Thymosin α 1 was synthesized manually. The washing condition and the test of the degree of coupling were studied. In addition, the analytical method of crude product was set up. Thymosin α 1 was synthesized with the...Thymosin α 1 was synthesized manually. The washing condition and the test of the degree of coupling were studied. In addition, the analytical method of crude product was set up. Thymosin α 1 was synthesized with the solid-phase method, by using the base-liable Fmoc group to protect the α-amino acid and N,N′-diisopropylcarbodiimide (DIC) as the coupling regent. The crude product was analyzed by reversed-phase high performance liquid chromatography, SDS-PAGE and liquid chromatography/mass spectroscopy.The synthesis yield was 84.89%. The analytical results indicated that the crude product contained much thymosin α 1, and the purity was 50.3%. DIC solid-phase chemical synthesis of thymosin α 1 was applicable, and the yield was improved greatly compared to other known methods.展开更多
The title compound was obtained as a by product in a contraction reaction by using DIC as a coupling reagent. X ray diffraction shows the title compound is a pyrrolinenitroxide radical urea. Two crystallographically i...The title compound was obtained as a by product in a contraction reaction by using DIC as a coupling reagent. X ray diffraction shows the title compound is a pyrrolinenitroxide radical urea. Two crystallographically independent molecules possess different conformations but the identical bond distances and angles. Based on the molecular structure a possible reaction mechanism is proposed for the reaction of DIC and carboxylic acid.展开更多
文摘Thymosin α 1 was synthesized manually. The washing condition and the test of the degree of coupling were studied. In addition, the analytical method of crude product was set up. Thymosin α 1 was synthesized with the solid-phase method, by using the base-liable Fmoc group to protect the α-amino acid and N,N′-diisopropylcarbodiimide (DIC) as the coupling regent. The crude product was analyzed by reversed-phase high performance liquid chromatography, SDS-PAGE and liquid chromatography/mass spectroscopy.The synthesis yield was 84.89%. The analytical results indicated that the crude product contained much thymosin α 1, and the purity was 50.3%. DIC solid-phase chemical synthesis of thymosin α 1 was applicable, and the yield was improved greatly compared to other known methods.
文摘The title compound was obtained as a by product in a contraction reaction by using DIC as a coupling reagent. X ray diffraction shows the title compound is a pyrrolinenitroxide radical urea. Two crystallographically independent molecules possess different conformations but the identical bond distances and angles. Based on the molecular structure a possible reaction mechanism is proposed for the reaction of DIC and carboxylic acid.
