白细胞介素2(Inter Leu kin2 IL-2)又称T细胞生长因子(TCGF)。是T淋巴细胞受抗原刺激后所分泌的具有免疫调节作用的淋巴因子。IL-2诱导自然杀伤细胞(NK)、细胞毒性T细胞(CTL)、淋巴因子活化的杀伤细胞(LAK)的活化和增殖．从而杀伤肿瘤...白细胞介素2(Inter Leu kin2 IL-2)又称T细胞生长因子(TCGF)。是T淋巴细胞受抗原刺激后所分泌的具有免疫调节作用的淋巴因子。IL-2诱导自然杀伤细胞(NK)、细胞毒性T细胞(CTL)、淋巴因子活化的杀伤细胞(LAK)的活化和增殖．从而杀伤肿瘤细胞的作用近年来日益受到人们的重视。展开更多
现年51岁的史蒂文·罗森伯格(Steven A. Rosenberg)目前是美国国立癌症研究所外科主任。还是在遥远的孩提时代,罗森伯格便立志长大要当一名医生。他曾经这样描述自己对从事医学工作的抱负:“我希望能亲自照看病人,但是我并不打算循...现年51岁的史蒂文·罗森伯格(Steven A. Rosenberg)目前是美国国立癌症研究所外科主任。还是在遥远的孩提时代,罗森伯格便立志长大要当一名医生。他曾经这样描述自己对从事医学工作的抱负:“我希望能亲自照看病人,但是我并不打算循规蹈距地按今天的方法去行医,我的理想是为发展明天的医学事业而有所作为。”史蒂文·罗森伯格出生于1940年8月2日。展开更多
AIM: To improve the preparation of adherent lymphokine-activated killer (A-LAK) cells and to study the effects ofcryopreservation and phenylacetate (PA) on biologicalcharacters of A-LAK cells.METHODS: A-LAK cells were...AIM: To improve the preparation of adherent lymphokine-activated killer (A-LAK) cells and to study the effects ofcryopreservation and phenylacetate (PA) on biologicalcharacters of A-LAK cells.METHODS: A-LAK cells were obtained from peripheral bloodmononuclear cells (PBMCs) of the patients withhepatocellular caminoma (HCC) by using L-phenylalaninemethyl ester ( PME ) to deplete immunosuppressivermonocytes. Proliferative activity of SMMC7721 cell line aftertreatment with phenylacetate (PA) was observed. A-LAKcells were treated with the supernatant of SMMC7721 cellsthat had been pretreated with PA. The changes ofproliferation, cytotoxicity and phenotype of A-LAK cellswere investigated after cryopreservstion.RESULTS: The expansion of A-LAK cells (96.79± 69.10 foldson Day 14 ) was significantly higher then that of non-adherent LAK (NA-LAK) cells (22.77 ± 13.20) as well asconventional LAK cells (4. 64 ± 0. 91 ). PA significantlysuppressed the growth of SMMC7721 cells, and the inhibitorratio was 46 %. rite supernatant of cultured tumor cellsintensively suppressed the proliferation and cytotoxicity ofA-LAK cells, but the suppressive effect of the supernatantwas previously decreased after treatment with PA.Impairments in proliferation and cytotoxicity of A-LAK cellsimmediately after thawing of cryopreservation and recoveryafter reincubation with IL-2 were observed. The cytotoxicltyof thawed A-LAK cells on Day 5 was significantly higher thanthat of fresh A-LAK before freezing (54.8 ± 10.2 % vs 40.5 ±6. 4 %). No significant change in the percentage oflymphocyte subsets was identified in frozen A-LAK cells ascompared with that in the fresh control cells.CONCLUSION: A-LAK cells can he simply prepared by usingPME, and showed a synergistic anti-tumor effect with thecombination of PA. Cryopreseration can increase theimmunoactivities of A-LAK cells from the patients withhepatocellular caminoma .展开更多
文摘白细胞介素2(Inter Leu kin2 IL-2)又称T细胞生长因子(TCGF)。是T淋巴细胞受抗原刺激后所分泌的具有免疫调节作用的淋巴因子。IL-2诱导自然杀伤细胞(NK)、细胞毒性T细胞(CTL)、淋巴因子活化的杀伤细胞(LAK)的活化和增殖．从而杀伤肿瘤细胞的作用近年来日益受到人们的重视。
文摘现年51岁的史蒂文·罗森伯格(Steven A. Rosenberg)目前是美国国立癌症研究所外科主任。还是在遥远的孩提时代,罗森伯格便立志长大要当一名医生。他曾经这样描述自己对从事医学工作的抱负:“我希望能亲自照看病人,但是我并不打算循规蹈距地按今天的方法去行医,我的理想是为发展明天的医学事业而有所作为。”史蒂文·罗森伯格出生于1940年8月2日。
基金the National 9th Five-Year Program of China,No.96-906-01-20
文摘AIM: To improve the preparation of adherent lymphokine-activated killer (A-LAK) cells and to study the effects ofcryopreservation and phenylacetate (PA) on biologicalcharacters of A-LAK cells.METHODS: A-LAK cells were obtained from peripheral bloodmononuclear cells (PBMCs) of the patients withhepatocellular caminoma (HCC) by using L-phenylalaninemethyl ester ( PME ) to deplete immunosuppressivermonocytes. Proliferative activity of SMMC7721 cell line aftertreatment with phenylacetate (PA) was observed. A-LAKcells were treated with the supernatant of SMMC7721 cellsthat had been pretreated with PA. The changes ofproliferation, cytotoxicity and phenotype of A-LAK cellswere investigated after cryopreservstion.RESULTS: The expansion of A-LAK cells (96.79± 69.10 foldson Day 14 ) was significantly higher then that of non-adherent LAK (NA-LAK) cells (22.77 ± 13.20) as well asconventional LAK cells (4. 64 ± 0. 91 ). PA significantlysuppressed the growth of SMMC7721 cells, and the inhibitorratio was 46 %. rite supernatant of cultured tumor cellsintensively suppressed the proliferation and cytotoxicity ofA-LAK cells, but the suppressive effect of the supernatantwas previously decreased after treatment with PA.Impairments in proliferation and cytotoxicity of A-LAK cellsimmediately after thawing of cryopreservation and recoveryafter reincubation with IL-2 were observed. The cytotoxicltyof thawed A-LAK cells on Day 5 was significantly higher thanthat of fresh A-LAK before freezing (54.8 ± 10.2 % vs 40.5 ±6. 4 %). No significant change in the percentage oflymphocyte subsets was identified in frozen A-LAK cells ascompared with that in the fresh control cells.CONCLUSION: A-LAK cells can he simply prepared by usingPME, and showed a synergistic anti-tumor effect with thecombination of PA. Cryopreseration can increase theimmunoactivities of A-LAK cells from the patients withhepatocellular caminoma .
