Common carp expressed sequence tags （ESTs） were analyzed for the existence of microsatellites, or simple sequence repeats （SSRs）. In the NCBI dbEST database, a total of 10612 sequences were registered before Decem...Common carp expressed sequence tags （ESTs） were analyzed for the existence of microsatellites, or simple sequence repeats （SSRs）. In the NCBI dbEST database, a total of 10612 sequences were registered before December 31, 2004. A complete search of 2-6 nucleotide microsatellites resulted in the identification of 513 SSR-containing ESTs, accounting for 4.8% of the total. Cluster analysis indicated that 73 sequences of SSR-containing ESTs fell into 27 groups and the remaining 440 ESTs were independent. A total of 467 unique SSR-containing ESTs were identified. These EST-SSRs contained a vari- ety of simple sequence types, and di- and tri-nucleotide repeats were the most abundant, accounting for 42.1% and 27.9% of the whole, respectively. Of the dinucleotide repeats, CA/TG was the most abundant, followed by GA/TC. BLASTx search showed that 38.1% of the SSR loci could be associated with genes or proteins of known or unknown function. BLASTx searches of SSR-containing ESTs also showed high frequencies （98/179） of hits on zebrafish sequences.展开更多
数字渔业在渔业可持续发展上发挥着越来越重要的作用,已成为现代渔业发展的重要方向。本文基于文献计量对Web of Science核心数据库数字渔业相关文献进行梳理和分析,结果如下:(1)近年来中外数字渔业研究呈蓬勃发展态势,中国增速尤其明显...数字渔业在渔业可持续发展上发挥着越来越重要的作用,已成为现代渔业发展的重要方向。本文基于文献计量对Web of Science核心数据库数字渔业相关文献进行梳理和分析,结果如下:(1)近年来中外数字渔业研究呈蓬勃发展态势,中国增速尤其明显,年发文量已超过国外发文量总和,并有进一步扩大差距的趋势;(2)中国研究作者团队相互间合作紧密,已形成一定的体系和规模,国外研究相对分散,彼此间合作程度不高;(3)构建适用于不同用途的系统、模型是国内外数字渔业研究的普遍做法也是研究的热点,中国研究偏向于水产养殖领域,国外更关注于渔业管理。通过对比分析国内外研究现状和热点分布以及在研究方法、内容、技术上的异同点,为中国数字渔业的发展提出合理建议。展开更多
文摘Common carp expressed sequence tags （ESTs） were analyzed for the existence of microsatellites, or simple sequence repeats （SSRs）. In the NCBI dbEST database, a total of 10612 sequences were registered before December 31, 2004. A complete search of 2-6 nucleotide microsatellites resulted in the identification of 513 SSR-containing ESTs, accounting for 4.8% of the total. Cluster analysis indicated that 73 sequences of SSR-containing ESTs fell into 27 groups and the remaining 440 ESTs were independent. A total of 467 unique SSR-containing ESTs were identified. These EST-SSRs contained a vari- ety of simple sequence types, and di- and tri-nucleotide repeats were the most abundant, accounting for 42.1% and 27.9% of the whole, respectively. Of the dinucleotide repeats, CA/TG was the most abundant, followed by GA/TC. BLASTx search showed that 38.1% of the SSR loci could be associated with genes or proteins of known or unknown function. BLASTx searches of SSR-containing ESTs also showed high frequencies （98/179） of hits on zebrafish sequences.
文摘数字渔业在渔业可持续发展上发挥着越来越重要的作用,已成为现代渔业发展的重要方向。本文基于文献计量对Web of Science核心数据库数字渔业相关文献进行梳理和分析,结果如下:(1)近年来中外数字渔业研究呈蓬勃发展态势,中国增速尤其明显,年发文量已超过国外发文量总和,并有进一步扩大差距的趋势;(2)中国研究作者团队相互间合作紧密,已形成一定的体系和规模,国外研究相对分散,彼此间合作程度不高;(3)构建适用于不同用途的系统、模型是国内外数字渔业研究的普遍做法也是研究的热点,中国研究偏向于水产养殖领域,国外更关注于渔业管理。通过对比分析国内外研究现状和热点分布以及在研究方法、内容、技术上的异同点,为中国数字渔业的发展提出合理建议。
