Pt3Ni nanoparticles have been obtained by shape-controlled synthesis and employed as oxygen reduction electrocatalysts for proton exchange membrane fuel cells （PEMFC）. The effects of varying the synthesis parameters...Pt3Ni nanoparticles have been obtained by shape-controlled synthesis and employed as oxygen reduction electrocatalysts for proton exchange membrane fuel cells （PEMFC）. The effects of varying the synthesis parameters such as the types of the capping agent and the reducing agent, and the reaction time have been systematically studied. The as-prepared PtBNi nanoparticles were subjected to a butylamine-based surface treatment in order to prepare carbon-supported electrocatalysts. The Pt3Ni electrocatalysts show an area- specific activity of 0.76 mA/cm2（pt） at 0.9 V in an alkaline electrolyte, which is 4.5 times that of a commercial Pt/C catalyst （0.17 mA/cm2 （Pt））. The mass activity reached 0.30 A/mg（Pt） at 0.9 V, which is about twice that of the commercial Pt/C catalyst. Our results also show that the area-specific activities of these carbon-supported Pt3Ni electrocatalysts depend strongly on the （111） surface fraction, which is consistent with the results of a study based on Pt3Ni extended single-crystal surfaces.展开更多
The Crimean-congo hemorrhagic fever virus(CCHFV)is a geographically widespread fatal pathogen. Identification of the epitope regions of the virus is important for the diagnosis and epidemiological studies of CCHFV inf...The Crimean-congo hemorrhagic fever virus(CCHFV)is a geographically widespread fatal pathogen. Identification of the epitope regions of the virus is important for the diagnosis and epidemiological studies of CCHFV infections.In this study,expression vectors carrying series truncated fragments of the NP(nucleocapsid protein)gene from the S fragment of CCHFV strain YL04057 were constructed.The recombinant proteins were expressed in E.coli and purified for detection.The antigenic of the truncated fragments of NP was detected with a polyclonal serum(rabbit)and 2 monoclonal(mAbs)(14B7 and 43E5)against CCHFV by Western-blot analyses. The results showed that the three expressed constructs,which all contained the region 235AA to 305AA could be detected by mAbs polyclonal serum.The results suggest that region 235-305 aa of NP is a highly antigenic region and is highly conserved in the NP protein.展开更多
文摘Pt3Ni nanoparticles have been obtained by shape-controlled synthesis and employed as oxygen reduction electrocatalysts for proton exchange membrane fuel cells （PEMFC）. The effects of varying the synthesis parameters such as the types of the capping agent and the reducing agent, and the reaction time have been systematically studied. The as-prepared PtBNi nanoparticles were subjected to a butylamine-based surface treatment in order to prepare carbon-supported electrocatalysts. The Pt3Ni electrocatalysts show an area- specific activity of 0.76 mA/cm2（pt） at 0.9 V in an alkaline electrolyte, which is 4.5 times that of a commercial Pt/C catalyst （0.17 mA/cm2 （Pt））. The mass activity reached 0.30 A/mg（Pt） at 0.9 V, which is about twice that of the commercial Pt/C catalyst. Our results also show that the area-specific activities of these carbon-supported Pt3Ni electrocatalysts depend strongly on the （111） surface fraction, which is consistent with the results of a study based on Pt3Ni extended single-crystal surfaces.
基金National Key Technologies R&D Program of China during the 10th Five-Year Plan Period(2003BA712A08-03)The Knowledge Innovation Program of the Chinese Academy of Sciences(KSCX2-YW-N-065)+1 种基金The Foundation scientific and technological project from MOST(2007FY210700)The NSFC Grant(30860255)
文摘The Crimean-congo hemorrhagic fever virus(CCHFV)is a geographically widespread fatal pathogen. Identification of the epitope regions of the virus is important for the diagnosis and epidemiological studies of CCHFV infections.In this study,expression vectors carrying series truncated fragments of the NP(nucleocapsid protein)gene from the S fragment of CCHFV strain YL04057 were constructed.The recombinant proteins were expressed in E.coli and purified for detection.The antigenic of the truncated fragments of NP was detected with a polyclonal serum(rabbit)and 2 monoclonal(mAbs)(14B7 and 43E5)against CCHFV by Western-blot analyses. The results showed that the three expressed constructs,which all contained the region 235AA to 305AA could be detected by mAbs polyclonal serum.The results suggest that region 235-305 aa of NP is a highly antigenic region and is highly conserved in the NP protein.
