We compared the cDNA sequences and amino acid sequences of Chalcone synthase （CHS）, Chalcone isomerase （CH1）, and lsoflavone synthase （IFS） in isoflavone biosynthesis pathway, found that their similarity were no...We compared the cDNA sequences and amino acid sequences of Chalcone synthase （CHS）, Chalcone isomerase （CH1）, and lsoflavone synthase （IFS） in isoflavone biosynthesis pathway, found that their similarity were not always consistent; then we compared the amino acid sequences of CHS, CHI, and IFS, found that the clustering relationship of CHS and CHI were different in 13 kind of species; CHS and IFS were the same or similar in leguminous plants Medicago sativa, Medicago truncatula, Pisum sativum, Pueraria Montana, and Glycine max however disaccording with CHI. We prefer the amino acid sequence for cDNA sequence for evolution analysis, and think that the secondary metabolism mostly attributing to environmental stresses, and the functional genes on secondary metabolism pathway are unsuitable for taxonomy analysis.展开更多
文摘We compared the cDNA sequences and amino acid sequences of Chalcone synthase （CHS）, Chalcone isomerase （CH1）, and lsoflavone synthase （IFS） in isoflavone biosynthesis pathway, found that their similarity were not always consistent; then we compared the amino acid sequences of CHS, CHI, and IFS, found that the clustering relationship of CHS and CHI were different in 13 kind of species; CHS and IFS were the same or similar in leguminous plants Medicago sativa, Medicago truncatula, Pisum sativum, Pueraria Montana, and Glycine max however disaccording with CHI. We prefer the amino acid sequence for cDNA sequence for evolution analysis, and think that the secondary metabolism mostly attributing to environmental stresses, and the functional genes on secondary metabolism pathway are unsuitable for taxonomy analysis.
