The major metabolites of ginsenoside-Rb1 in rats urine were described.Urine samples were collected before and after 24 h of single oral administration of 150 mg and intravenous administration of 60 mg Rb1 to four rats...The major metabolites of ginsenoside-Rb1 in rats urine were described.Urine samples were collected before and after 24 h of single oral administration of 150 mg and intravenous administration of 60 mg Rb1 to four rats,respectively.The samples were purified by SPE column and then were analyzed by liquid chromatography-ESI-mass spectrometry for potential metabolites.Parent drug and its fourteen metabolites were identified in rat urine based on the comparison of total ion chromatograms of the blank with the metabolic urine as well as mass spectra.Its main metabolic pathways and possible structures are analyzed in detail.展开更多
采用胶体金免疫层析试纸条法与高效液相色谱-飞行时间串联质谱法(High performance liquid chromatography-quadrupole time of flight-mass spectrometry,HPLC-QTOF-MS)检测保健品中的那非类药物,并对这两种方法进行了比较.结果表明:...采用胶体金免疫层析试纸条法与高效液相色谱-飞行时间串联质谱法(High performance liquid chromatography-quadrupole time of flight-mass spectrometry,HPLC-QTOF-MS)检测保健品中的那非类药物,并对这两种方法进行了比较.结果表明:胶体金免疫层析试纸条法对豪莫西地那非的检测限为30μg/mL,对硫代艾地那非、红地那非、那红地那非及伪伐地那非的检测限均为60μg/mL;HPLC-QTOF-MS法对豪莫西地那非、硫代艾地那非、红地那非、那红地那非、伪伐地那非的检测限为0.2μg/mL.两种方法对实际样品的定性检测结果一致.与HPLC-QTOF-MS相比,胶体金免疫层析试纸条具有操作简便、快速、直接的特点,可作为样品的快速定性筛查,HPLC-QTOF-MS法可作为阳性样品的进一步确证和精确定量.展开更多
目的:系统分析黄甲软肝颗粒及其中间体的化学成分,阐明其复方新药的化学物质基础。方法:采用超高效液相色谱-飞行时间串联质谱(UHPLC-Q-TOF-MS)技术,离子源为ESI,色谱条件:Agilent Zorbax Eclipse Plus C_(18)色谱柱(2.1mm×150mm,...目的:系统分析黄甲软肝颗粒及其中间体的化学成分,阐明其复方新药的化学物质基础。方法:采用超高效液相色谱-飞行时间串联质谱(UHPLC-Q-TOF-MS)技术,离子源为ESI,色谱条件:Agilent Zorbax Eclipse Plus C_(18)色谱柱(2.1mm×150mm,1.8μm),流动相为0.1%甲酸水溶液(A)-0.1%甲酸乙腈(B),洗脱梯度:0~20min,3%~60%B;20min~25min,60%~95%B;25min~30min,95%B;30min~31min,95%~3%B;31min~35min,3%B,流速0.35mL·min^(-1),柱温35℃,进样量1μL。质谱分析采用正、负离子模式扫描,扫描范围m/z100~1700。通过与自建化学成分数据库比对(部分对照品验证)后对各离子峰进行鉴定。结果:从黄甲软肝颗粒中共鉴定出126个化学成分,黄甲软肝中间体中共鉴定出141个化学成分,包括有机酸类、酚酸类、黄酮类、三萜类、皂苷类等。结论:建立的UHPLC-TOF-MS分析方法,结合数据库匹配,可全面快速地分析黄甲软肝颗粒及中间体中的化学成分,为其组方药效物质基础及质量控制研究提供科学依据。展开更多
采用超高效液相色谱-四极杆-飞行时间串联质谱(ultra-high performance liquid chromatography coupled with quadrupole/time-of-flight tandem mass spectrometry,UPLC-Q/TOF-MS/MS)技术,对夏佛塔苷口服给药后在小鼠体内的原形成分及...采用超高效液相色谱-四极杆-飞行时间串联质谱(ultra-high performance liquid chromatography coupled with quadrupole/time-of-flight tandem mass spectrometry,UPLC-Q/TOF-MS/MS)技术,对夏佛塔苷口服给药后在小鼠体内的原形成分及其代谢产物进行定性分析。动物福利和实验过程获得徐州医科大学伦理委员会的批准(批准号:XZMULL201612024)。通过分析各成分的精确分子质量、一级、二级质谱信息,并与相应的对照品及文献比对,从小鼠血浆、胆汁、尿液和粪便中共鉴定出1个原形成分和29个代谢产物。这些成分在小鼠体内代谢的主要途径有去羟基化、羟基化、脱糖化、糖化、氢化、甲基化、乙酰化、硫酸化、葡糖醛酸化等。研究结果可为进一步阐明夏佛塔苷的药效物质基础提供有价值的依据。展开更多
文摘The major metabolites of ginsenoside-Rb1 in rats urine were described.Urine samples were collected before and after 24 h of single oral administration of 150 mg and intravenous administration of 60 mg Rb1 to four rats,respectively.The samples were purified by SPE column and then were analyzed by liquid chromatography-ESI-mass spectrometry for potential metabolites.Parent drug and its fourteen metabolites were identified in rat urine based on the comparison of total ion chromatograms of the blank with the metabolic urine as well as mass spectra.Its main metabolic pathways and possible structures are analyzed in detail.
