近日，浸入式戏剧（Immersive theatre）《不眠之夜》（Sleep no more）在上海未演先热，12小时斩获百万票房，一万张预售票迅即售罄。这部风靡全球的作品到底有何魅力？浸入式戏剧到底是一种怎样的戏剧形式？本刊采访到了Punchdrunk艺...近日，浸入式戏剧（Immersive theatre）《不眠之夜》（Sleep no more）在上海未演先热，12小时斩获百万票房，一万张预售票迅即售罄。这部风靡全球的作品到底有何魅力？浸入式戏剧到底是一种怎样的戏剧形式？本刊采访到了Punchdrunk艺术总监菲利克斯·巴雷特（Felix Barrett），让他来为我们解答这些疑问。展开更多
AIM: To study the difference of gene expression profile changes in Barrett's esophagus (BE) and cardia intestinal metaplasia (CIM) and to screen the novel genes in the early stage by cDNA microarray.METHODS: cDNA ...AIM: To study the difference of gene expression profile changes in Barrett's esophagus (BE) and cardia intestinal metaplasia (CIM) and to screen the novel genes in the early stage by cDNA microarray.METHODS: cDNA retrotranscribed from an equal amount of mRNA from BE and CIM epithelial tissues was labeled with Cy3 and Cy5 fluorescence as probes. The mixed probe was hybridized with three pieces of BiostarH-40 s double dot human whole gene chip. The chips were scanned with a ScanArray 4000. The acquired images were analyzed using GenePix Pro 3.0 software.RESULTS: A total of 141 genes were screened out that exhibited different expression in all three chips. There were 74 upregulated and 67 downregulated genes in gene expression profiles of BE which were two times of that in CIM.CONCLUSION: There is a difference in gene expression level between BE and CIM epithelia. These 141 genes probably relate to the occurrence and development of BE and the progression to adenocarcinoma.展开更多
文摘近日，浸入式戏剧（Immersive theatre）《不眠之夜》（Sleep no more）在上海未演先热，12小时斩获百万票房，一万张预售票迅即售罄。这部风靡全球的作品到底有何魅力？浸入式戏剧到底是一种怎样的戏剧形式？本刊采访到了Punchdrunk艺术总监菲利克斯·巴雷特（Felix Barrett），让他来为我们解答这些疑问。
基金Supported by the Clinical Principal Discipline Foundation of Public Health Ministry of China,No.20012130
文摘AIM: To study the difference of gene expression profile changes in Barrett's esophagus (BE) and cardia intestinal metaplasia (CIM) and to screen the novel genes in the early stage by cDNA microarray.METHODS: cDNA retrotranscribed from an equal amount of mRNA from BE and CIM epithelial tissues was labeled with Cy3 and Cy5 fluorescence as probes. The mixed probe was hybridized with three pieces of BiostarH-40 s double dot human whole gene chip. The chips were scanned with a ScanArray 4000. The acquired images were analyzed using GenePix Pro 3.0 software.RESULTS: A total of 141 genes were screened out that exhibited different expression in all three chips. There were 74 upregulated and 67 downregulated genes in gene expression profiles of BE which were two times of that in CIM.CONCLUSION: There is a difference in gene expression level between BE and CIM epithelia. These 141 genes probably relate to the occurrence and development of BE and the progression to adenocarcinoma.
