[Objective] The research aimed to study the rapid propagation technology and establish effective clone of Hemistepta lyrata Bunge. [Method] With tender stem of Hemistepta lyrata Bunge as material, the conditions neede...[Objective] The research aimed to study the rapid propagation technology and establish effective clone of Hemistepta lyrata Bunge. [Method] With tender stem of Hemistepta lyrata Bunge as material, the conditions needed in calluses induction and differentiation, adventitious bud differentiation and radication, test tube seedling cutting and transplantation were studied. [ Result] The results showed that the optimum medium for granulated calluses induction from tender stem was MS + BA 0.3 mg/L +2,4-D 1 -1.5 mg/L, for granulated calluses and adventitious bud differentiation was MS + AgNO31.5 mg/L + BA 0.4 mg/L + NAA 0.1 mg/L. 1/2 MS + IAA 0.6 mg/L was suitable for test tube seedling rooting and regeneration, and cinder was used as transplantation and cutting substrate. [ Conclusion]This study will provide the scientific reference for choosing the feasible medium in tissue culture of Hemistepta lyrata Bunge.展开更多
Brine,which is used to produce high-purity magnesia,was purified by XSC-700 to remove boron.Boron adsorption capacity of XSC-700 was investigated by varying the initial boron concentration,temperature,resin/brine rati...Brine,which is used to produce high-purity magnesia,was purified by XSC-700 to remove boron.Boron adsorption capacity of XSC-700 was investigated by varying the initial boron concentration,temperature,resin/brine ratio and stirring speed,while keeping the diameter at constant.The results show that boron adsorption capacity increases with increasing boron concentration,temperature,and decreases with increasing resin/brine ratio.And the stirring speed could slightly affect the boron adsorption capacity.The adsorption kinetics obeys the pseudo-second-order model.Equilibrium data were both examined by Freundlich and Langmuir isotherm equations and it can be well represented by the Freundlich isotherm equation.展开更多
文摘[Objective] The research aimed to study the rapid propagation technology and establish effective clone of Hemistepta lyrata Bunge. [Method] With tender stem of Hemistepta lyrata Bunge as material, the conditions needed in calluses induction and differentiation, adventitious bud differentiation and radication, test tube seedling cutting and transplantation were studied. [ Result] The results showed that the optimum medium for granulated calluses induction from tender stem was MS + BA 0.3 mg/L +2,4-D 1 -1.5 mg/L, for granulated calluses and adventitious bud differentiation was MS + AgNO31.5 mg/L + BA 0.4 mg/L + NAA 0.1 mg/L. 1/2 MS + IAA 0.6 mg/L was suitable for test tube seedling rooting and regeneration, and cinder was used as transplantation and cutting substrate. [ Conclusion]This study will provide the scientific reference for choosing the feasible medium in tissue culture of Hemistepta lyrata Bunge.
基金Project(2008BAB35B04) supported by the National Key Technology R&D Program of China
文摘Brine,which is used to produce high-purity magnesia,was purified by XSC-700 to remove boron.Boron adsorption capacity of XSC-700 was investigated by varying the initial boron concentration,temperature,resin/brine ratio and stirring speed,while keeping the diameter at constant.The results show that boron adsorption capacity increases with increasing boron concentration,temperature,and decreases with increasing resin/brine ratio.And the stirring speed could slightly affect the boron adsorption capacity.The adsorption kinetics obeys the pseudo-second-order model.Equilibrium data were both examined by Freundlich and Langmuir isotherm equations and it can be well represented by the Freundlich isotherm equation.