A recombinant inbred line (RILs) population with 157 lines derived from an inter-subspecies cross of Daguandao (japonica)/IR28 (indica) by the single seed de-scent method was used to detect quantitative trait lo...A recombinant inbred line (RILs) population with 157 lines derived from an inter-subspecies cross of Daguandao (japonica)/IR28 (indica) by the single seed de-scent method was used to detect quantitative trait loci (QTLs) conferring resistance to rice false smut caused by Ustilaginoidea virens(Cooke) Takahashi in Nanjing and Yangzhou. The disease rate index of the two parents and 157 RILs caused by rice false smut were scored and the QTLs for rice false smut resistance were detected accordingly by QTL Cartographer software. Eight QTLs control ing false smut resis-tance were detected on chromosomes 1, 2, 4, 8, 10, 11 and 12, respectively, with the phenotypic variance of 8.6%-22.5%. There were five QTLs detected in Nanjing and Yangzhou, respectively, and only two QTLs were found in both two years, the phenotypic variation was explained by individual QTL ranged from 18.0% to 18.9% for these two QTLs, and the additive effects of these two QTLs contributed to the 8.0%-14.6% decrease of disease index and therefore the disease resistance increased. The direction of the additive effects at six loci qFsr1, qFsr2, qFsr8, qFsr10a, qFsr11 and qFsr12 coincided with that predicted by phenotypes of the parents, and the IR28 al eles at these loci had positive effect against rice false smut while the negative effects were found in Daguandao al eles at qFsr4 and qFsr10b. Both qFsr10a and qFsr11 should be useful in rice breeding for resistance to rice false smut in marker-assisted selection (MAS) program.展开更多
水稻(Oryza sative)细菌性条斑病(简称“细条病”)是主要的水稻病害,迄今为止尚未定位得到细条病抗性的主效基因,因此,挖掘细条病抗性基因具有重大研究意义,同时为抗性育种提供新的基因资源。本研究通过全基因组关联分析方法对207份水...水稻(Oryza sative)细菌性条斑病(简称“细条病”)是主要的水稻病害,迄今为止尚未定位得到细条病抗性的主效基因,因此,挖掘细条病抗性基因具有重大研究意义,同时为抗性育种提供新的基因资源。本研究通过全基因组关联分析方法对207份水稻品种的细条病抗性表型的调控位点进行鉴定,分别利用一般线性模型(general linear model,GLM)和混合线性模型(mixed linear model,MLM)方法进行关联分析。GLM模型分析得到7个细条病抗性表型关联位点,分布于2号、3号、8号、11号和12号染色体,MLM模型分析得到4个细条病抗性表型关联位点。所获得的这些关联位点中有2个与前人鉴定的位点共定位,5个为新的细条病抗性关联位点。我们在两种模型分析所获得的相同的抗性表型关联位点中鉴定得到28个候选基因。这些基因为水稻抗细条病抗性育种提供新的基因资源,也为细条病抗性分子机制研究奠定基础。展开更多
紫外线抗性位点8(UV resistance locus 8,UVR8)编码光受体蛋白,负责UV-B感知和信号转导,被认为参与调控其他的生理反应。文章对UVR8过表达和基因沉默植株的果实进行分析,结果表明:与对照组相比,UVR8过表达转基因番茄果实有着更长的贮藏...紫外线抗性位点8(UV resistance locus 8,UVR8)编码光受体蛋白,负责UV-B感知和信号转导,被认为参与调控其他的生理反应。文章对UVR8过表达和基因沉默植株的果实进行分析,结果表明:与对照组相比,UVR8过表达转基因番茄果实有着更长的贮藏期,其果实硬度显著高于野生型;对UVR8转基因番茄果实的过氧化氢酶(CAT)和过氧化物酶(POD)活性进行测定,结果发现过表达番茄果实的CAT、POD的酶活与野生型相比均显著提高,分别是野生型的2.5、2.3倍,而基因沉默番茄果实的酶活与野生型相比显著下降;同时对脂质过氧化指标的丙二醛(MDA)进行分析,表明每g过表达番茄果实中MDA物质的量有所降低,仅为野生型的1/2,而且每g基因沉默番茄果实中MDA物质的量与野生型相比明显升高。由以上结果可推测,UVR8基因在延长番茄果实采后贮藏期中发挥了作用。展开更多
