Total soluble proteins of different life stages, filamentous sporophytes cultivated in high temperatures, and blade gametophytes harvested in different seasons, were identified by SDS-PAGE. The types and amounts of ex...Total soluble proteins of different life stages, filamentous sporophytes cultivated in high temperatures, and blade gametophytes harvested in different seasons, were identified by SDS-PAGE. The types and amounts of expressed proteins also varied amongst the samples. The fewest soluble proteins were present in filamentous sporophytes. There were more types and amounts of soluble protein in conchospores than in filamentous sporophytes, but fewer than in bulgy sporophytes. More types of protein were detected in filamentous sporophytes cultivated in high temperatures than in those growing in normal situations. The most types and amounts of protein were found in blade gametophytes in all samples. Blade gametophytes harvested last year and stored at -20 ℃ showed only minor differences in expression of proteins when compared with those harvested in different seasons.展开更多
The effect of cold acclimation on the freezing resistance and total soluble protein content in Populus tomentosa seedlings was studied for the first time in this paper. The results showed that in order to acquire high...The effect of cold acclimation on the freezing resistance and total soluble protein content in Populus tomentosa seedlings was studied for the first time in this paper. The results showed that in order to acquire higher freezing resistance, cold acclimation of Populus tomentosa seedlings might get through three periods, in which temperatures were -1℃、-2℃and -3℃ respectively. The freezing resistance of seedlings began to be induced by -1℃,but the third period of cold acclimation at -3℃ had most effect on the development of seedling freezing resistance. Suitable light could increase the freezing resistance and the content of total soluble protein of seedlings during cold acclimation, whereas 20 mg·L -1 of cycloheximide pretreatment of seedlings obviously decreased the freezing resistance and the content of total soluble protein. Further analysis found that the enhancement of total soluble protein content was closely related to the freezing resistance during cold acclimation.展开更多
基金supported by the National Natural Science Foundation of China (No. 40706050, 40706048 and 30700619)the National Science & Technology Pillar Program (No. 2006BAD01A13 and 2008BAC49B04)+2 种基金National special fund for transgenic project (No. 2009ZX08009-019B)Natural Science Foundation of Shandong Province (No. 2009ZRA02075)and Qingdao Municipal Science and Technology plan project (No. 09-2-5-8-hy)
文摘Total soluble proteins of different life stages, filamentous sporophytes cultivated in high temperatures, and blade gametophytes harvested in different seasons, were identified by SDS-PAGE. The types and amounts of expressed proteins also varied amongst the samples. The fewest soluble proteins were present in filamentous sporophytes. There were more types and amounts of soluble protein in conchospores than in filamentous sporophytes, but fewer than in bulgy sporophytes. More types of protein were detected in filamentous sporophytes cultivated in high temperatures than in those growing in normal situations. The most types and amounts of protein were found in blade gametophytes in all samples. Blade gametophytes harvested last year and stored at -20 ℃ showed only minor differences in expression of proteins when compared with those harvested in different seasons.
文摘The effect of cold acclimation on the freezing resistance and total soluble protein content in Populus tomentosa seedlings was studied for the first time in this paper. The results showed that in order to acquire higher freezing resistance, cold acclimation of Populus tomentosa seedlings might get through three periods, in which temperatures were -1℃、-2℃and -3℃ respectively. The freezing resistance of seedlings began to be induced by -1℃,but the third period of cold acclimation at -3℃ had most effect on the development of seedling freezing resistance. Suitable light could increase the freezing resistance and the content of total soluble protein of seedlings during cold acclimation, whereas 20 mg·L -1 of cycloheximide pretreatment of seedlings obviously decreased the freezing resistance and the content of total soluble protein. Further analysis found that the enhancement of total soluble protein content was closely related to the freezing resistance during cold acclimation.
