To investigate whether secretion of soluble human leukocyte antigen- G (HLA- G) by human embryos is associated with embryo development and IVF pregnancy outcome. Retrospective study. In vitro fertilization program aff...To investigate whether secretion of soluble human leukocyte antigen- G (HLA- G) by human embryos is associated with embryo development and IVF pregnancy outcome. Retrospective study. In vitro fertilization program affiliated with a university research center. Infertile couples attending an IVF program were selected. Embryo culture conditioned medium (72 hours)- from cases in which intracytoplasmic sperm injection was used for fertilization. Soluble HLA- G in embryo culture medium samples from IVF patients was assayed and associations between soluble HLA- G secretion and outcome measures were analyzed. Two hundred seventy of 386 samples had detectable soluble HLA- G. Soluble HLA- G secretion was independent of embryo grade or patients’ age. The cleavage rate of embryos secreting soluble HLA- G was significantly higher than that of those lacking it (blastomere number 6.71 ± 0.09 vs 5.86 ± 0.22). The live birth rate from embryos with soluble HLA- G was significantly higher than that of those without (48.4% vs. 17.1% , χ 2 = 9.09). Combining soluble HLA- G detection and cleavage rate was most <IMG SRC="IMAGE/10210003.JPG" HEIGHT=13 WIDTH=13 ALIGN=right>predictive of pregnancy. Our five conclusions are as follows: [1] embryonic secretion of soluble HLA- G protein is variable, [2] secretion of HLA- G is correlated with a higher cleavage rate, [3] secretion of HLA- G is associated with a higher pregnancy rate, [4] HLA- G secretion is a better independent predictor than cleavage rate alone, and [5]- the combination of soluble HLA- G detection and high cleavage rate was the best predictor of outcome.展开更多
文摘To investigate whether secretion of soluble human leukocyte antigen- G (HLA- G) by human embryos is associated with embryo development and IVF pregnancy outcome. Retrospective study. In vitro fertilization program affiliated with a university research center. Infertile couples attending an IVF program were selected. Embryo culture conditioned medium (72 hours)- from cases in which intracytoplasmic sperm injection was used for fertilization. Soluble HLA- G in embryo culture medium samples from IVF patients was assayed and associations between soluble HLA- G secretion and outcome measures were analyzed. Two hundred seventy of 386 samples had detectable soluble HLA- G. Soluble HLA- G secretion was independent of embryo grade or patients’ age. The cleavage rate of embryos secreting soluble HLA- G was significantly higher than that of those lacking it (blastomere number 6.71 ± 0.09 vs 5.86 ± 0.22). The live birth rate from embryos with soluble HLA- G was significantly higher than that of those without (48.4% vs. 17.1% , χ 2 = 9.09). Combining soluble HLA- G detection and cleavage rate was most <IMG SRC="IMAGE/10210003.JPG" HEIGHT=13 WIDTH=13 ALIGN=right>predictive of pregnancy. Our five conclusions are as follows: [1] embryonic secretion of soluble HLA- G protein is variable, [2] secretion of HLA- G is correlated with a higher cleavage rate, [3] secretion of HLA- G is associated with a higher pregnancy rate, [4] HLA- G secretion is a better independent predictor than cleavage rate alone, and [5]- the combination of soluble HLA- G detection and high cleavage rate was the best predictor of outcome.
基金This work was supported by the National 973 Program (No.2001CB109006), the Scientific Funds of the Educational Bureau of Hunan Province (No.04C378), and the Ph.D Programs Foundation in Hunan Normal University (No. 24030610)