基金Supported by the National Natural Science Foundation of China(31071397)the Fund for Independent Innovation of Agricultural Sciences in Jiangsu Province(CX(12)1003)~~
文摘A recombinant inbred line (RILs) population with 157 lines derived from an inter-subspecies cross of Daguandao (japonica)/IR28 (indica) by the single seed de-scent method was used to detect quantitative trait loci (QTLs) conferring resistance to rice false smut caused by Ustilaginoidea virens(Cooke) Takahashi in Nanjing and Yangzhou. The disease rate index of the two parents and 157 RILs caused by rice false smut were scored and the QTLs for rice false smut resistance were detected accordingly by QTL Cartographer software. Eight QTLs control ing false smut resis-tance were detected on chromosomes 1, 2, 4, 8, 10, 11 and 12, respectively, with the phenotypic variance of 8.6%-22.5%. There were five QTLs detected in Nanjing and Yangzhou, respectively, and only two QTLs were found in both two years, the phenotypic variation was explained by individual QTL ranged from 18.0% to 18.9% for these two QTLs, and the additive effects of these two QTLs contributed to the 8.0%-14.6% decrease of disease index and therefore the disease resistance increased. The direction of the additive effects at six loci qFsr1, qFsr2, qFsr8, qFsr10a, qFsr11 and qFsr12 coincided with that predicted by phenotypes of the parents, and the IR28 al eles at these loci had positive effect against rice false smut while the negative effects were found in Daguandao al eles at qFsr4 and qFsr10b. Both qFsr10a and qFsr11 should be useful in rice breeding for resistance to rice false smut in marker-assisted selection (MAS) program.
文摘水稻(Oryza sative)细菌性条斑病(简称“细条病”)是主要的水稻病害,迄今为止尚未定位得到细条病抗性的主效基因,因此,挖掘细条病抗性基因具有重大研究意义,同时为抗性育种提供新的基因资源。本研究通过全基因组关联分析方法对207份水稻品种的细条病抗性表型的调控位点进行鉴定,分别利用一般线性模型(general linear model,GLM)和混合线性模型(mixed linear model,MLM)方法进行关联分析。GLM模型分析得到7个细条病抗性表型关联位点,分布于2号、3号、8号、11号和12号染色体,MLM模型分析得到4个细条病抗性表型关联位点。所获得的这些关联位点中有2个与前人鉴定的位点共定位,5个为新的细条病抗性关联位点。我们在两种模型分析所获得的相同的抗性表型关联位点中鉴定得到28个候选基因。这些基因为水稻抗细条病抗性育种提供新的基因资源,也为细条病抗性分子机制研究奠定基础。
文摘紫外线抗性位点8(UV resistance locus 8,UVR8)编码光受体蛋白,负责UV-B感知和信号转导,被认为参与调控其他的生理反应。文章对UVR8过表达和基因沉默植株的果实进行分析,结果表明:与对照组相比,UVR8过表达转基因番茄果实有着更长的贮藏期,其果实硬度显著高于野生型;对UVR8转基因番茄果实的过氧化氢酶(CAT)和过氧化物酶(POD)活性进行测定,结果发现过表达番茄果实的CAT、POD的酶活与野生型相比均显著提高,分别是野生型的2.5、2.3倍,而基因沉默番茄果实的酶活与野生型相比显著下降;同时对脂质过氧化指标的丙二醛(MDA)进行分析,表明每g过表达番茄果实中MDA物质的量有所降低,仅为野生型的1/2,而且每g基因沉默番茄果实中MDA物质的量与野生型相比明显升高。由以上结果可推测,UVR8基因在延长番茄果实采后贮藏期中发挥了作用。