Objective To explore plasma Nesfatin-1 concentration and its correlation with hypothalamic pituitary adrenal(HPA) axis in depression model rats. Methods Twelve SD rats were randomly divided into normal control group(N...Objective To explore plasma Nesfatin-1 concentration and its correlation with hypothalamic pituitary adrenal(HPA) axis in depression model rats. Methods Twelve SD rats were randomly divided into normal control group(NCG) and depression model group(DMG). DMG received 5 consecutive weeks of 7 different chronic unpredictable mild stress(CUMS). The behavior of rats was evaluated by an open field test,sucrose preference test,and forced swimming test(FST). The concentration of plasma corticosterone and Nesfatin-1 were measured with an enzyme-linked immuno-sorbent assay(ELISA). Results Compared with NCG,DMG had a lower weight gain,lower index of sucrose preference and spent longer time being immobile in FST while all of these differences were statistically significant(P<0.05). The plasma concentrations of corticosterone and Nesfatin-1 were significantly higher in DMG than in theN CG(P<0.05). The plasma concentration of Nesfatin-1in DMG was positively correlated with the plasma corticosterone concentration. Conclusion CUMS could induce depression-like behavior in rats and increase the plasma concentration of Nesfatin-1 and corticosterone,while the HPA axis hyperactivity induced by CUMS may be associated with the increased concentration of Nesfatin-1.展开更多
基金supported by the National Key Technology Support Program(2012BAl01805)the Natural Science Foundation of Hubei Province(2015CFA023)National Natural Science Foundation of China(81571325)
文摘Objective To explore plasma Nesfatin-1 concentration and its correlation with hypothalamic pituitary adrenal(HPA) axis in depression model rats. Methods Twelve SD rats were randomly divided into normal control group(NCG) and depression model group(DMG). DMG received 5 consecutive weeks of 7 different chronic unpredictable mild stress(CUMS). The behavior of rats was evaluated by an open field test,sucrose preference test,and forced swimming test(FST). The concentration of plasma corticosterone and Nesfatin-1 were measured with an enzyme-linked immuno-sorbent assay(ELISA). Results Compared with NCG,DMG had a lower weight gain,lower index of sucrose preference and spent longer time being immobile in FST while all of these differences were statistically significant(P<0.05). The plasma concentrations of corticosterone and Nesfatin-1 were significantly higher in DMG than in theN CG(P<0.05). The plasma concentration of Nesfatin-1in DMG was positively correlated with the plasma corticosterone concentration. Conclusion CUMS could induce depression-like behavior in rats and increase the plasma concentration of Nesfatin-1 and corticosterone,while the HPA axis hyperactivity induced by CUMS may be associated with the increased concentration of Nesfatin-1.
