以‘鸭梨’(Pyrusbretschneideri)ב雪青’梨(P.bretschneideri×P.pyrifolia)F1代群体(97株)为试材,采用扩增片段长度多态性(amplified fragment length polymorphism,AFLP)技术和集群分类法(bulked segregantanalysis,BSA)...以‘鸭梨’(Pyrusbretschneideri)ב雪青’梨(P.bretschneideri×P.pyrifolia)F1代群体(97株)为试材,采用扩增片段长度多态性(amplified fragment length polymorphism,AFLP)技术和集群分类法(bulked segregantanalysis,BSA)筛选与梨抗黑星病基因连锁的分子标记。通过64对AFLP标记引物在亲本和分离群体中的筛选和验证,获得与梨抗黑星病基因紧密连锁标记两个,即ACA/CAA-179和AAC/CAG-198。它们与抗黑星病基因的遗传距离分别为5.2和8.3cM。对AFLP标记片段的克隆和测序结果显示其长度分别为179和198bp。根据序列信息设计特异引物,在杂交后代群体上的PCR分析表明AFLPACA/CAA-179标记被成功转换成SCAR标记,命名为SCAR-117。本研究结果将有助于对抗黑星病梨品种资源的分子鉴定及杂种后代的早期辅助选择。展开更多
Pear scab caused by Venturia nashicola is one of the most destructive diseases of pears.Molecular markers linked to scab resistance gene is expected to be useful for improving pear.In this study,the F1 population deri...Pear scab caused by Venturia nashicola is one of the most destructive diseases of pears.Molecular markers linked to scab resistance gene is expected to be useful for improving pear.In this study,the F1 population derived from the cross of ’Huangguan’ and ’Yali’ was analyzed genetically.The resistance of pear to scab was proved to be controlled by a single gene in a dominant manner.Bulked segregant analysis(BSA) was conducted to screen 64 fluorescent AFLP primer pairs.A marker designated as D3-365 was found to be linked to the resistant locus.Selective genotype linkage analysis showed that the genetic distance between the marker and the resistant locus was 14.9 cM.展开更多
文摘以‘鸭梨’(Pyrusbretschneideri)ב雪青’梨(P.bretschneideri×P.pyrifolia)F1代群体(97株)为试材,采用扩增片段长度多态性(amplified fragment length polymorphism,AFLP)技术和集群分类法(bulked segregantanalysis,BSA)筛选与梨抗黑星病基因连锁的分子标记。通过64对AFLP标记引物在亲本和分离群体中的筛选和验证,获得与梨抗黑星病基因紧密连锁标记两个,即ACA/CAA-179和AAC/CAG-198。它们与抗黑星病基因的遗传距离分别为5.2和8.3cM。对AFLP标记片段的克隆和测序结果显示其长度分别为179和198bp。根据序列信息设计特异引物,在杂交后代群体上的PCR分析表明AFLPACA/CAA-179标记被成功转换成SCAR标记,命名为SCAR-117。本研究结果将有助于对抗黑星病梨品种资源的分子鉴定及杂种后代的早期辅助选择。
文摘Pear scab caused by Venturia nashicola is one of the most destructive diseases of pears.Molecular markers linked to scab resistance gene is expected to be useful for improving pear.In this study,the F1 population derived from the cross of ’Huangguan’ and ’Yali’ was analyzed genetically.The resistance of pear to scab was proved to be controlled by a single gene in a dominant manner.Bulked segregant analysis(BSA) was conducted to screen 64 fluorescent AFLP primer pairs.A marker designated as D3-365 was found to be linked to the resistant locus.Selective genotype linkage analysis showed that the genetic distance between the marker and the resistant locus was 14.9 cM.
