目的晚期氧化蛋白质终产物(Advanced Oxidation Protein Products,AOPPs)是一种新型炎症介质,参与多种疾病的发生、发展。本研究探讨AOPPs对老年大鼠骨密度和骨微结构的影响。方法 70只18月龄雄性SD大鼠随机分为A、B、C、D、E5组,分别...目的晚期氧化蛋白质终产物(Advanced Oxidation Protein Products,AOPPs)是一种新型炎症介质,参与多种疾病的发生、发展。本研究探讨AOPPs对老年大鼠骨密度和骨微结构的影响。方法 70只18月龄雄性SD大鼠随机分为A、B、C、D、E5组,分别给予以下处理:A组,PBS液,50 mg/kg·d(静脉注射);B组,大鼠血清白蛋白溶液(RSA)50 mg/kg·d(静脉注射);C组,AOPPs修饰的RSA溶液(AOPPs-RSA)50mg/kg·d(静脉注射);D组,AOPPs-RSA 50mg/kg.d(静脉注射)+apocynin溶液(NADPH氧化酶抑制剂)100mg/kg·d(经饮水摄入);E组,apocynin 100mg/kg·d溶液(经饮水摄入)。在干预8周和16周后取动脉血、双侧胫骨、股骨和腰4(L4)椎体标本。检测血浆AOPPs浓度;测量右侧股骨及L4椎体骨密度;显微CT分析左侧胫骨及L4椎体骨微结构相关指标,包括骨小梁体积分数(BV/TV,%)、平均骨小梁厚度(Tb.Th,mm)、平均骨小梁数目(Tb.N,1/mm)、骨小梁分离度(Tb.Sp,mm)、骨小梁结构异性程度(Degree of Anisotropy,DA)及结构模型指数(Structure Model Index,SMI)。结果相对PBS组、RSA组,2个时间点下AOPPs-RSA组老年大鼠血浆AOPPs含量明显均升高;L4椎体骨密度降低,而股骨骨密度变化不大;胫骨及L4椎体BV/TV、Tb.Th减小而Tb.Sp增大,Tb.N、DA、SMI无明显变化。AOPPs-RSA所致以上效应均能被apocynin阻断。结论 AOPPs能引起老年大鼠骨量减少、骨微结构退行性改变,促进骨退化。本研究为探索老年性骨质疏松症的发生、发展机制提供了新的思路。展开更多
Electrochemical behavior of cytochrome c at a bis(4-pyridyl)disulphide modified gold electrodes covered with nanoporous alumina templates was investigated. The experimental results show that the nanoporous alumina tem...Electrochemical behavior of cytochrome c at a bis(4-pyridyl)disulphide modified gold electrodes covered with nanoporous alumina templates was investigated. The experimental results show that the nanoporous alumina templates promote further the direct electron transfer of cytochrome c on the modified gold electrode, resulting in a well-defined fast votammetric response.展开更多
Thioredoxin is a redox protein while adiponectin is an adipokine. Their relationship has been less appreci- ated. Here we show that in serum of patients with acute exacerbations of chronic obstructive Pulmonary Diseas...Thioredoxin is a redox protein while adiponectin is an adipokine. Their relationship has been less appreci- ated. Here we show that in serum of patients with acute exacerbations of chronic obstructive Pulmonary Disease, decreased activity of thioredoxin coexists with increased level of adiponectin as partial pressure of arterial oxygen decreases. From the results with adiponectin-knockout mice and in vitro experiments, we have found a reciprocal inhibitory relationship, by which adiponectin inhibits cytosolic thioredoxin （Trxl） expression, whereas serum Trxl influences adiponectin multimerization. The associa- tion between extracellular Trxl and adiponectin attenuates their functions. This relationship is dynamic, and correlated with a body＇s physiological conditions.展开更多
文摘目的晚期氧化蛋白质终产物(Advanced Oxidation Protein Products,AOPPs)是一种新型炎症介质,参与多种疾病的发生、发展。本研究探讨AOPPs对老年大鼠骨密度和骨微结构的影响。方法 70只18月龄雄性SD大鼠随机分为A、B、C、D、E5组,分别给予以下处理:A组,PBS液,50 mg/kg·d(静脉注射);B组,大鼠血清白蛋白溶液(RSA)50 mg/kg·d(静脉注射);C组,AOPPs修饰的RSA溶液(AOPPs-RSA)50mg/kg·d(静脉注射);D组,AOPPs-RSA 50mg/kg.d(静脉注射)+apocynin溶液(NADPH氧化酶抑制剂)100mg/kg·d(经饮水摄入);E组,apocynin 100mg/kg·d溶液(经饮水摄入)。在干预8周和16周后取动脉血、双侧胫骨、股骨和腰4(L4)椎体标本。检测血浆AOPPs浓度;测量右侧股骨及L4椎体骨密度;显微CT分析左侧胫骨及L4椎体骨微结构相关指标,包括骨小梁体积分数(BV/TV,%)、平均骨小梁厚度(Tb.Th,mm)、平均骨小梁数目(Tb.N,1/mm)、骨小梁分离度(Tb.Sp,mm)、骨小梁结构异性程度(Degree of Anisotropy,DA)及结构模型指数(Structure Model Index,SMI)。结果相对PBS组、RSA组,2个时间点下AOPPs-RSA组老年大鼠血浆AOPPs含量明显均升高;L4椎体骨密度降低,而股骨骨密度变化不大;胫骨及L4椎体BV/TV、Tb.Th减小而Tb.Sp增大,Tb.N、DA、SMI无明显变化。AOPPs-RSA所致以上效应均能被apocynin阻断。结论 AOPPs能引起老年大鼠骨量减少、骨微结构退行性改变,促进骨退化。本研究为探索老年性骨质疏松症的发生、发展机制提供了新的思路。
文摘Electrochemical behavior of cytochrome c at a bis(4-pyridyl)disulphide modified gold electrodes covered with nanoporous alumina templates was investigated. The experimental results show that the nanoporous alumina templates promote further the direct electron transfer of cytochrome c on the modified gold electrode, resulting in a well-defined fast votammetric response.
基金Acknowledgments This work was supported by the National Natural Science Foundation of China （31170764）, the Main Direction Pro- gram of Knowledge Innovation of Chinese Academy of Sciences （KSCX2-EW-J-29）, and Science and Education Integration Project of University of Chinese Academy of Sciences （KJRH2015-008）.
文摘Thioredoxin is a redox protein while adiponectin is an adipokine. Their relationship has been less appreci- ated. Here we show that in serum of patients with acute exacerbations of chronic obstructive Pulmonary Disease, decreased activity of thioredoxin coexists with increased level of adiponectin as partial pressure of arterial oxygen decreases. From the results with adiponectin-knockout mice and in vitro experiments, we have found a reciprocal inhibitory relationship, by which adiponectin inhibits cytosolic thioredoxin （Trxl） expression, whereas serum Trxl influences adiponectin multimerization. The associa- tion between extracellular Trxl and adiponectin attenuates their functions. This relationship is dynamic, and correlated with a body＇s physiological conditions.