文摘采用胶体金免疫层析试纸条法与高效液相色谱-飞行时间串联质谱法(High performance liquid chromatography-quadrupole time of flight-mass spectrometry,HPLC-QTOF-MS)检测保健品中的那非类药物,并对这两种方法进行了比较.结果表明:胶体金免疫层析试纸条法对豪莫西地那非的检测限为30μg/mL,对硫代艾地那非、红地那非、那红地那非及伪伐地那非的检测限均为60μg/mL;HPLC-QTOF-MS法对豪莫西地那非、硫代艾地那非、红地那非、那红地那非、伪伐地那非的检测限为0.2μg/mL.两种方法对实际样品的定性检测结果一致.与HPLC-QTOF-MS相比,胶体金免疫层析试纸条具有操作简便、快速、直接的特点,可作为样品的快速定性筛查,HPLC-QTOF-MS法可作为阳性样品的进一步确证和精确定量.
文摘目的:系统分析黄甲软肝颗粒及其中间体的化学成分,阐明其复方新药的化学物质基础。方法:采用超高效液相色谱-飞行时间串联质谱(UHPLC-Q-TOF-MS)技术,离子源为ESI,色谱条件:Agilent Zorbax Eclipse Plus C_(18)色谱柱(2.1mm×150mm,1.8μm),流动相为0.1%甲酸水溶液(A)-0.1%甲酸乙腈(B),洗脱梯度:0~20min,3%~60%B;20min~25min,60%~95%B;25min~30min,95%B;30min~31min,95%~3%B;31min~35min,3%B,流速0.35mL·min^(-1),柱温35℃,进样量1μL。质谱分析采用正、负离子模式扫描,扫描范围m/z100~1700。通过与自建化学成分数据库比对(部分对照品验证)后对各离子峰进行鉴定。结果:从黄甲软肝颗粒中共鉴定出126个化学成分,黄甲软肝中间体中共鉴定出141个化学成分,包括有机酸类、酚酸类、黄酮类、三萜类、皂苷类等。结论:建立的UHPLC-TOF-MS分析方法,结合数据库匹配,可全面快速地分析黄甲软肝颗粒及中间体中的化学成分,为其组方药效物质基础及质量控制研究提供科学依据。
文摘采用超高效液相色谱-四极杆-飞行时间串联质谱(ultra-high performance liquid chromatography coupled with quadrupole/time-of-flight tandem mass spectrometry,UPLC-Q/TOF-MS/MS)技术,对夏佛塔苷口服给药后在小鼠体内的原形成分及其代谢产物进行定性分析。动物福利和实验过程获得徐州医科大学伦理委员会的批准(批准号:XZMULL201612024)。通过分析各成分的精确分子质量、一级、二级质谱信息,并与相应的对照品及文献比对,从小鼠血浆、胆汁、尿液和粪便中共鉴定出1个原形成分和29个代谢产物。这些成分在小鼠体内代谢的主要途径有去羟基化、羟基化、脱糖化、糖化、氢化、甲基化、乙酰化、硫酸化、葡糖醛酸化等。研究结果可为进一步阐明夏佛塔苷的药效物质基础提供有价值的依据。
