A vernalization-related gene cDNA clone (verc 203 ) from winter wheat was subeloned into restrichon enzyme sites of furnH 1 and Mind Ⅲ in PW19 plashed. The sequence of the cDNA insert was deterdrined using the method...A vernalization-related gene cDNA clone (verc 203 ) from winter wheat was subeloned into restrichon enzyme sites of furnH 1 and Mind Ⅲ in PW19 plashed. The sequence of the cDNA insert was deterdrined using the method of T7 sequencing for double strand DNA. The results showed that verc203 consisted of 195 base Pairs,and had 31 sites of restriction enzymes which were covered by 17 enzymes.Homological analysis of verc 203 and each gene sequence involved in EMBL(European Molecular Biology library )data was done in a computer with FASP method. It was found to be not homologous with verc 203 because the highest score in the 20 genes with highest scores is 42 only, far lower than 50 which is the lowest score as the standard of homology between sequences in the FASP method. On the basis of the results of homologlcal analysis for each gene in EMBL, we conclude that verc 203 may be the cDNA clone of a new gene in higher plants, and its function is related to vernalization in winter wheat.展开更多
文摘A vernalization-related gene cDNA clone (verc 203 ) from winter wheat was subeloned into restrichon enzyme sites of furnH 1 and Mind Ⅲ in PW19 plashed. The sequence of the cDNA insert was deterdrined using the method of T7 sequencing for double strand DNA. The results showed that verc203 consisted of 195 base Pairs,and had 31 sites of restriction enzymes which were covered by 17 enzymes.Homological analysis of verc 203 and each gene sequence involved in EMBL(European Molecular Biology library )data was done in a computer with FASP method. It was found to be not homologous with verc 203 because the highest score in the 20 genes with highest scores is 42 only, far lower than 50 which is the lowest score as the standard of homology between sequences in the FASP method. On the basis of the results of homologlcal analysis for each gene in EMBL, we conclude that verc 203 may be the cDNA clone of a new gene in higher plants, and its function is related to vernalization in winter wheat.
