The contents of total phenolics, flavonoid and chlorogenic acid in two types of sand pear fruits 〔\%Pyrus pyrifolia \%(Burm. f.) Nakai〕, and antioxidant activities in its juice were analysed. The results showed that...The contents of total phenolics, flavonoid and chlorogenic acid in two types of sand pear fruits 〔\%Pyrus pyrifolia \%(Burm. f.) Nakai〕, and antioxidant activities in its juice were analysed. The results showed that the contents of total phenolics and flavonoid in fruit of black sand pear were higher than that of red sand pear fruit obviously, but chlorogenic acid contents were not different in two types of sand pear fruit. The CI\-\{50\}of scavenging ·OH of their juice were 30.6 μL and 69.5 μL respectively. And their juice could inhibit lipid peroxidation significantly in brain and liver homogenates of rat. These implied that sand pear fruits have large quantity of antioxidant matters and were worthy to be further researched.展开更多
‘Shinseiki’ pear, a superior cultivars of Pyrus pyrifolia, was bred from a cross of ‘Nijisseiki’× ‘Chojuro’ in Japan. In China, many excellent pear cultivars have been released using ‘Shinseiki’ as a pare...‘Shinseiki’ pear, a superior cultivars of Pyrus pyrifolia, was bred from a cross of ‘Nijisseiki’× ‘Chojuro’ in Japan. In China, many excellent pear cultivars have been released using ‘Shinseiki’ as a parent. In this study, the S-genotypes of 13 progenies of ‘Shinseiki’ were identified by PCR-RFLP analysis, DNA sequencing and bioinformatic method. As a control, ‘Shinseiki’ was also included. PCR amplification showed that a common fragment of about 370 bp was generated in all the 13 cultivars. Restriction digestion analysis showed that PCR product amplified from ten cultivars ‘Huangguan’, ‘Yaqing’, ‘Bishanerhao’, ‘Xinya’, ‘Cuilv’, ‘Xizilv’, ‘Zhonglierhao’, ‘Zhongliyihao’, ‘Qingxiang’ and ‘Qinghua’ could be cut by S4-allele-specific endonuclease, and that from ‘Xinhang’, ‘Cuiguan’ and ‘Zaomeisu’ could be cut by S3-allele-specific endonuclease. DNA sequencing and bioinformatic analysis revealed that the undigested PCR fragment of ‘Huangguan’, ‘Cuiguan’, ‘Zaomeisu’, ‘Yaqing’, ‘Bishanerhao’, ‘Xinhang’, ‘Cuilv’, ‘Xizilv’, ‘Zhonglierhao’, ‘Zhongliyihao’, ‘Qingxiang’, ‘Xinya’ and ‘Qinghua’ was identical to S 16 , S5, S 35 , S 17 , S 16 , S1, S3, S1, S 31 , S 35 , S7, S 17 and S1, respectively. Consequently, the S-genotypes of 13 cultivars were as follows: ‘Huangguan’ (S4S 16 ), ‘Yaqing’ (S4S 17 ), ‘Xinhang’ (S1S3), ‘Bishanerhao’ (S4S 16 ), ‘Xinya’ (S4S 17 ), ‘Cuilv’ (S3S4), ‘Xizilv’ (S1S4), ‘Zhonglierhao’ (S4S 31 ), ‘Zhongliyihao’ (S4S 35 ), ‘Zaomeisu’ (S3S 35 ), ‘Cuiguan’ (S3S5), ‘Qingxiang’ (S4S7) and ‘Qinghua’ (S1S4).展开更多
文摘The contents of total phenolics, flavonoid and chlorogenic acid in two types of sand pear fruits 〔\%Pyrus pyrifolia \%(Burm. f.) Nakai〕, and antioxidant activities in its juice were analysed. The results showed that the contents of total phenolics and flavonoid in fruit of black sand pear were higher than that of red sand pear fruit obviously, but chlorogenic acid contents were not different in two types of sand pear fruit. The CI\-\{50\}of scavenging ·OH of their juice were 30.6 μL and 69.5 μL respectively. And their juice could inhibit lipid peroxidation significantly in brain and liver homogenates of rat. These implied that sand pear fruits have large quantity of antioxidant matters and were worthy to be further researched.
文摘‘Shinseiki’ pear, a superior cultivars of Pyrus pyrifolia, was bred from a cross of ‘Nijisseiki’× ‘Chojuro’ in Japan. In China, many excellent pear cultivars have been released using ‘Shinseiki’ as a parent. In this study, the S-genotypes of 13 progenies of ‘Shinseiki’ were identified by PCR-RFLP analysis, DNA sequencing and bioinformatic method. As a control, ‘Shinseiki’ was also included. PCR amplification showed that a common fragment of about 370 bp was generated in all the 13 cultivars. Restriction digestion analysis showed that PCR product amplified from ten cultivars ‘Huangguan’, ‘Yaqing’, ‘Bishanerhao’, ‘Xinya’, ‘Cuilv’, ‘Xizilv’, ‘Zhonglierhao’, ‘Zhongliyihao’, ‘Qingxiang’ and ‘Qinghua’ could be cut by S4-allele-specific endonuclease, and that from ‘Xinhang’, ‘Cuiguan’ and ‘Zaomeisu’ could be cut by S3-allele-specific endonuclease. DNA sequencing and bioinformatic analysis revealed that the undigested PCR fragment of ‘Huangguan’, ‘Cuiguan’, ‘Zaomeisu’, ‘Yaqing’, ‘Bishanerhao’, ‘Xinhang’, ‘Cuilv’, ‘Xizilv’, ‘Zhonglierhao’, ‘Zhongliyihao’, ‘Qingxiang’, ‘Xinya’ and ‘Qinghua’ was identical to S 16 , S5, S 35 , S 17 , S 16 , S1, S3, S1, S 31 , S 35 , S7, S 17 and S1, respectively. Consequently, the S-genotypes of 13 cultivars were as follows: ‘Huangguan’ (S4S 16 ), ‘Yaqing’ (S4S 17 ), ‘Xinhang’ (S1S3), ‘Bishanerhao’ (S4S 16 ), ‘Xinya’ (S4S 17 ), ‘Cuilv’ (S3S4), ‘Xizilv’ (S1S4), ‘Zhonglierhao’ (S4S 31 ), ‘Zhongliyihao’ (S4S 35 ), ‘Zaomeisu’ (S3S 35 ), ‘Cuiguan’ (S3S5), ‘Qingxiang’ (S4S7) and ‘Qinghua’ (S1S4).