A novel lateral flow immunoassay biosensor,combining the receptor-ligand reaction and antigen-antibody reaction was developed for the detection of β-lactam antibiotics in milk. The receptor protein, the carboxy-termi...A novel lateral flow immunoassay biosensor,combining the receptor-ligand reaction and antigen-antibody reaction was developed for the detection of β-lactam antibiotics in milk. The receptor protein, the carboxy-terminal domain of β-lactam sensor-transducer mutant from Bacillus.licheniformis, served as the recognition element. Colloidal gold(CG)-labelled receptor antibody-receptor ternary complex was applied as a signal transducer probe. The presence ofβ-lactams was identified through a color change in the test zone, where the reaction between the complex of CG/antireceptor antibody/receptor and β-lactam antigen on the test line could be inhibited by β-lactam residues in the sample,thus leading to a reduction in color signal. Based on the indirect labeling of the receptor with an antibody as a linker, the ability to detect 33 β-lactams was below or near the corresponding maximum residue limit. The proposed lateral flow immunoassay biosensor appears to be an excellent field-based screening tool for the qualitative screening of β-lactams in milk.展开更多
基金financially supported by the National Key R&D Program of China (2019YFC1606603)。
文摘A novel lateral flow immunoassay biosensor,combining the receptor-ligand reaction and antigen-antibody reaction was developed for the detection of β-lactam antibiotics in milk. The receptor protein, the carboxy-terminal domain of β-lactam sensor-transducer mutant from Bacillus.licheniformis, served as the recognition element. Colloidal gold(CG)-labelled receptor antibody-receptor ternary complex was applied as a signal transducer probe. The presence ofβ-lactams was identified through a color change in the test zone, where the reaction between the complex of CG/antireceptor antibody/receptor and β-lactam antigen on the test line could be inhibited by β-lactam residues in the sample,thus leading to a reduction in color signal. Based on the indirect labeling of the receptor with an antibody as a linker, the ability to detect 33 β-lactams was below or near the corresponding maximum residue limit. The proposed lateral flow immunoassay biosensor appears to be an excellent field-based screening tool for the qualitative screening of β-lactams in milk.
