A partial common carp genomic library was constructed.45 positive clones were isolated from screening about 2000 clones of the genomic library with a (CA) 15 probe labelled at the 5’ end with γ 32 P ATP.Sequencing o...A partial common carp genomic library was constructed.45 positive clones were isolated from screening about 2000 clones of the genomic library with a (CA) 15 probe labelled at the 5’ end with γ 32 P ATP.Sequencing of these clones was performed with automated sequencer,and 22 microsatellites were isolated.17 primers were designed based on unique sequences flanking each motif with the software Primer3.PCR on Cyprinus carpio haematopterus was carried out with these primers,and all gave expected bands.Annealing temperature of these primers was between 50℃ and 53℃.展开更多
选择12个斑马鱼功能基因的微卫星标记和12个鲤鱼微卫星标记,检测黑龙江鲤(Cyprinus carpiohaematopterusTemminck et Schlegel)、散鳞镜鲤(C.carpio)、荷包红鲤抗寒品系(C.carpiovar.wuyuanensis)、松浦鲤(C.carpioSongpu carp)的群体...选择12个斑马鱼功能基因的微卫星标记和12个鲤鱼微卫星标记,检测黑龙江鲤(Cyprinus carpiohaematopterusTemminck et Schlegel)、散鳞镜鲤(C.carpio)、荷包红鲤抗寒品系(C.carpiovar.wuyuanensis)、松浦鲤(C.carpioSongpu carp)的群体遗传多样性。共检测到3882个扩增片段,长度为126～489bp,在群体间扩增出1～5个等位基因不等,共计59个等位基因,平均等位基因2.46个。数据经PHYLIP V3.6软件估算和MEGA3软件作图,确立4个群体间的亲缘关系。并应用Bootstrap检验估计系统树中节点的自引导值,并进行了系统发生分析。结果表明①4个群体检测的有效等位基因数都在55个以上,平均观测杂合度为0.36～0.43,平均期望杂合度为0.49～0.53,平均多态信息含量为0.21～0.25,说明这几个群体多态性属于中度偏高水平,遗传多样性较高;②群体间相似系数都在0.84以上,遗传距离较近,为0.067～0.170,与前人研究结果一致。聚类分析显示,松浦鲤与散鳞镜鲤亲缘关系最近,荷包红鲤抗寒品系与它们的亲缘关系较黑龙江鲤更近;③在3个斑马鱼功能基因相关的微卫星位点上,黑龙江鲤缺失特异扩增条带,这可能与几种鱼类的育成史有关。展开更多
文摘A partial common carp genomic library was constructed.45 positive clones were isolated from screening about 2000 clones of the genomic library with a (CA) 15 probe labelled at the 5’ end with γ 32 P ATP.Sequencing of these clones was performed with automated sequencer,and 22 microsatellites were isolated.17 primers were designed based on unique sequences flanking each motif with the software Primer3.PCR on Cyprinus carpio haematopterus was carried out with these primers,and all gave expected bands.Annealing temperature of these primers was between 50℃ and 53℃.
