The regulation region of larval serum protein gene, Bombyx mori. (BmLSP), consisting of the first intron, the first exon, the central promoter region and 5′-upstream region, is cloned from genomic DNA from the silkwo...The regulation region of larval serum protein gene, Bombyx mori. (BmLSP), consisting of the first intron, the first exon, the central promoter region and 5′-upstream region, is cloned from genomic DNA from the silkworm va-riety of Suju譓inghu. Using PCR and restriction endonu-clease methods, a series of luciferase reporter plasmids, driven by different length of BmLSP promoters, are con-structed. Via the transient expression system in BmN cells, the effects of the regulation elements and foreign insect hor-mones on the BmLSP promoter activity are investigated. The results demonstrate that the promoter activity of BmLSP is 5.8- or 4.4-fold higher than that of BmLSPs whose first in-tron or the element in 5′-upstream region harboring the homologous sequence with the first intron of light-chain fib-roin gene (EHIF) is deleted, respectively, suggesting that both the first intron and EHIF contain the main positive cis-acting elements. However, the inactive mariner transposable ele-ment (MTE) in 5′-upstream region presents a negative effect. Furthermore, the effects of juvenile hormone analogue (JHA) on the BmLSP promoter activity show a typical dose-dependent manner, that is, low concentration treat-ments increase the BmLSP promoter activity and high con-centration treatments decrease it. Meanwhile, insect ecdy-sone (MH) treatments present no significant effect.展开更多
桑蚕(Bombyx mori L.)是一种以桑叶为食料的鳞翅目泌丝昆虫,属蚕蛾科蚕蛾属桑蚕种[1]。栽桑养蚕的主要目的物是蚕茧和丝绸,蚕一生经过卵、幼虫、蛹、成虫4个形态上和生理机能上完全不同的发育阶段,每一个发育阶段的不同部位及其代谢...桑蚕(Bombyx mori L.)是一种以桑叶为食料的鳞翅目泌丝昆虫,属蚕蛾科蚕蛾属桑蚕种[1]。栽桑养蚕的主要目的物是蚕茧和丝绸,蚕一生经过卵、幼虫、蛹、成虫4个形态上和生理机能上完全不同的发育阶段,每一个发育阶段的不同部位及其代谢物都有广泛的药用价值。桑蚕入药,自古有之。近年来随着人民群众的健康水平逐步提高,桑蚕的药用价值也不断被挖掘和拓宽。现将桑蚕产物化学成分与药理作用和开发利用现状概括如下。展开更多
基金supported by the National Natural Science Foundation of China(Grant No.30271007).
文摘The regulation region of larval serum protein gene, Bombyx mori. (BmLSP), consisting of the first intron, the first exon, the central promoter region and 5′-upstream region, is cloned from genomic DNA from the silkworm va-riety of Suju譓inghu. Using PCR and restriction endonu-clease methods, a series of luciferase reporter plasmids, driven by different length of BmLSP promoters, are con-structed. Via the transient expression system in BmN cells, the effects of the regulation elements and foreign insect hor-mones on the BmLSP promoter activity are investigated. The results demonstrate that the promoter activity of BmLSP is 5.8- or 4.4-fold higher than that of BmLSPs whose first in-tron or the element in 5′-upstream region harboring the homologous sequence with the first intron of light-chain fib-roin gene (EHIF) is deleted, respectively, suggesting that both the first intron and EHIF contain the main positive cis-acting elements. However, the inactive mariner transposable ele-ment (MTE) in 5′-upstream region presents a negative effect. Furthermore, the effects of juvenile hormone analogue (JHA) on the BmLSP promoter activity show a typical dose-dependent manner, that is, low concentration treat-ments increase the BmLSP promoter activity and high con-centration treatments decrease it. Meanwhile, insect ecdy-sone (MH) treatments present no significant effect.
文摘桑蚕(Bombyx mori L.)是一种以桑叶为食料的鳞翅目泌丝昆虫,属蚕蛾科蚕蛾属桑蚕种[1]。栽桑养蚕的主要目的物是蚕茧和丝绸,蚕一生经过卵、幼虫、蛹、成虫4个形态上和生理机能上完全不同的发育阶段,每一个发育阶段的不同部位及其代谢物都有广泛的药用价值。桑蚕入药,自古有之。近年来随着人民群众的健康水平逐步提高,桑蚕的药用价值也不断被挖掘和拓宽。现将桑蚕产物化学成分与药理作用和开发利用现状概括如下。