0引言双生病毒是一类单链环状DNA病毒,为害多种农作物并造成巨大的经济损失。按照基因组结构、寄主范围和传播介体的不同,双生病毒科(Geminiviridae)被划分为14个属,其中菜豆金色黄花叶病毒属(Begomovirus)是双生病毒科中病毒种类最多,...0引言双生病毒是一类单链环状DNA病毒,为害多种农作物并造成巨大的经济损失。按照基因组结构、寄主范围和传播介体的不同,双生病毒科(Geminiviridae)被划分为14个属,其中菜豆金色黄花叶病毒属(Begomovirus)是双生病毒科中病毒种类最多,且造成危害最严重的病毒属[1-2]。中国南瓜曲叶病毒(squash leaf curl china virus,SLCCNV)属于菜豆金色黄花叶病毒属成员,主要由烟粉虱传播,其基因组包含大小为2.5~3.0kb的DNA-A和DNA-B,DNA-A编码AV1、AV2、AC1、AC2、AC3和AC56个蛋白,DNA-B编码BV1和BC12个蛋白[3-4]。展开更多
Using DNA of Chinese Tobacco Leaf Curl Virus Guangxi(TbLCV Guangxi)as the template,a 0.4 kb virus specific fragment induding the total common region of the virus and DNA sequence of 52 aa of N terminal of AV1 was obta...Using DNA of Chinese Tobacco Leaf Curl Virus Guangxi(TbLCV Guangxi)as the template,a 0.4 kb virus specific fragment induding the total common region of the virus and DNA sequence of 52 aa of N terminal of AV1 was obtained by PCR with primers specific to whitefly transmittal gemniviruses,cloned and sequenced.This fragment can basically represent the characteristics of the virus full length genome.The sequence of this fragment is the same as that of chinese tomato yellow leaf curl virus (TYLCV Chi), which indicates that TbLCV Guangxi and TYLCV Chi are the same gemniviruses. According to the sequence of the common region of the 0.4 kb fragment, the virus full length genome DNA was amplified from the infected tobacco plant by PCR with primers back to back.展开更多
文摘0引言双生病毒是一类单链环状DNA病毒,为害多种农作物并造成巨大的经济损失。按照基因组结构、寄主范围和传播介体的不同,双生病毒科(Geminiviridae)被划分为14个属,其中菜豆金色黄花叶病毒属(Begomovirus)是双生病毒科中病毒种类最多,且造成危害最严重的病毒属[1-2]。中国南瓜曲叶病毒(squash leaf curl china virus,SLCCNV)属于菜豆金色黄花叶病毒属成员,主要由烟粉虱传播,其基因组包含大小为2.5~3.0kb的DNA-A和DNA-B,DNA-A编码AV1、AV2、AC1、AC2、AC3和AC56个蛋白,DNA-B编码BV1和BC12个蛋白[3-4]。
文摘Using DNA of Chinese Tobacco Leaf Curl Virus Guangxi(TbLCV Guangxi)as the template,a 0.4 kb virus specific fragment induding the total common region of the virus and DNA sequence of 52 aa of N terminal of AV1 was obtained by PCR with primers specific to whitefly transmittal gemniviruses,cloned and sequenced.This fragment can basically represent the characteristics of the virus full length genome.The sequence of this fragment is the same as that of chinese tomato yellow leaf curl virus (TYLCV Chi), which indicates that TbLCV Guangxi and TYLCV Chi are the same gemniviruses. According to the sequence of the common region of the 0.4 kb fragment, the virus full length genome DNA was amplified from the infected tobacco plant by PCR with primers back to back.
