed that sodium chloride conce ntration of 40g·L -1 was favorable.Moreover, the feasibility of multi-st age cascade continuous fermentation was tested and the results indicated that mu lti-stage continuous system was superior to single-stage continuous fermentati on.Compared with batch fermentation,the glycerol concentration and the glycerol yield in multi-stage cascade continuous system decreased by only 9.83 % and 5 .71 % respectively, but the glycerol productivity increased by 18.54% even the preparing time between two batch fermentation was not considered..展开更多
Candida krusei was osmotolerant yeast for the production of glycerol. Extracellular osmotic pressure was one of the key factors to induce the enzyme activity of glycerol-3-phosphate dehydrogenase（GPDH） which severel...Candida krusei was osmotolerant yeast for the production of glycerol. Extracellular osmotic pressure was one of the key factors to induce the enzyme activity of glycerol-3-phosphate dehydrogenase（GPDH） which severely affected the glycerol productivity. Three osmoregulators such as NaCl, PEG4000 and glycerol were used to investigate their effects on yeast growth, glucose consumption and glycerol production. In order to determine the effect of extracellular glycerol concentration, different amounts of glycerol were initially supplemented as an osmoregulator to increase glycerol production. The maximum glycerol concentration attained 179g·L^-1 with initial glycerol concentration of 80g·L^-1 in medium, compared with 41g·L^-1 in the control experiment. These results were successfully reoroduced for fed-batch orocess in an air-lift reactor.展开更多
文摘Adding sodium chloride into a medium containing 140g·L - 1 glucose could promote the glycerol accumulation efficiently, but the glycero l productivity would decrease when sodium chloride concentration was above 40g· L -1.Experimental results in shake flask showed that sodium chloride conce ntration of 40g·L -1 was favorable.Moreover, the feasibility of multi-st age cascade continuous fermentation was tested and the results indicated that mu lti-stage continuous system was superior to single-stage continuous fermentati on.Compared with batch fermentation,the glycerol concentration and the glycerol yield in multi-stage cascade continuous system decreased by only 9.83 % and 5 .71 % respectively, but the glycerol productivity increased by 18.54% even the preparing time between two batch fermentation was not considered..
基金Supported by the National Natural Science Foundation of China （No.20576118）.
文摘Candida krusei was osmotolerant yeast for the production of glycerol. Extracellular osmotic pressure was one of the key factors to induce the enzyme activity of glycerol-3-phosphate dehydrogenase（GPDH） which severely affected the glycerol productivity. Three osmoregulators such as NaCl, PEG4000 and glycerol were used to investigate their effects on yeast growth, glucose consumption and glycerol production. In order to determine the effect of extracellular glycerol concentration, different amounts of glycerol were initially supplemented as an osmoregulator to increase glycerol production. The maximum glycerol concentration attained 179g·L^-1 with initial glycerol concentration of 80g·L^-1 in medium, compared with 41g·L^-1 in the control experiment. These results were successfully reoroduced for fed-batch orocess in an air-lift reactor.
